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"Rational Design and Evaluation of FRET Experiments to Measure…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/40_Snapp_EL_Curr_Prot_2006.pdf11 May 2007: Consider that a homogeneouslydistributed fluorescent protein must be presentat 200 nM to be visualized over backgroundcellular fluorescence (Niswender et al., 1995).Thus, experimental questions involving cellu-lar expression of a fluorescently -
, 130081, published 7 August 20133 2013 Open Biol. ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Open%20Biol.-2013-TakedaCombined.pdf25 Sep 2013: input IP. GFPAni1-409GFP. SyndFLAG. Ani1-409GFP. GFP. 200. valu. e. 100. 200. ... Scale bars represent 1 mm and 200 nm for low magnification and high magnificationimages, respectively. -
1202.tif
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb84.pdf16 Jun 2006: 5) , andthe i r w i dth rema i ned fa i r l y constant (800 - 1 , 200 A). -
1256917combinedsupplement
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gligoris-SM.pdf14 Nov 2014: loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare) in 50 mM Tris pH 7.5, 150 mM NaCl, 1 mM βME. ... Crystals were cryo-protected by adding reservoir solution supplemented with 50-60 % (w/v) PEG 200, before flash freezing in liquid -
14821680963433 1..18
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/e21600-download.pdf19 Dec 2016: 200 mM NaCl, 1 mM DTT, 10% glycerol (v/v), 10 mM imidazole, pH 8.0) supplemented with DNase. ... Stock et al., 2005). After optimisation, the best crystals were obtained in 200 nl drops composed. -
15211199233993 1..45
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreBHussain2018.pdf15 Mar 2018: As the curvature of MreB filaments bound to liposomes is much greater (200 nm diameter. -
15591370691369 1..30
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf29 May 2019: A) Cells expressing GFP-PrP and GFP-PrP were incubated on. ice with 200 nM extracellular Alexa647-conjugated Nb and Alexa647 fluorescence was measured by flow cytometry. ... pro. port. ion o. f cells. time (min). 0 50 100 150 200. -
15919734904046 1..30
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020.pdf12 Jun 2020: 6Y4L). The heterodimer consists of EMC2 (residues 11–274), depicted in blue, and EMC9 (residues 1–200),. ... of atoms 9412. Protein residues 1906. Ramachandran Outliers (%) 0.49. Mean B-factors (2) 200.0. -
15955888524538 1..29
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020b.pdf24 Jul 2020: For correspondence:. rhegde@mrc-lmb.cam.ac.uk. †These authors contributed. equally to this work. Competing interest: See. page 25. Funding: See page 25. Received: 15 June 2020. Accepted: 10 July 2020. Published: 13 July 2020. Reviewing editor: -
16001658465475 1..36
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Collier_2020_elife-59560-v1.pdf15 Sep 2020: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 33. Received:
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