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16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at

16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at

17 Mar 2003: 3 246,217 67,191 99.5 (100) 24.0 (4.4) 6.4 (27.1)SeMet l3 (0.9712) 2.3 248,253 67,292 99.7 (100) 22.9 ... Neuron 24, 143–154. Schmid, S.L. (1997). Clathrin-coated vesicle formation and proteinsorting: an integrated process.

17 Mar 2003: Cell, Vol. 104, 71–81, January 12, 2001, Copyright 2001 by Cell Press. Complexins Regulate a Late Step in Ca21-DependentNeurotransmitter Release. cellular Ca21 concentration. Usually, only a fraction oftethered vesicles in any given synapse is

21 Mar 2003: J Biol Chem 1996, 271:27979-27982. 24. Liu JP, Yajima Y, Li H, Ackland S, Akita Y, Stewart J, et al.: Molecularinteractions between dynamin and G-protein beta gamma-subunits ... Cells were plated 24 h prior to trans-fection (day 0) at a density of 1.4

21 Mar 2003: Correspondence: Harvey T. McMahonE-mail: hmm@mrc-lmb.cam.ac.uk. Received: 3 March 1998Revised: 24 April 1998Accepted: 5 May 1998. ... synapse. Cell 1994, 79:717-727. 24. DeBello WM, Betz H, Augustine GJ: Synaptotagmin andneurotransmitter release.

4 Apr 2003: x (A) 2 (4 (0 b&z) cp (dd’. Median 1.3 0.9 0.28 0.29 S.D.d 2.1 1.1 0.24 0.31. ... Range O&24.3 0.0-8.3 O&1.11 O.Gl.99. “All the figures shown were calculated after each whole repeat had been aligned to the reference structure.

9 May 2003: 24. individual yellow and cyan emission intensities. The ratio faithfully reflects reciprocal.

13 Jun 2003: To whom correspondence should be addressed. E-mail: jyl@mrc-lmb.cam.ac.uk. www.pnas.orgcgidoi10.1073pnas.1330742100 PNAS June 24, 2003 vol. ... Lowe, J. (1999) Struct.Folding Des. 7, 1181–1187. 24. Van Duyne, G.

19 Jun 2003: 4.3 Å 47.9 63.8 64.3 68.4 33.24.3–4.0 Å 56.5 71.8 71.3 77.2 41.6. ... 205, 137–142 (1986). 24. Imoto, K. et al. Location of a d-subunit region determining ion transport through the acetylcholine.