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  2. Department of Engineering - Contacting people from web pages

    www3.eng.cam.ac.uk/contact_addresses.html
    Contacting people from web pages. The default maintainer of any individual page should be shown at the bottom of the page, though the actual maintainer of the information may be mentioned earlier. You can contact them following the guidelines below.
  3. Milner Therapeutics Institute Newsletter

    www2.gurdon.cam.ac.uk/~milnernews/Back%20Issues/December%2020.html
    26 Feb 2021: CRISPR in Drug Discovery 2021. 23-24 March, Online. This meeting will focus on the application of genome engineering to identify novel drug targets through large scale CRISPR based functional genomics
  4. Publications | Ahringer Lab

    www2.gurdon.cam.ac.uk/~ahringerlab/publications.html
    16 Nov 2016: Extreme HOT regions are CpG dense promoters in C. elegans and human Genome Research 24(7):1138-46 | PDF. ... EMBO Journal, 24(51): 2613-2623.| PDF.
  5. Comparative assessment of fluorescent proteins for in vivo imaging in …

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2016_Mol-Biol-Cell-2016-Heppert-3385-94.pdf
    15 Nov 2016: Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res 24, 142–153. ... Genome Res 24, 1138–1146. Cranfill PJ, Sell BR, Baird MA, Allen JR, Lavagnino Z, de Gruiter HM, Kremers G-J, Davidson MW,
  6. genesdev255810 495..500

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2015_Genes_Dev_Latorre-495-500.pdf
    15 Nov 2016: EMBO J 24: 2613–. 2623. Raj A, van den Bogaard P, Rifkin SA, van Oudenaarden A, Tyagi S.
  7. Comparative analysis of metazoan chromatin organization

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2014_Nature13415.pdf
    15 Nov 2016: Brent Chen5, Ron A.-J. Chen11,Daniel S. Day1,24, Yan Dong11, Andrea C. ... 24. Egelhofer, T. A. et al. An assessment of histone-modification antibody quality.Nature Struct.
  8. JCB_201310083 1..16

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2014_JCB_201310083.pdf
    15 Nov 2016: 100% after 24 h, confirming that sld-2 is an essential gene in C. ... L1 worms fed with the indicated RNAi were incubated at 20C until 24 and 36 h after they reached the adult stage.
  9. genome161992 1138..1146

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2014_Genome-Res-1138.pdf
    15 Nov 2016: 1138 Genome Researchwww.genome.org. 24:1138–1146 Published by Cold Spring Harbor Laboratory Press; ISSN 1088-9051/14; www.genome.org. ... 2011. Integrative genomics viewer. Nat Biotechnol 29: 24–26. Schug J, Schuller WP, Kappen C, Salbaum JM, Bucan M,
  10. genesdev238105 783..796

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2014_Genes-Dev-Weick-783-96.pdf
    15 Nov 2016: In Drosphila and mammals, piRNAs are pro-duced from long precursor transcripts, which subse-quently undergo processing to mature piRNA sequenceswith a length distribution of 24–32 nucleotides (nt) anda strong
  11. pgen.1003679 1..18

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2013_PLoSGenetics2.pdf
    6 Jan 2014: MRE-11. and RAD-50 are also required for DSB formation [24,25] as in S. ... which are required for DSB formation in C. elegans [24,25]. In htp-3.
  12. pgen.1003330 1..12

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2013_PLoSGenetics.pdf
    9 Jul 2013: Fitness w1,2 lowerthan expected is interpreted as evidence of masking [18,19,24]. ... 100. Plates were incubated at 15uC for 6 days, when controls hadbeen laying eggs for ,24 hrs.
  13. Systematic genetic interaction screens uncover cell polarity…

