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15 Dec 2008: Membranes were stained using 10 µg/mL FM-464 for ten minutes. 200 µL of cells were taken directly from.

13 Dec 2008: The Hydrophobic Insertion Mechanism of Membrane CurvatureGeneration by Proteins. Felix Campelo,y Harvey T. McMahon,z and Michael M. KozlovDepartment of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv,

28 Nov 2008: Note the protein-. dependent presence of tubular structures. The scale bar represents 200 nm. ... beads (Amersham Biosciences) and gel filtration on a sephacryl S-200 column (Amersham) as previously described [1].

11 Nov 2008: HeLa cells that had been serum-starved for 16 hours were stimulated with either EGF (20 ng/ml), TPA (200 ng/ml) or isoproterenol (10 µM) at 37ºC and the activation ... 60. 65. 70. 75. 200 300 400 500 600 700 800 900 1000 1100 12000.

16 Oct 2008: More than 200 analogs of these hits wereiteratively synthesized through diversification of the R1 and R2substituents (Supplementary Table 1). ... We profiled PP121 and PP487against over 200 protein kinases and found that they inhibit a patternof tyrosine

4 Mar 2008: coli FtsQ and L. monocytogenes FtsQ, in20 mM Tris, 1 mM EDTA, 1 mM NaAzide, 200 mM NaCl,pH 7.0 (TEN200) for Y.

30 May 2008: 0.5 M sucrose in PSB, 200 µL) by centrifugation at 100,000 rpm for 45 min at 4 C in a.

16 Jul 2008: Stable celllines were generated by selection in 200 g/ml Zeocin (Invitrogen, Carlsbad,CA) for 4 wk, followed by subcloning of individual colonies.

16 May 2008: 12, Vac1 and EEA1) and that binds to the membrane lipid phosphati-dylinositol-3-phosphate. Dense-core ... Double C2-like-domain-containing proteins. Synaptotagmin-like proteins. Multiple-C2-domain and transmembrane-domain proteins. 200 amino

3 Sep 2008: pH 7.5], 200 mM NaCl, 2 mM b-mercaptoethanol) and finally buffer C again. ... ments were cut as 200 3 400 pixel boxes at 1.25 Å/pixel with a 369 pixel over-.