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12 Nov 2006: Briefly, a 1 ml sample of each PX domain was loaded onto a Superdex 200 column (Amersham-Pharmacia) equilibrated and eluted with 20 mM Tris-HCl buffer, pH 7.4, containing

12 Nov 2006: Further purifi cation was by gel fi ltration on Superdex 200 in 10 mM Tris-Cl, pH 8.0, 0.15 M NaCl, 1 mM DTT, and 1 mM EGTA and

14 Jun 2006: 200-fold more potently than p110b. p110a inhibitors. also blocked activation of the mTORC1 pathway as mea-.

18 Apr 2006: D) Gel filtration of full-length ESCRT-I, ESCRT-I (D21-Vps37), and ESCRT-I core on a Superdex 200 HR10/30. ... mM Tris [pH 7.5], 200 mM NaCl and 5 mM b-mercaptoethanol).

16 Jun 2006: 5) , andthe i r w i dth rema i ned fa i r l y constant (800 - 1 , 200 A).

27 Feb 2006: Press "Activate". Switch on stirrer to dissolve powder (Control: Ag(itation) speed=100-200 rpm). ... Stirrer 100-200, oxygen enrichment on (sp=0), air 60-100 (watch out for too much foam); NOTE: DO should be OFF.

19 Apr 2006: Miroux personal communication), 200 AM dNTP’s (Roche), 1!reaction buffer,2.5 units PfuTurbok (Stratagene) in a total volume of 50 Al. ... TCF1 200 3–6 2.0 4.6 4/4 OK Yes nd. TCF2 200 3–6 2.0 4.6 4/4 OK Yes nd.

12 Oct 2006: c) Superdex 200 gel filtration of E. coli FtsKg in the presence of DNA. ... Storage buffer was. 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA and 1 mM sodium azide.

28 Sep 2006: 50 nm. 100 nm. 200 nm. I. Supplementary Figure 2. The crystal structure of BDLP in GDP-associated and nucleotide-free conformation.

7 Dec 2006: A pre-culture, grown in 2¥ TY, was used in a1:1000 dilution to inoculate 200 ml of minimal medium con-taining 1¥ M9 supplemented with 0.4% glucose, 2 mMMgSO4,