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4 Nov 2011: the endogenous control (actin 5C). The viral titre or geneexpression in resistant flies relative to susceptible flies was.

18 Nov 2015: 2015; Walker et al. 2015).Furthermore, a number of arthropod genomes contain inte-grated endogenous viral elements (EVEs) with similarity torhabdoviruses, suggesting that these species have been infectedwith rhabdoviruses at some ... intothe insect

18 Mar 2015: The viral RNA load was expressedrelative to the endogenous control housekeeping gene RpL32 (Rp49). ... Two qRT-PCR reactions(technical replicates) were carried out per sample with both the viral and endogenous controlprimers.

16 Jan 2014: an endogenous control reference gene (Elongation factor 1a 100E(EF1a100E)) using a QuantiTect Virus qRT-PCR kit (Qiagen,UK) and Taqman hydrolysis probes (Sigma-Aldrich, UK) on a. ... Relative viral RNA copy. number was then calculated relative to the

10 Aug 2012: UNCORRECTED PROOF Date: 12:15 Thursday 21 June 2012File: Rhabdoviruses 3P. 8The Sigma Viruses of DrosophilaBen Longdon, Lena Wilfert and Francis M. Jiggins. AbstractThe sigma virus of Drosophila melanogaster (DMelSV) was discovered accidentally over

26 Sep 2011: The copy-number of viral genomic RNA was expressed. relative to the endogenous control housekeeping gene RpL32 (Rp49).We designed different RpL32 primers specific for each species. ... technical replicates) were carried out per sample with both the.

18 Jul 2011: J. 2010 Endogenous viralelements in animal genomes. PLOS Genet. 6,e1001191. (doi:10.1371/Journal.Pgen.1001191).

11 May 2011: RNA was extractedand reverse-transcribed (see above), and qRT-PCR was used tomeasure the viral titer relative to an endogenous control(RpL32) using the delta delta CT method.

22 Apr 2021: RESEARCH ARTICLE. Widespread conservation and lineage-specificdiversification of genome-wide DNAmethylation patterns across arthropodsSamuel H. Lewis1,2,3, Laura RossID. 4, Stevie A. BainID4, Eleni PahitaID. 2,3, StephenA. SmithID. 5, Richard

5 Dec 2017: Virus identification and genome assembly. To identify viruses, we first mapped RNA-Seq reads to the genome of the host species to exclude genome-derived transcripts, thus filtering out endogenous viral ... Nat. Commun. 7, 13856 (2016). 9. Ghildiyal, M.