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2013_NatureCellBiology.pdf
    25 Mar 2013: R E S O U R C E. Systematic genetic interaction screens uncover cellpolarity regulators and functional redundancyBruno Thomas Fievet1,5, Josana Rodriguez1,5, Sundar Naganathan2, Christine Lee1, Eva Zeiser1, Takao Ishidate3,Masaki Shirayama3, Stephan
  14. genome153668 1339..1347

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2013_GenomeResearch.pdf
    6 Jan 2014: 10.1101/gr.153668.112Access the most recent version at doi:2013 23: 1339-1347 originally published online April 2, 2013Genome Res. Ron A.-J. Chen, Thomas A. Down, Przemyslaw Stempor, et al. reveals promoter and enhancer architectureselegansC.The
  15. pgen.1002933 1..13

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2012_PlosGenetics.pdf
    25 Mar 2013: and trimethylation of H4K20 [22,23,24]. The C. elegans orthologsof these proteins are SET-1 (PR-Set7/SETD8) and SET-4 (Suv4-. ... produced in the next 24 hours of RNAi feeding, or if RNAi is.
  16. gkr425 1..9

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2011_NucleicAcidsRes.pdf
    25 Mar 2013: These sequences were mappedonto the genome using MAQ (24) in the same way that wetreated experimental sequence data (i.e. ... elegans genomeusing MAQ (24) in the same way that we treated experi-mental sequence data.
  17. genome115519 1..10

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2011_GenomeRes.pdf
    8 Mar 2011: 10.1101/gr.115519.110Access the most recent version at doi: published online December 22, 2010Genome Res. Tao Liu, Andreas Rechtsteiner, Thea A. Egelhofer, et al. elegansC.Broad chromosomal domains of histone modification patterns in.
  18. DOI: 10.1126/science.1196914, 1775 (2010);330 Science , et al.Mark B. …

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2010_Science_330_1775.pdf
    8 Mar 2011: www.sciencemag.org SCIENCE VOL 330 24 DECEMBER 2010 1777. RESEARCH ARTICLES. on. ... S22A). www.sciencemag.org SCIENCE VOL 330 24 DECEMBER 2010 1779. RESEARCH ARTICLES.
  19. doi:10.1016/j.cell.2010.05.011

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2010_Cell.pdf
    21 Dec 2010: Leading Edge. Review. Cell 141, May 28, 2010 2010 Elsevier Inc. 757. IntroductionMost animal cells display obvious polarity, indicating the presence of molecular asymmetries. The general steps of cell polarity, including its establishment and
  20. The caenorhabditis elegans CDT-2 ubiquitin ligase is required for…

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2010_BMC_Dev_Biol.pdf
    21 Dec 2010: genotypes % Persistent exp. N % Persistent exp. N. cul-4/cul-4 62.5 24 75 8. ... Embo J 2005, 24:2613-23. 18. Yoo AS, Bais C, Greenwald I: Crosstalk between the EGFR and LIN-12/Notch pathways in C.
  21. npgrj_ng_322 376..381

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2009_NatGen_41_376.pdf
    27 Apr 2009: There is evidence that one function ofH3K36me3 in the gene body is to prevent aberrant transcriptioninitiation23,24. ... H3K4me3. H3K36me3. ba. c. d. e. 3. –23. –23. –24. –24.
  22. RNAi has become a widely used method for studying ...

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2008_NatRevGenet_9_554.pdf
    5 Jan 2009: RNAi in Drosophila cell culture is relatively easy because dsRNA molecules are simply added to cultured cells and incubated for 24–96 hours (depending on the assay and cell type) before
  23. TH EJ OU RN AL OF CE LL BIO ...

