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11 May 2007: If using antibodies to label the protein(s) ofinterest, either monoclonal or mono-specificpolyclonal antibodies (raised against shortpeptide sequences of 8 to 20 amino acids)are preferable to broad specificity ... While the overall trends and

15 Feb 2007: selecting flow from a multichambered valve-controlledmechanical fed reservoir. Excitation light was provided bya 20-mW Argon Krypton laser. ... Each analog plot is representative of atleast three experiments, with each trace showing the average for

15 Aug 2007: For fractionation, cytosolic proteins were selectively extracted on icefor 5 min with 1 ml/35-mm dish of 100 g/ml digitonin in KHM buffer (110 mMKAc, 20 mM HEPES, pH ... Cross-linker was quenched with 20 mM Tris-Cl, pH 7.5, the cells were lysed in1% SDS

25 Jun 2007: Crystals were equilibrated in 20% glycerol for cooling to 100K. The crystal asymmetric unit contains one molecule, and the crystals belong to spacegroup P3121, cell dimensions a = b = 49.6Å, c = ... Sedimentation was at 11,000 rev/min, 20.0C, with

25 Jun 2007: EpsinR L10E overexpression in COS cells. Myc-EpsinR L10E with endogenous AP1 and internalised transferrin in COS cells. Insertion of a glutamic acid (E) in place of the hydrophobic leucine (L) is predicted to affect epsinR's ability to bend membranes

25 Jul 2007: 18:30 Dinner 20:00 Opening event: Concert at Schloß Waldthausen by „Trio con brio“ (piano trio) 22:00 Get together in the „Weinkeller“ of Schloß Waldthausen Thursday, Oct 11th, 2007

21 Aug 2007: to contain up to 20 different component in appropriate stoichiometries that ensure efficient targeting, fusion and recycling/refilling of endocytosed vesicles. ... over 20 different proteins bind to the AP2 a-appendage). When we plot proteins known to be

25 Jun 2007: Current Biol. Oct 26;14(20):1791-800. (see also Research Roundup? Snx1 hugs the curves?

15 May 2007: 14,19,20). The IVF motif also contributes to the intra-. molecular interaction but with significant difference among.

28 Sep 2007: 3,000. 0. 20. 40. 60. 80. 100. 180160140120100806040200 200Time (min). PtdIns(4)P. ... The. column was extensively washed with 20 mM HEPES (pH 7.5), 700 mM NaCl,.