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  2. David Komander to receive Young Investigator Programme EMBO Award -…

    https://www2.mrc-lmb.cam.ac.uk/david-komander-to-receive-young-investigator-programme-embo-award/
    Thumbnail for David Komander to receive Young Investigator Programme EMBO Award - MRC Laboratory of Molecular Biology 29 Nov 2010: David will join a vibrant network of 200 Young Investigators from all over Europe.
  3. Chapter 20 In Vitro Dissection of Protein Translocationinto the ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/62_Sharma_AS_MMB_2010.pdf
    30 Apr 2010: 4. 100 mM CaCl2: Dissolve in water. Keep at 4C.5. 200 mM EGTA. ... A good range to start is between 30and 200 µL of packed bead volume.
  4. JCB: Article The Rockefeller University Press $30.00J. Cell…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/63_Rane_NS_JCB_2010.pdf
    16 Feb 2010: JCB 6 of 12. Because Prl-PrP(AV3)11 develops disease at earlier ages than PrP(A117V)H mice ( 200 d versus 570 d), we compared their respective CtmPrP levels more
  5. A ribosome-associating factor chaperones tail-anchored membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/66_Mariappan_M_Nature_2010.pdf
    1 Aug 2010: The translation timefor RNC preparation was 15 min at 32 uC, after which the samples (volumetypically 200 ml) were chilled on ice and immediately layered on 2-ml 10–50%. ... Centrifugation was for 1 h at55,000 r.p.m. at 4 uC in a TLS-55 rotor (Beckman),
  6. Doc2b Is a High-Affinity Ca2+ Sensor forSpontaneous Neurotransmitter…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/GroffenScience2010.pdf
    30 Apr 2010: ST-D. oc2b. C2AB. 4AGS. T-Do. c2b. C2AB. K23. 7,319. E. 200 400 600 800 1000 12000. ... Neurosci. 25,. 5127 (2005).29. V. Chan-Palay, Z. Anat. Entwicklungsgesch. 134, 200.
  7. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovEHDs.pdf
    7 Apr 2010: FEBS Letters 584 (2010) 1830–1839. j o u r n a l h o m e p a g e : w w w. F E B S L e t t e r s. o r g. Review. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains. Felix Campelo a,b, Gur Fabrikant a, Harvey T. McMahon c,
  8. The Acetyltransferase Activity of the Bacterial Toxin YopJ ofYersinia …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Mittal_JBC2010.pdf
    4 Oct 2010: Bound material was elutedfrom the resin using 10 ml of 2 N hydrochloric acid, lyophilizedto remove the acid, and taken up in 200 l water. ... time in a 200-l volume using a FluoroMax-2spectrofluorometer (Horiba Yvon Spex).
  9. Bacterial actin MreB assembles in complex with cell shape protein RodZ

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf
    17 Feb 2010: Before theexperiments, proteins were dialysed to 20 mM Tris–HCl, pH 7.5,1 mM EDTA, 1 mM sodium azide, 200 mM NaCl. ... Tris–HCl, pH 9.0, 1 mMEDTA, 1 mM NaN3, 200 mM NaCl, 2 mM TCEP and prespun at138 000 g for 10 min, 41C.
  10. Features critical for membrane binding revealed by DivIVA crystal…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf
    20 May 2010: The EMBO Journal, (2010). doi:10.1038/emboj.2010.99
  11. A positively charged channel within the Smc1/Smc3 hinge required for…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf
    1 Dec 2010: Smc1DDSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200. 10.0. 5.01.00.0. ... 5.0. 1.00.0. Smc1DD E1158QSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200.

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