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9 Jan 2017: 200 nl protein 200 nl condition 100 nl additive). This late approach facilitates the screening at different concentrations of additives (by simply varying the volume of additive screen added) and implies

13 Jan 2017: 0 100 200 3001. 1.5. 2. 2.5. Time (s). YFP. /CFP. ... 2.5. YFP. /CFP. rat. io. 0 100 200 300 4001. 1.5.

19 Jan 2017: vector and a 3’ primer that anneals 200 bp downstream of the stop codon of the open reading frame. ... DTT). During the buffer change, 1:200 of SuperTEV (for His-tagged proteins) or 3C protease (for GST-tagged proteins) were added for overnight

6 Apr 2017: Crystals wereflash-frozen in liquid nitrogen for data collection using an additional 25% (vol/vol)PEG 200 as cryo-protectant. ... 200 mesh grids in a Vitrobot Mark IV (FEICompany) maintained at 10 C at 100% relative humidity.

20 Apr 2017: Published on. 20 April, 2017. The LMB won the National Cycling Challenge for the 200-499 employees category in 2016, with the team cycling 9,969 miles in the month of

3 May 2017: All images are at the same magnification (scale bar, 200 nm). ... However, these filaments are not the sameas the open-form filaments, with a much smaller interface buried surface area (BSA) of700 Å2, compared to 1,200 Å2 for 1FOf and PDB

5 Jun 2017: Drops are set with nanolitre dispensing robots (mainly TTP's MOSQUITO). A common approach is single drops of 200 nl final volume with 1:1 ratio (100 nl protein 100 nl ... Larger volume MAXI plates and the corresponding protocols are available for

5 Jun 2017: Swissci. MMX01-UVP. The MRC MAXI 1-drop plate for scale-up/optimization. Reservoir recommended volume: 200  µL.

31 Jul 2017: There are over 200 different regulatory subunits that can combine with PP1 to form holophosphatases which dephosphorylate a wide range of proteins in cells. ... There are more than 200 such phosphatases in humans that could in principle be studied using

4 Aug 2017: 1, A and B). Cross-linking reactionsof the active fractions revealed interacting part-ners of 200, 120, and 100 kD (Fig. ... The gradients were either 2 ml or 0.2 ml, on which either 200 µl or 20 µl of sample were layered.