Search

25 Jun 2007: Mix by inversion to ensure homogeneity. 11. Spin in a 70Ti rotor at 25000rpm for 20 minutes. ... Run at 1ml/min (Abs. 0.5) and collect 2ml fractions. Clathrin elutes at around fraction 20.

25 Jun 2007: Crystals were equilibrated in 20% glycerol for cooling to 100K. The crystal asymmetric unit contains one molecule, and the crystals belong to spacegroup P3121, cell dimensions a = b = 49.6Å, c = ... Sedimentation was at 11,000 rev/min, 20.0C, with

25 Jun 2007: NB: the filtration process usually leads to loss of around 100-200 ul of liquid, and 10-20% of the lipid in the reamining volume.

25 Jun 2007: EpsinR L10E overexpression in COS cells. Myc-EpsinR L10E with endogenous AP1 and internalised transferrin in COS cells. Insertion of a glutamic acid (E) in place of the hydrophobic leucine (L) is predicted to affect epsinR's ability to bend membranes

25 Jun 2007: Usually I mix at least 20 ul; otherwise evaporation can drastically change the salt content. ... Pipette drops of UrAc (20-40 ul each) onto ParafilmÑtwo drops per sample.

25 Jun 2007: Purified clathrin. Clathrin should be dialyzed into HKM buffer and centrifuged for 20 minutes at 100000 gmax (e.g., 45000 r.p.m.

2 Apr 2007: Purified tubulins were desalted into PEM containing 20 μM GDP before storage in liquid nitrogen. ... Enzyme reactions were done in 20 mM PIPES pH 6.9, 2 mM MgCl2, using quartz fluorimeter cuvettes.

6 Apr 2007: 0 5 10 15 20. 0 5 10 15 20. 0 5 10 15 20-10. ... Themicrotubule plus end is toward the top of thepage. (Left) Cryo-EM map (20).

25 Jul 2007: 18:30 Dinner 20:00 Opening event: Concert at Schloß Waldthausen by „Trio con brio“ (piano trio) 22:00 Get together in the „Weinkeller“ of Schloß Waldthausen Thursday, Oct 11th, 2007

2 Jun 2007: Samples at different. loading concentrations were sedimented at 10,000 rpm at 20.0 ºC in double sector cells.

25 Jun 2007: Current Biol. Oct 26;14(20):1791-800. (see also Research Roundup? Snx1 hugs the curves?

14 Jun 2007: Chem. 279, 36059–36071. 20. Bache, K.G., Slagsvold, T., Cabezas, A., Rosendal, K.R., Raiborg, C. ... and Sundquist, W.I. (2004) Annu. Rev. Cell Dev. Biol. 20, 395–42553.

8 Dec 2007: Concentration (µM)0 20 40 60 80 100. I–I 0. (arb. itrar. ... interaction. Indeed, CHMP1Bbinds human Vps4A MIT domain with a Kd of 20 mM (ref.

8 Dec 2007: 0. 20. 40. 60. 80. 100. 120. 140. 0 50 100 150p85ni (nM). ... 65, 4562 (2005).20. S. J. Isakoff et al., Cancer Res. 65, 10992 (2005).21.

15 Oct 2007: and then incubated with pig brain dynactin complex at 30 1Cfor a further 20 min. ... 20 mg/ml) and 15% v/v heat-inactivated fetal calf serum (Invitrogen,Paisley, UK).

23 Jul 2007: 40. 80. 120. 0 20 40 60. Req. (R. U). PX concentration (nM). ... layer to the level of its penetration into POPC/POPE (80:20). monolayer.

4 Oct 2007: 2007 European Molecular Biology Organization The EMBO Journal VOL 26 | NO 20 | 2007. ... 2007 European Molecular Biology Organization The EMBO Journal VOL 26 | NO 20 | 2007 4415.

8 Dec 2007: However, K338Aand R349A showed 3- and 4-fold reduced affinity, respectively,for more anionic POPC/POPE/POPS/PtdIns(4)P (57:20:20:3)vesicles. ... 3)P (77:20:3) (f), POPC/POPE/PtdIns(5)P (77:20:3) (Œ),and POPC/POPE/POPS (60:20:20) (") monolayers.

