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8 Dec 2003: 3.6. 2.9 x 10. 5. -20.0. -12.9. Ins(1,4,5)P. 3. 20 C. ... 20.4. -13.2. diC. 8. PtdIns(4,5)P. 2. 2.2. 4.6 x 10. 5.

16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023.

16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023.

20 Nov 2003: Crystals were equilibrated in 20% glycerol for cooling to 100K. The crystal asymmetric unit contains one molecule, and the crystals belong to spacegroup P3121, cell dimensions a = b = 49.6Å, c = ... 1mM TCEP. Sedimentation was at 11,000 rev/min, 20.0C,

11 Dec 2003: gi|17861564|gb|AAL39259.1|_5:228. 224. 5.90E-22. :GH12888p [Drosophila melanogaster]. gi|2921412|gb|AAC04766.1|_1:175. 175. 2.30E-20. :EEN-B2-L2 [Homo sapiens]. ... gi|26343319|dbj|BAC35316.1|_20:165. 146. 1.40E-14. :unnamed protein product [Mus musculus]

21 Mar 2003: Dynamin GTPase assay and sedimentation assayThese assays have been described previously [20,27] and furtherdetails are given in the Supplementary material. ... Multimerised dynamin was then sedimented by ultracentrifuga-tion at 80,000 g for 20 min.

13 Jun 2003: 20) and Trusca et al. (21), using a MBP-SulAfusion or a protein A-SulA fusion, respectively, found SulA doesinhibit the GTPase activity of FtsZ. ... The reservoir was 7.5%polyethylene glycol (PEG) 20,0007.5% PEG monomethyl ether55080 mM sodium formate0.1

21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se,

16 Jul 2003: and POPC/POPE/phos-phatidylinositol 3,4,5-trisphosphate (77:20:3) vesicles. ... a Increase in Kd relative to binding POPC/POPE/PtdIns(4,5)P2 (77:20:3) vesicles.b Not measurable.

27 Nov 2003: EMBO J 2001,20:5813-5821. 49. Hinshaw JE: Dynamin and its role in membrane fission.Annu Rev Cell Dev Biol 2000, 16:483-519. ... EMBO J2001, 20:1819-1828. Structural/functional homology between the bacterial and eukaryotic cytoskeletons Amos, van den Ent

21 Mar 2003: whereas glutamate release was essentially unaffected (96 9% ofmaximal; n = 3) in the presence of 20 µM cyclosporin A. ... Fol-lowing two washes, the synaptosomes were spun briefly a third timeand resuspended in lysis buffer (20 mM Hepes).

17 Mar 2003: vesicle endocytosis. J. Neurosci. 20, 8667–8676. Ye, W., and Lafer, E.M. ... I. Developmental expres-sion and cellular localization of the F1-20 protein and mRNA.

30 Jun 2003: EMBO J. 20, 5383 – 5391. 12 Ma, J. and Lindquist, S.

19 Jun 2003: holes, using a 300-kV field emission microscopeincorporating a top-entry liquid-helium-cooled stage10 (magnification, £36,800; defocusrange, 9,000–16,000 Å; dose, ,20 electrons Å22). ... 3H]diazofluorene. J. Biol. Chem. 273, 8659–8668 (1998). 20.

17 Mar 2003: presence of 20 ml 50% slurry of glutathione-following induction with 0.2 mM IPTG. ... Cell Biol. 20, 313–332.pathway in viral infection. EMBO J. 17, 4585–4593.

9 May 2003: least two chemosensory neurons (AWA and AWC) has been measured to -20 mV, which. ... membranes. After 15-20 minutes of equilibration the cells were transferred into fresh.

15 Sep 2003: Thus with asingle image, there was too much noise for few, ifany, of the layer-lines to be distinguished convin-cingly beyond a resolution of about 20 AÊ. ... Common to all views are the 20 AÊwide vestibules on either side of the membrane,and a more

9 Jan 2003: 20, 2003 213–222http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. ... All GST fu-sion proteins were purified from bacterial extracts by incubation with glu-tathione-Sepharose beads, followed by extensive washing with 20 mMHepes, pH 7.4, 150 mM NaCl,

17 Mar 2003: controls, as well as release phase while the corresponding value in controlCPX I/II double mutant (2/2, 2/2; B) synapses (20,000-fold magnifi-. ... initial synaptic release probability is reduced in these concentrations of 0.5–20 mM, while the Mg21

4 Apr 2003: The signal retention improved by only 10% at low resolution (20 A), but by progressively greater amounts at higher resolutions, up to - 40% at 9 8. ... 20]; variable twist has been corrected by ap- plying an innovative real-space algorithm in a study of

5 Sep 2003: to-back interacting PB1 domains, with a freebuffer C (20 mM Tris, pH 7.4, 100 mM NaCl, and 5 mM DTT). ... C.D., Anderson, K.E., Butler, P.J., Lavenir, I., Perisic, O., Hawkins,GST/Par6b binding assays) and run on 20% Phast SDS gels.

27 Oct 2003: being represented by 3 of the 20 ESTs for human golgin-(C) The oligomerization state of the His6-GRIP domain derived by 245 in the current GenBank database. ... The complex was concentrated ton X-100 in PBS, blocked with 20% (v/v) fetal calf serum/0.25%.

11 Sep 2003: Usually there are four or more stretches of about 20 hydrophobic amino acids per subunit or domain: the correct length for an a-helix to span the hydrophobic lipid chains. ... the contacting helices are inclined to one another by even greater angles:

6 Feb 2003: factor inthe same proteoliposomes was only. 15–20% of the control,raising the possibility that its transport may require stimula-tory factor(s) not needed by Prl. ... Only after the synthesis of an additional 20 resi-dues did the PrP–Prl intermediate

21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se,

16 Oct 2003: The. Jour. nal o. f Cel. l Bio. logy. JCB. Article. The Journal of Cell Biology, Volume 163, Number 2, October 27, 2003 257–269http://www.jcb.org/cgi/doi/10.1083/jcb.200306020 257. Formation of stacked ER cisternae by low affinity protein