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120 L fermentor: Quick guide
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWfermentor/runfermentor.html27 Feb 2006: Press "Activate". Switch on stirrer to dissolve powder (Control: Ag(itation) speed=100-200 rpm). ... Stirrer 100-200, oxygen enrichment on (sp=0), air 60-100 (watch out for too much foam); NOTE: DO should be OFF. -
1202.tif
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb84.pdf16 Jun 2006: 5) , andthe i r w i dth rema i ned fa i r l y constant (800 - 1 , 200 A). -
Acetylation of MEK2 and I{kappa}B kinase (IKK) activation loop…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJ.pdf28 Nov 2006: Acetyl Transferase Assays. GST-MEK2 (0.5 g) (purified by usingstandard protocols for GST-tagged protein purification) wasincubated with varying amounts (0 –200 ng) of (His)6-YopJ-wt ... Stimulation of MEK and Erk phosphorylation by various -
doi:10.1016/j.cell.2006.01.047
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16615893.pdf18 Apr 2006: D) Gel filtration of full-length ESCRT-I, ESCRT-I (D21-Vps37), and ESCRT-I core on a Superdex 200 HR10/30. ... mM Tris [pH 7.5], 200 mM NaCl and 5 mM b-mercaptoethanol). -
doi:10.1016/j.cell.2006.03.035
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16647110.pdf14 Jun 2006: 200-fold more potently than p110b. p110a inhibitors. also blocked activation of the mTORC1 pathway as mea-. -
doi:10.1016/j.cell.2006.10.032
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/43_Kang_SW_Cell_2006.pdf30 Nov 2006: Substrate-Specific TranslocationalAttenuation during ER Stress Definesa Pre-Emptive Quality Control PathwaySang-Wook Kang,1,4 Neena S. Rane,1,4 Soo Jung Kim,2 Jennifer L. Garrison,3 Jack Taunton,3. and Ramanujan S. Hegde1,1 Cell Biology and -
doi:10.1016/j.jbbm.2005.12.008
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/rfcloning2006%20published.pdf19 Apr 2006: Miroux personal communication), 200 AM dNTP’s (Roche), 1!reaction buffer,2.5 units PfuTurbok (Stratagene) in a total volume of 50 Al. ... TCF1 200 3–6 2.0 4.6 4/4 OK Yes nd. TCF2 200 3–6 2.0 4.6 4/4 OK Yes nd. -
doi:10.1016/j.molcel.2006.06.019
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Ftsk%20mc%202006.pdf12 Aug 2006: agent (200 ml in 700 ml water) was added and Pi release monitored. ... tios of these proteins were mixed (in buffer A plus 200 mM NaCl;. -
doi:10.1016/j.molcel.2006.07.020
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/HiroseMC2006.pdf8 Sep 2006: cold stage in a Philips Tecnai F20 electron microscope operating. at 200 kV. -
doi:10.1016/S0076-6879(05)04052-8
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/HigginsMcMahonMethods2005.pdf26 Jan 2006: A lossof 100–200 l is usually observed. Purification of Dynamin and Its Mutants from E. ... Scale bars are 200 nm in. length. [52] IN VITRO RECONSTITUTION OF ENDOCYTIC STAGES 607.
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