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  2. Uncovering the action of a selective holophosphatase inhibitor - MRC…

    https://www2.mrc-lmb.cam.ac.uk/uncovering-action-selective-holophosphatase-inhibitor/
    Thumbnail for Uncovering the action of a selective holophosphatase inhibitor - MRC Laboratory of Molecular Biology 31 Jul 2017: There are over 200 different regulatory subunits that can combine with PP1 to form holophosphatases which dephosphorylate a wide range of proteins in cells. ... There are more than 200 such phosphatases in humans that could in principle be studied using
  3. LMB staff are cycle enthusiasts - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/lmb-staff-cycle-enthusiasts/
    Thumbnail for LMB staff are cycle enthusiasts - MRC Laboratory of Molecular Biology 20 Apr 2017: Published on. 20 April, 2017. The LMB won the National Cycling Challenge for the 200-499 employees category in 2016, with the team cycling 9,969 miles in the month of
  4. LMB cycles to first place in September Challenge - MRC Laboratory of…

    https://www2.mrc-lmb.cam.ac.uk/lmb-cycles-first-place-september-challenge/
    Thumbnail for LMB cycles to first place in September Challenge - MRC Laboratory of Molecular Biology 1 Nov 2017: This year’s Cycle September challenge was the main #YearoftheBike event organised by Love to Ride as part of the bicycle’s 200.
  5. Robotic crystallisation facility: overview

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/robot-overview.html
    5 Jun 2017: Drops are set with nanolitre dispensing robots (mainly TTP's MOSQUITO). A common approach is single drops of 200 nl final volume with 1:1 ratio (100 nl protein 100 nl ... Larger volume MAXI plates and the corresponding protocols are available for
  6. doi:10.1016/j.pbiomolbio.2004.07.009

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf
    22 Sep 2017: available: the IMPAX and ORYX systems from Douglas Instruments Ltd., UK, and theCyberlab 200, distributed by Gilson Inc., USA.
  7. Optimization of crystallization conditions with additive screening…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf
    9 Jan 2017: 200 nl protein 200 nl condition 100 nl additive). This late approach facilitates the screening at different concentrations of additives (by simply varying the volume of additive screen added) and implies
  8. Protocol for dragonfly

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/protocols_for_dragonfly.pdf
    13 Dec 2017: secMAXI 24 200 1.8 2 min 25 secMAXI 48 200 3 4 min 20 sec.
  9. Protocol for dragonfly copy

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_mosquito_additive.pdf
    13 Dec 2017: of tips Duration. 1 MRC plate 100 100 0 2 µL 2 16 104 2 min1 MRC plate 200 200 0 5 µL 3.8 31 104 2min 10 sec2 Costar ... plate 200 200 100 5 µL 3.8 31 200 3min 45 sec2 Costar plate 500 500 100 5 µL 7.4 60 200 4min 15 sec.
  10. Robotic crystallisation: Consumables

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/consumables.html
    5 Jun 2017: Swissci. MMX01-UVP. The MRC MAXI 1-drop plate for scale-up/optimization. Reservoir recommended volume: 200  µL.
  11. Structure of the hexagonal surface layer on Caulobacter crescentus…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf
    6 Apr 2017: Crystals wereflash-frozen in liquid nitrogen for data collection using an additional 25% (vol/vol)PEG 200 as cryo-protectant. ... 200 mesh grids in a Vitrobot Mark IV (FEICompany) maintained at 10 C at 100% relative humidity.

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