21 Aug 2007: to contain up to 20 different component in appropriate stoichiometries that ensure efficient targeting, fusion and recycling/refilling of endocytosed vesicles. ... over 20 different proteins bind to the AP2 a-appendage). When we plot proteins known to be

11 Oct 2007: The nucleotide content was measured asdescribed.20. Untagged, full-length ftsZ from B. ... J. Biol. Chem.278, 37664–37671. 20. Oliva, M. A., Huecas, S., Palacios, J.

22 Aug 2007: These data come from the following papers: (AP2, clathrin)1,20, (dynamin, clathrin)21,22,. ... endocytosis in Drosophila. Genes Dev. 15, 2967-2979 (2001). 20. Rappoport, J.

2 Jun 2007: mM NaCl, 20 mM HEPES [pH 7.5], 2.5 mM DTT), and the GST tag. ... for 30 min at room temperature in Beckman 7 3 20 mm polycarbonate.

22 Aug 2007: Such attachments can be used in manydiverse pathways (for example, actin polymerization associated withvesicle scission and motility18–20). ... Nature Cell Biol. 4,691–698 (2002). 20. Kaksonen, M., Toret, C. P. &

28 Sep 2007: 3,000. 0. 20. 40. 60. 80. 100. 180160140120100806040200 200Time (min). PtdIns(4)P. ... The. column was extensively washed with 20 mM HEPES (pH 7.5), 700 mM NaCl,.

31 Oct 2007: Apart from the movement of the necklinker, the most striking difference between the ADP and ATP-like states, a 20 rotation of the core domain relative to 4, isalso evident in ... EMBO J. 20, 2611-2618. Yun, M., Bronner, C. E., Park, C.

14 Jun 2007: of clustered ubiquitinated cargo before cargo sorting intoReceived: 22 August 2006; accepted: 20 November 2006; publishedonline: 11 January 2007. ... 0.19. 0.20. 0.21. 0.23. 0.24. 0.25. 0.26. Ani. sotr. opy. Concentration (nM).

15 Feb 2007: selecting flow from a multichambered valve-controlledmechanical fed reservoir. Excitation light was provided bya 20-mW Argon Krypton laser. ... Each analog plot is representative of atleast three experiments, with each trace showing the average for

24 Sep 2007: º) 90, 106.5, 90. Wavelength 0.97962. Resolution (Å) 20 – 3.1 (3.2 – 3.1). ... www.nature.com/nature 20. Supplementary notes / Supplementary References. 23. Lenzen, C., Cool, R.

22 Jul 2007: Thepurification procedure of the complex was the same as for the scFvdescribed in Supplementary data, except the extraction buffer(25 mM Na phosphate, pH 7.4, 300 mM NaCl, 20 mM ... imidazole)and gel filtration buffer (20 mM Tris–HCl pH 8.0 at

11 May 2007: If using antibodies to label the protein(s) ofinterest, either monoclonal or mono-specificpolyclonal antibodies (raised against shortpeptide sequences of 8 to 20 amino acids)are preferable to broad specificity ... While the overall trends and

14 Jun 2007: Recruitment of ESCRT-0 to the endosomal membrane. The N-terminal FYVE domain of HRS mediates recog-nition of PtdIns3P, a highly abundant phosphoinositide in endosomal membranes18–20. ... Clathrin acts as an organizer of the ESCRT pathway. HRS is highly

15 Aug 2007: For fractionation, cytosolic proteins were selectively extracted on icefor 5 min with 1 ml/35-mm dish of 100 g/ml digitonin in KHM buffer (110 mMKAc, 20 mM HEPES, pH ... Cross-linker was quenched with 20 mM Tris-Cl, pH 7.5, the cells were lysed in1% SDS

15 May 2007: 14,19,20). The IVF motif also contributes to the intra-. molecular interaction but with significant difference among.