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554 Research Paper Inhibition of receptor-mediated endocytosis by the …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf?cmd=Retrieve&db=PubMed&list_uids=9348539&dopt=Abstract21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se, -
Nicotinic Acetylcholine Receptor at 4.6 Å Resolution: Transverse…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jmb99.pdf15 Sep 2003: Thus with asingle image, there was too much noise for few, ifany, of the layer-lines to be distinguished convin-cingly beyond a resolution of about 20 AÊ. ... Common to all views are the 20 AÊwide vestibules on either side of the membrane,and a more -
Hidden Markov Model search with amphiphysin
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/BARdomains/hmm_runs.htm11 Dec 2003: gi|17861564|gb|AAL39259.1|_5:228. 224. 5.90E-22. :GH12888p [Drosophila melanogaster]. gi|2921412|gb|AAC04766.1|_1:175. 175. 2.30E-20. :EEN-B2-L2 [Homo sapiens]. ... gi|26343319|dbj|BAC35316.1|_20:165. 146. 1.40E-14. :unnamed protein product [Mus musculus] -
PII: S0304-3991(97)00070-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram97.pdf4 Apr 2003: The signal retention improved by only 10% at low resolution (20 A), but by progressively greater amounts at higher resolutions, up to - 40% at 9 8. ... 20]; variable twist has been corrected by ap- plying an innovative real-space algorithm in a study of -
doi:10.1016/S1097-2765(03)00246-6
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12887891.pdf5 Sep 2003: to-back interacting PB1 domains, with a freebuffer C (20 mM Tris, pH 7.4, 100 mM NaCl, and 5 mM DTT). ... C.D., Anderson, K.E., Butler, P.J., Lavenir, I., Perisic, O., Hawkins,GST/Par6b binding assays) and run on 20% Phast SDS gels. -
Membrane curvature sensing and induction: The function of BAR domains …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/BARdomains/BARsupp.pdf20 Nov 2003: Crystals were equilibrated in 20% glycerol for cooling to 100K. The crystal asymmetric unit contains one molecule, and the crystals belong to spacegroup P3121, cell dimensions a = b = 49.6Å, c = ... 1mM TCEP. Sedimentation was at 11,000 rev/min, 20.0C, -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/sedequil.pdf16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/26_Snapp_EL_JCB_2003.pdf16 Oct 2003: The. Jour. nal o. f Cel. l Bio. logy. JCB. Article. The Journal of Cell Biology, Volume 163, Number 2, October 27, 2003 257–269http://www.jcb.org/cgi/doi/10.1083/jcb.200306020 257. Formation of stacked ER cisternae by low affinity protein -
PII: 0896-6273(89)90235-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/neuron89.pdf11 Sep 2003: Usually there are four or more stretches of about 20 hydrophobic amino acids per subunit or domain: the correct length for an a-helix to span the hydrophobic lipid chains. ... the contacting helices are inclined to one another by even greater angles: -
Bb9e55.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Vallis_McMahon1999.pdf21 Mar 2003: Dynamin GTPase assay and sedimentation assayThese assays have been described previously [20,27] and furtherdetails are given in the Supplementary material. ... Multimerised dynamin was then sedimented by ultracentrifuga-tion at 80,000 g for 20 min.
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