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Calcium-dependent Membrane Penetration Is a Hallmark of theC2 Domain…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9668093.pdf20 Apr 2002: Avanti) used at 20 mM, have no effect on cPLA2C2 binding toPC vesicles. ... B, O-phospho-L-serine (Sigma) at 20 mM has no influenceon PC:PE:PS vesicle binding by SytIC2A. -
Catalytic Domain of Phosphoinositide-specificPhospholipase C…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9565585.pdf20 Apr 2002: Received for publication, November 20, 1997, and in revised form, February 18, 1998). ... The data are averages of twomeasurements that did not differ more than 20%. -
Crystal Structure of a Calcium-Phospholipid Binding Domainfrom…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9430701.pdf20 Apr 2002: For the heavy atom derivatives, crystalswere soaked for 20 h in the reservoir solution containing 1 mM K2PtCl4(data set K2PtCl4 (I)) for 5 h in 5 mM K2PtCl4 (data set ... 1995) Trends Biochem. Sci. 20, 272–27667. Murray, D., Ben-Tal, N., Honig, B., and -
Proc. Natl. Acad. Sci. USAVol. 94, pp. 9637–9642, September ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9275175.pdf20 Apr 2002: Communicated by Max F. Perutz, Medical Research Council, Cambridge, United Kingdom, June 20, 1997 (received for review April 2, 1997). ... 20. Leslie, A. G. W. (1992) Joint CCP4 and ESF-EACMB Newsletteron Protein Crystallography (Daresbury Lab., -
A Ternary Metal Binding Site in the C2 Domain ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9062102.pdf20 Apr 2002: 1 mM lanthanum (III)chloride, or 20 mM barium acetate for 6, 2, and 11 h,respectively. -
Structural Mapping of the Catalytic Mechanism for a…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9048554.pdf20 Apr 2002: 4.6)〈I/σ(I)〉b 24.1(5.1) 15.7(4.3) 20.6(4.4) 17.2(4.9). ... 2.95Rfactor(%)a 21.7 20.0 21.5 21.2Rfree(%)b 27.2 25.7 27.0 27.4no. -
cdf519 5057..5068
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12356722.pdf12 Oct 2002: POPE/POPA (50:20:30) vesicles was 1.2 6 0.3 nM. ... pockets on POPC/POPE/POPS (50:20:30)vesicles in the absence of phosphoinositide. -
PII: S1097-2765(01)00372-0
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11684018.pdf20 Apr 2002: Isothermal Titration CalorimetryAcknowledgmentsDi-C4-PtdIns(3)P (1 mM) in running buffer (20 mM Tris [pH 7.4] and. ... Johnson for help with calorimetry, and D.20 C, with an injection volume of 6 l. -
Tyrosine Residues in Phospholipase C�2 Essential for the…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11606584.pdf20 Apr 2002: Important Tyrosine Residues in PLC247984. (Lck) imidazole in 20 mM Hepes, pH 8.0. ... After induction with 0.2 mM isopropyl-1-thio--D-galacto-pyranoside (Calbiochem), cells were grown for 18 h at 20 C. -
x96255u149
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11090628.pdf20 Apr 2002: CCP4,20), and four times with 50 ml of solution 3 (20 mM Tris [pH 7.5], 1994). ... of phosphatidylinositol- 3-kinase. Cancer Res. 54, 2419–2423.Med. Res. Rev. 20, 28–57.
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