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15 Feb 2007: selecting flow from a multichambered valve-controlledmechanical fed reservoir. Excitation light was provided bya 20-mW Argon Krypton laser. ... Each analog plot is representative of atleast three experiments, with each trace showing the average for

2 Apr 2007: Purified tubulins were desalted into PEM containing 20 μM GDP before storage in liquid nitrogen. ... Enzyme reactions were done in 20 mM PIPES pH 6.9, 2 mM MgCl2, using quartz fluorimeter cuvettes.

6 Apr 2007: 0 5 10 15 20. 0 5 10 15 20. 0 5 10 15 20-10. ... Themicrotubule plus end is toward the top of thepage. (Left) Cryo-EM map (20).

11 May 2007: If using antibodies to label the protein(s) ofinterest, either monoclonal or mono-specificpolyclonal antibodies (raised against shortpeptide sequences of 8 to 20 amino acids)are preferable to broad specificity ... While the overall trends and

15 May 2007: 14,19,20). The IVF motif also contributes to the intra-. molecular interaction but with significant difference among.

2 Jun 2007: Samples at different. loading concentrations were sedimented at 10,000 rpm at 20.0 ºC in double sector cells.

2 Jun 2007: mM NaCl, 20 mM HEPES [pH 7.5], 2.5 mM DTT), and the GST tag. ... for 30 min at room temperature in Beckman 7 3 20 mm polycarbonate.

14 Jun 2007: Chem. 279, 36059–36071. 20. Bache, K.G., Slagsvold, T., Cabezas, A., Rosendal, K.R., Raiborg, C. ... and Sundquist, W.I. (2004) Annu. Rev. Cell Dev. Biol. 20, 395–42553.

14 Jun 2007: of clustered ubiquitinated cargo before cargo sorting intoReceived: 22 August 2006; accepted: 20 November 2006; publishedonline: 11 January 2007. ... 0.19. 0.20. 0.21. 0.23. 0.24. 0.25. 0.26. Ani. sotr. opy. Concentration (nM).

14 Jun 2007: Recruitment of ESCRT-0 to the endosomal membrane. The N-terminal FYVE domain of HRS mediates recog-nition of PtdIns3P, a highly abundant phosphoinositide in endosomal membranes18–20. ... Clathrin acts as an organizer of the ESCRT pathway. HRS is highly