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1 - 10 of 123 search results for TALK:PC53 20 where 0 match all words and 123 match some words.

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  2. Model Building and Refinement Practical Introduction In this…

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ed-lowe-mb.docx
    27 Apr 2016: Under “Weight Matrix”, set the Refinement Weight to 20. 2.2. Automatic model building will only rarely produce a model which is both complete and correct.
    By: Edward Lowe

  3. Model Building and Refinement Practical Introduction In this…

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/mbar.docx
    27 Apr 2016: Under “Weight Matrix”, set the Refinement Weight to 20. 2.2. Automatic model building will only rarely produce a model which is both complete and correct.
    By: Edward Lowe

  4. Madeline Lancaster Cerebral organoids Version 2.0 Materials ·…

    https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/resources/Cerebral-organoids-2-0.docx
    25 May 2015: Day 5. hES media. Day 20 Day 40. dissolved Matrigel. Day 13.
    By: Lancaster,Madeline A.

  5. Madeline A. Lancaster Embedding and sectioning tissues in gelatin ...

    https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Gelatin-embedding.docx
    29 Oct 2012: Fix at 4C for 15-20 min. 2. Wash 3X for 10 min in PBS.
    By: Lancaster,Madeline A.

  6. Madeline A. Lancaster Culturing feeder-dependent human iPS/ES cells…

    https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Culturing-human-ES-and-iPS-cells.docx
    21 Mar 2014: 20 ng/ml bFGF (Add just before use). MEF Media (500 ml) – Store at 4C up to 2 weeks. ... 20 mg Dispase. 40 ml DMEM/F-12. Filtered using 0.22 um filter.

  7. Madeline Lancaster Generation of cerebral organoids using the SCT ...

    https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Cerebral-organoids-kit.docx
    17 Apr 2023: c. Place parafilm sheet in a dish in the 37 incubator for 20 min to allow matrigel to polymerize.
    By: Lancaster,Madeline A.

  8. Madeline Lancaster Cerebral organoids Version 2.1 from feeder-free…

    https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Cerebral-organoids-2-1-FF.docx
    17 Apr 2023: Matrigel droplet. Spinning bioreactor/shaker. Day 5. hES media. Day 20 Day 40. ... Matrigel droplet. Spinning bioreactor/shaker. Day 5. hES media. Day 20. Day 40.
    By: Lancaster,Madeline A.

  9. Madeline Lancaster Fixation, cryosectioning and immunostaining brain…

    https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Analyzing-brain-organoids.docx
    1 Aug 2019: Fix at 4C for 15-20 min. 2. Wash 3X for 10 min in PBS.
    By: Lancaster,Madeline A.

  10. 31/10/2018 Carter lab TIRF microscopy Single Molecule Assay 5x ...

    https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_TIRF_protocol.docx
    28 Jan 2020: 15 mg Glucose Oxidase 2mg Catalase (both in -20) into 250 μL DLB. ... Don’t pipette up and down. Could use a cut 20 μL tip for this.
    By: MRC

  11. Carter lab insect cell ZZ-tag prep (2020) Buffers Wash ...

    https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_Sf9_dynein_prep.docx
    24 Jan 2020: Gel-filtration on AKTAmicro. -Column is stored in water 0.05% NaN3. If stored in 20% ethanol, first wash into water before washing into buffer. ... Remove column and replace caps on either end. -Put inlet A1 into 20% ethanol, pumpwash.
    By: Max Schlager

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