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30 May 2008: 0.5 M sucrose in PSB, 200 µL) by centrifugation at 100,000 rpm for 45 min at 4 C in a.

16 Jul 2008: Stable celllines were generated by selection in 200 g/ml Zeocin (Invitrogen, Carlsbad,CA) for 4 wk, followed by subcloning of individual colonies.

18 Sep 2008: Note that the death of some animals (in both trans-. genic lines) by 200 days is unrelated to the PrP transgenes orto neurodegenerative illnesses.

16 May 2008: 12, Vac1 and EEA1) and that binds to the membrane lipid phosphati-dylinositol-3-phosphate. Dense-core ... Double C2-like-domain-containing proteins. Synaptotagmin-like proteins. Multiple-C2-domain and transmembrane-domain proteins. 200 amino

13 Dec 2008: The Hydrophobic Insertion Mechanism of Membrane CurvatureGeneration by Proteins. Felix Campelo,y Harvey T. McMahon,z and Michael M. KozlovDepartment of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv,

28 Nov 2008: Note the protein-. dependent presence of tubular structures. The scale bar represents 200 nm. ... beads (Amersham Biosciences) and gel filtration on a sephacryl S-200 column (Amersham) as previously described [1].

11 Nov 2008: HeLa cells that had been serum-starved for 16 hours were stimulated with either EGF (20 ng/ml), TPA (200 ng/ml) or isoproterenol (10 µM) at 37ºC and the activation ... 60. 65. 70. 75. 200 300 400 500 600 700 800 900 1000 1100 12000.

6 Feb 2008: Depending on molecular weight of the protein and quantity of the protein a Sephadex 200 or 75 16/60 or 26/60 (Amersham Biosciences) was used. ... Proteins were transferred from polyacrylamide gels onto nitrocellulose (Protean) at a limiting current of

16 Aug 2008: tion plates mixing 200 200 nl of protein and crystallization solution. ... KOPS1 DNA. Crystallization solution was 200 mM citric acid/NaOH pH 3.5.

4 Aug 2008: Plasmid 43:200–204. ParMRC plasmid partitioningJ Salje and J Löwe. The EMBO Journal VOL 27 | NO 16 | 2008 & 2008 European Molecular Biology Organization2238.