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doi:10.1016/j.str.2007.05.002
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf2 Jun 2007: mM NaCl, 20 mM HEPES [pH 7.5], 2.5 mM DTT), and the GST tag. ... for 30 min at room temperature in Beckman 7 3 20 mm polycarbonate. -
Negative-staining protocol for EM: Harvey McMahon lab
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Negative%20stain%20protocol.html25 Jun 2007: Usually I mix at least 20 ul; otherwise evaporation can drastically change the salt content. ... Pipette drops of UrAc (20-40 ul each) onto ParafilmÑtwo drops per sample. -
Structural insight into the ESCRT-I/-II link and itsrole in ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17215868.pdf14 Jun 2007: of clustered ubiquitinated cargo before cargo sorting intoReceived: 22 August 2006; accepted: 20 November 2006; publishedonline: 11 January 2007. ... 0.19. 0.20. 0.21. 0.23. 0.24. 0.25. 0.26. Ani. sotr. opy. Concentration (nM). -
PowerPoint Presentation
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/Interactomes/NetworkWebTalk_files/NetworkWebTalk.html.ppt21 Aug 2007: to contain up to 20 different component in appropriate stoichiometries that ensure efficient targeting, fusion and recycling/refilling of endocytosed vesicles. ... over 20 different proteins bind to the AP2 a-appendage). When we plot proteins known to be -
Tumour prevention by a single antibody domaintargeting the…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17568777.pdf22 Jul 2007: Thepurification procedure of the complex was the same as for the scFvdescribed in Supplementary data, except the extraction buffer(25 mM Na phosphate, pH 7.4, 300 mM NaCl, 20 mM ... imidazole)and gel filtration buffer (20 mM Tris–HCl pH 8.0 at -
Monolayer Assay Protocol
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/mono.html25 Jun 2007: Purified clathrin. Clathrin should be dialyzed into HKM buffer and centrifuged for 20 minutes at 100000 gmax (e.g., 45000 r.p.m. -
"Rational Design and Evaluation of FRET Experiments to Measure…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/40_Snapp_EL_Curr_Prot_2006.pdf11 May 2007: If using antibodies to label the protein(s) ofinterest, either monoclonal or mono-specificpolyclonal antibodies (raised against shortpeptide sequences of 8 to 20 amino acids)are preferable to broad specificity ... While the overall trends and -
The structural basis of novel endosome anchoringactivity of KIF16B ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17641687.pdf23 Jul 2007: 40. 80. 120. 0 20 40 60. Req. (R. U). PX concentration (nM). ... layer to the level of its penetration into POPC/POPE (80:20). monolayer. -
Structural and Membrane Binding Analysis of the PhoxHomology Domain…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17581820.pdf8 Dec 2007: However, K338Aand R349A showed 3- and 4-fold reduced affinity, respectively,for more anionic POPC/POPE/POPS/PtdIns(4)P (57:20:20:3)vesicles. ... 3)P (77:20:3) (f), POPC/POPE/PtdIns(5)P (77:20:3) (Œ),and POPC/POPE/POPS (60:20:20) (") monolayers. -
doi:10.1016/j.jmb.2007.08.056
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Oliva_FtsZ_JMB_2007.pdf11 Oct 2007: The nucleotide content was measured asdescribed.20. Untagged, full-length ftsZ from B. ... J. Biol. Chem.278, 37664–37671. 20. Oliva, M. A., Huecas, S., Palacios, J.
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