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Contrasting Membrane Interaction Mechanisms of AP180N-terminal…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Stahelin_cho2003.pdf16 Jul 2003: The membraneassociation of peripheral proteins can be accelerated by longrange electrostatic interactions (24), and the larger ka for theENTH domain is consistent with its more positive electrostaticpotential, especially around the ... smaller ka) but -
cdf519 5057..5068
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12356722.pdf12 Oct 2002: Resolution (AÊ ) 2.0 2.0Observations/unique reections 83 922/24 307 84 280/24 356Completeness (last shell) % 93.6 (74.1) 93.7 (74.1)Rmerge. ... Phosphoinositide ka (M1 s1) kd (s. 1) Kd (M) Fold increase in Kda. -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: replacing the leoA̧ leoB and leoAB genes with a chloramphenicolresistance cassette as described [24]. ... 24. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes. -
pnas201205508 15259..15264
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22949682.pdf25 Nov 2012: B) Protein–lipid FRET assays were per-formed with 5% PIP2 vesicles, and Ka was calculated for both 5 μM pY. ... JBiol Chem 283:31227–31236. 24. Burke JE, et al. (2008) Interaction of group IA phospholipase A2 with metal ions andphospholipid vesicles -
Supplemental Materials Supp MethodsSupp Fig. 1 Sequence conservation…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/AP2Hub_EMBO2004/AP2Praefcke2004Sup.pdf23 Nov 2004: Peptide binding to a-adaptin wild-type appendagePeptide Sequence Kd(mM) [Peptide/. appendage]Ka(M-1) DH(kcal. ... Eps15-P1 SFGDGFADFSTL 127.0 24. 0.9 0.1 8.03e3 1.4e3. -9.7 3.0 -5.2 3.1. -
PII: S1097-2765(02)00515-4
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf19 Apr 2002: tide linker had little or no effect on its ability to bindstate affinity measurements to estimate on rate (ka), offrate (kd), and affinity (KA ka/kd) constants by measuring Scc1 (Figure ... dissociation rate constants (ka and kd, respectively) are -
Structure and superorganization of acetylcholinereceptor–rapsyn…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf3 Jul 2013: 20%of the receptors showed restricted diffusion in small domains of50 nm, whereas the majority of the receptors were immobile (24).Confocal microscopy of overexpressed rapsyn in nonmuscle. ... The cross-linking of receptors by rapsyn,therefore, seems to -
doi:10.1016/j.neuron.2005.05.004
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Jockusch_Neuron2005.pdf17 Jun 2005: 223 40 pA; 5M GDP-β-s and 0.5 mM ATP, 83 9 fF and 173 24 pA. ... 2004). Monitoring clathrin-mediated endocytosis during synaptic activity. WmJ. Neurosci. 24, 2004–2012.nNeves, G., and Lagnado, L. -
Cryo‐EM structure of the full‐length Lon protease from Thermus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf14 Nov 2021: 24]. All subsequent image-processing steps were performed. using single-particle reconstruction methods in RELION. ... aerolysin family protein. Nat Commun 7, 11293. 23 Zheng Shawn Q, Palovcak E, Armache J-P, Verba KA,. -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: coli FtsKN used in the in vivo experiments, 24 amino acids forthe E. ... Mol. Microbiol.27:257–268. 24. Steiner W, Liu G, Donachie WD, Kuempel P. -
Reconstitution of a prokaryotic minus end-trackingsystem using TubRC…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2015-Fink-E1845-50.pdf27 Apr 2015: ProcNatl Acad Sci USA 80(16):4922–4925. 24. Howard J, Hudspeth AJ, Vale RD (1989) Movement of microtubules by single kinesinmolecules. ... J Bacteriol 196(24):4304–4314. 30. Westermann S, et al. (2006) The Dam1 kinetochore ring complex moves -
bic296a
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/EberthBJ4170371.pdf24 Jan 2009: Binding parameters such asnumber of binding sites (n), the association constant (Ka) and thebinding enthalpy (H) were determined as parameters ofthe fitted experimental binding values. ... J. Biol. Chem.280, 6716–6720. 24 Longenecker, K. L., Zhang, B. L -
CELL AND MOLECULAR PHYSIOLOGY Intracellular Ca2+ release via the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/44_Ong_HL_Pflug_Arch_2007.pdf15 Feb 2007: Measurements weredone after 24 h. [Ca2+]i measurements. Measurements were conducted as described previously[19]. ... 24. Paschen W, Doutheil J, Gissel C, Treiman M (1996) Depletion ofneuronal endoplasmic reticulum calcium stores by thapsigargin:effect on -
doi:10.1016/j.biocel.2008.08.010
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cytomotive2008Lowe.pdf17 Sep 2008: Bacterial DNA segregation dynamicsmediated by the polymerizing protein ParF. EMBO J 2005;24:1453–64. ... Coexistence of tubulins andftsZ in different Prosthecobacter species. Mol Biol Evol 2007;24:1439–42. -
740 Research Paper Calcium triggers calcineurin-dependent synaptic…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Marks_McMahon1998.pdf21 Mar 2003: Correspondence: Harvey T. McMahonE-mail: hmm@mrc-lmb.cam.ac.uk. Received: 3 March 1998Revised: 24 April 1998Accepted: 5 May 1998. ... synapse. Cell 1994, 79:717-727. 24. DeBello WM, Betz H, Augustine GJ: Synaptotagmin andneurotransmitter release. -
Membrane Localization is Critical for Activation of the PICK1 BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf18 Oct 2008: C-terminal 24 residues downstream from the putative. internalization motif in the DAT C-terminus to Tac. ... Of interest, a recent study has suggested a lipid-binding. capacity also for the PDZ domain (24). -
16001658465475 1..36
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Collier_2020_elife-59560-v1.pdf15 Sep 2020: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 33. Received: -
30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf1 May 2004: 2004.Structural/functional homology betweenthe bacterial and eukaryotic cytoskele-tons.Curr. Opin. Cell Biol.16:24–31. 3. ... Cell Biol.13:55–60. 36. Erickson HP, Taylor DW, Taylor KA,Bramhill D. 1996. -
2077 741..748
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/21_Lingappa_VR_BioEssays_2002.pdf5 Jul 2002: Cell 1986;47:711– 719. 24. Liao S, Lin J, Do H, Johnson AE. ... Dill KA, Chan HS. From Levinthal to pathways to funnels. Nat Struc Biol. -
The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf28 Nov 2008: Titration curves were fitted to the data using the ORIGIN program (MicroCal Inc.) which yielded the stoichiometry (1), the binary association constant Ka (=Kd -1) and the enthalpy of binding. ... The entropy of binding (S) was calculated from the
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