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https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf29 May 2019: A) Cells expressing GFP-PrP and GFP-PrP were incubated on. ice with 200 nM extracellular Alexa647-conjugated Nb and Alexa647 fluorescence was measured by flow cytometry. ... pro. port. ion o. f cells. time (min). 0 50 100 150 200. -
Activation of GCN2 by the ribosomal P-stalk
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/PNAS_2019.pdf7 Mar 2019: P1P2. GCN2. uL10. A. 200. kDa. 15012010085. 7060. 50. 4030. 25201510. -
Articleshttps://doi.org/10.1038/s41594-019-0196-z 1MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%202019%20NSMB.pdf4 Mar 2019: 150 200 250 300 350 400 450 500. 0. 1. Residue number (S. ... 1. 900 1,000 1,100 1,200. 200 250 300 350 400 450. -
Articleshttps://doi.org/10.1038/s41594-019-0196-z A folded…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%20NSMB%202019%20SI.pdf26 Feb 2019: reconstituted in buffer containing 40 mM NaCl, 2 mM MgCl2 (red) and doublet MukBEF (MukBEFD) reconstituted in buffer containing 200 mM NaCl (green). -
Biallelic Variants in ASNA1, Encoding a Cytosolic Targeting Factor of …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CircGen_2019.pdf1 Dec 2019: 1:400),5 mouse monoclonal anti-N-cadherin (Agilent Technologies #M3616, dilution 1:200), and. mouse monoclonal anti-desmin (Ventana Medical Systems #760-2513, prediluted). -
Coot Cryo-EM Practical
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/Coot-Cryo-EM.html8 Aug 2019: Calculate Modules Cryo-EM. Cryo-EM Multi-sharpen…. Use 10 levels to 200. OK. ... OK. Re-read the mtz file, but this time:. – In Amplitudes: choose FoutBlur_200.0. – -
Crystal Structures and Nuclear Magnetic Resonance Studies of the Apo…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/acs.biochem.9b00296.pdf31 Jul 2019: A common approach included single dropswith final volumes of 200 nL with a 1:1 ratio (100 nL of proteinand 100 nL of precipitant solution). -
Current Developments in Coot for Macromolecular Model Building…
https://www2.mrc-lmb.cam.ac.uk/coot/protein-science/Casanal_Lohkamp_Emsley_11112019-pe-2.pdf11 Nov 2019: e.g. 200 A2) before the fitting steps, as blurring allows a larger radius of. -
Cyrus Chothia (1942 – 2019) - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/cyrus-chothia-1942-2019/27 Nov 2019: His work on eukaryotes showed that the number of members in the 200 superfamilies, of the 1,200 that were known, correlates with the complexity of the organisms in which they -
Fast, simple, accessible and affordable: The future of cryo-EM - MRC…
https://www2.mrc-lmb.cam.ac.uk/fast-simple-accessible-and-affordable-the-future-of-cryo-em/11 Oct 2019: standard of 300 or 200 kiloelectron volts (keV) to 100 keV. ... keV. The group succeeded in doing this by reducing the voltage of a standard, commercial 200 kV Field Emission Gun (FEG) microscope to 100 kV.
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