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  2. Features critical for membrane binding revealed by DivIVA crystal…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf
    20 May 2010: The EMBO Journal, (2010). doi:10.1038/emboj.2010.99
  3. 201010176 1..6

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Aylett-TubZ.pdf
    21 Oct 2010: All GTPγS-boundsubunits also retain a Mg2þ ion although only four of 11 GDP-bound subunits do so, despite crystallization with 200 mM MgCl2.When aspartate 64 in H2 is not
  4. David Komander to receive Young Investigator Programme EMBO Award -…

    https://www2.mrc-lmb.cam.ac.uk/david-komander-to-receive-young-investigator-programme-embo-award/
    Thumbnail for David Komander to receive Young Investigator Programme EMBO Award - MRC Laboratory of Molecular Biology 29 Nov 2010: David will join a vibrant network of 200 Young Investigators from all over Europe.
  5. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovEHDs.pdf
    7 Apr 2010: FEBS Letters 584 (2010) 1830–1839. j o u r n a l h o m e p a g e : w w w. F E B S L e t t e r s. o r g. Review. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains. Felix Campelo a,b, Gur Fabrikant a, Harvey T. McMahon c,
  6. Bacterial actin MreB assembles in complex with cell shape protein RodZ

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf
    17 Feb 2010: Before theexperiments, proteins were dialysed to 20 mM Tris–HCl, pH 7.5,1 mM EDTA, 1 mM sodium azide, 200 mM NaCl. ... Tris–HCl, pH 9.0, 1 mMEDTA, 1 mM NaN3, 200 mM NaCl, 2 mM TCEP and prespun at138 000 g for 10 min, 41C.
  7. A positively charged channel within the Smc1/Smc3 hinge required for…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf
    1 Dec 2010: Smc1DDSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200. 10.0. 5.01.00.0. ... 5.0. 1.00.0. Smc1DD E1158QSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200.
  8. MRC plate poster 18 8 2010 hr

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/Poster%20Plates.pdf
    6 Sep 2010:  reservoir holds 200 µl.  use robot or manually dispense 0.1 - 10 µl of reservoir and protein.
  9. Crystal Structures of Bacillus subtilis Lon Protease

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/londumanjmb2010.pdf
    20 Jul 2010: the protease domainof Lon is a 150- to 200-amino-acid-long, highlyconserved region located at the C terminus.
  10. JCB: Article The Rockefeller University Press $30.00J. Cell…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/63_Rane_NS_JCB_2010.pdf
    16 Feb 2010: JCB 6 of 12. Because Prl-PrP(AV3)11 develops disease at earlier ages than PrP(A117V)H mice ( 200 d versus 570 d), we compared their respective CtmPrP levels more
  11. A Large Particle Associated with the Perimeter of the ...

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb82.pdf
    5 Jul 2010: 3). Clearly visible are : (a) "rings" which have an insidediameter close to 800 A and an outer diameter the same as thatofthe pore complex itself(- 1,200 A) ; (b) large particles

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