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16 Feb 2010: JCB 6 of 12. Because Prl-PrP(AV3)11 develops disease at earlier ages than PrP(A117V)H mice ( 200 d versus 570 d), we compared their respective CtmPrP levels more

17 Feb 2010: Before theexperiments, proteins were dialysed to 20 mM Tris–HCl, pH 7.5,1 mM EDTA, 1 mM sodium azide, 200 mM NaCl. ... Tris–HCl, pH 9.0, 1 mMEDTA, 1 mM NaN3, 200 mM NaCl, 2 mM TCEP and prespun at138 000 g for 10 min, 41C.

7 Apr 2010: FEBS Letters 584 (2010) 1830–1839. j o u r n a l h o m e p a g e : w w w. F E B S L e t t e r s. o r g. Review. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains. Felix Campelo a,b, Gur Fabrikant a, Harvey T. McMahon c,

30 Apr 2010: 4. 100 mM CaCl2: Dissolve in water. Keep at 4C.5. 200 mM EGTA. ... A good range to start is between 30and 200 µL of packed bead volume.

30 Apr 2010: ST-D. oc2b. C2AB. 4AGS. T-Do. c2b. C2AB. K23. 7,319. E. 200 400 600 800 1000 12000. ... Neurosci. 25,. 5127 (2005).29. V. Chan-Palay, Z. Anat. Entwicklungsgesch. 134, 200.

20 May 2010: The EMBO Journal, (2010). doi:10.1038/emboj.2010.99

5 Jul 2010: 3). Clearly visible are : (a) "rings" which have an insidediameter close to 800 A and an outer diameter the same as thatofthe pore complex itself(- 1,200 A) ; (b) large particles

20 Jul 2010: the protease domainof Lon is a 150- to 200-amino-acid-long, highlyconserved region located at the C terminus.

1 Aug 2010: The translation timefor RNC preparation was 15 min at 32 uC, after which the samples (volumetypically 200 ml) were chilled on ice and immediately layered on 2-ml 10–50%. ... Centrifugation was for 1 h at55,000 r.p.m. at 4 uC in a TLS-55 rotor (Beckman),

2 Aug 2010: After a wash step with buffer A, the column was eluted using a gradient from 0–100% buffer B (buffer A 200 mM imidazole).