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2008_JCellBiol_180_877.pdf
    5 Jan 2009: Oncogene. 24 : 2844 – 2859. van Vugt , M.A. , B.C.
  24. doi:10.1016/j.devcel.2008.06.002

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2008_DevCell_15_198.pdf
    5 Jan 2009: incubated for 27 and 24 hr for ppk-1(RNAi) and csnk-1(RNAi), respectively.
  25. pone.0000493 1..5

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2007_PLoSOne_2_e493.pdf
    12 Jun 2007: 1/T10G3.5 would target GFP to early endosomes in C. eleganssomatic tissues [24]. ... J Cell. Biol 103: 2241–2252.24. Roggo L, Bernard V, Kovacs AL, Rose AM, Savoy F, et al.
  26. doi:10.1016/j.ydbio.2007.02.022

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2007_DevBiol_305_347.pdf
    4 Sep 2007: After the first incubation adults were transferred to freshplates and allowed to lay eggs for 24 h at 20 C before removal. ... Where two ACs are separated by more than oneVPC width (21 μm, n=55), then most animals displaymultiple, non-adjacent 1 fates
  27. 1471-213X-7-19.fm

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2007_BMCDevBiol_20_7_19.pdf
    4 Sep 2007: Shown are plates incubated at 25C for 36 hours and at 37C for 24 hours. ... Development 2003, 130(8):1605-1620. 18. Yeong FM: Anaphase-promoting complex in Caenorhabditiselegans. Mol Cell Biol 2004, 24(6):2215-2225.
  28. Chromatin regulation and sumoylation in theinhibition of Ras-induced…

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2005_EMBOJ_24_2613.pdf
    20 Jul 2005: 2005 European Molecular Biology Organization The EMBO Journal VOL 24 | NO 14 | 2005. ... 62; 13) 3.21 (33; 12)F29B9.6 ubc-9 3.0 (0; 20) 3.24 (24; 17) 5.13 (100; 8)W01G7.3 — 3.0 (0; 18) ND, let
  29. PII: S1534-5807(04)00066-8

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2004_DevCell_6_511.pdf
    26 Aug 2004: GFP-expressing lines were. grown at 24 C, and all other strains were grown at 20 C. ... After an additional 24 hr,How might SPD-2 function in both centrosome matu-these plates were examined and live and dead progeny counted.ration and duplication?
  30. plbi-01-01-15 77..84

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_PLoS_1_E12.pdf
    16 Dec 2003: 64 58 60IV 61 66 29 34 24 31V 49 50 28 23 24 19X 64 64 32 27 25 25Total 397 405 244 232 213 209Percentage 100% 100% 61% 57% ... 24(s71), CB3497 dpy-25(e817), MT1222 egl-6(n592), MT1179 egl-14(n549), MT1067 egl-31(n472), MT151 egl-33(n151), MT171
  31. 61865 238..294

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_Nature_421_268.pdf
    18 Dec 2003: Nature Genet 15, 397–401 (1997). 24. Apfeld, J. & Kenyon, C.
  32. 59477 231..237

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_Nature_421_231.pdf
    18 Dec 2003: Nature Genet. 24, 180–183. (2000). 10. Piano, F., Schetter, A. J., Mangone, M., Stein, L. & ... Science 293, 2087–2092. (2001). 24. Lercher, M. J., Urrutia, A. O. &
  33. doi:10.1016/S1046-2023(03)00050-1

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_Methods_30_313.pdf
    16 Dec 2003: Ghosh, F.S. Collins,. Biotechniques 21 (1996) 700–709. [24] C.T. Chung, S.L. ... Dorovkov, A. Ryazanov,. M. Driscoll, Nat. Genet. 24 (2000) 180–183. [30] T.
  34. RESEARCH COMMUNICATION Identification of genesthat protect the C.…

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_GeneDev_17_443.pdf
    16 Dec 2003: elegans against muta-tions. (2) For 24 genes, we determined the mutation fre-quency using an endogenous monitor gene (Greenwaldand Horvitz 1980; see also below). ... elegans germline. Thisassay was performed for 24 genes (Y-axis), several from each
  35. doi:10.1016/S0960-9822(03)00577-3

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_CurrBiol_13_1499.pdf
    16 Dec 2003: Centrosomal TACCtics. Bioessays 24,. 915–925.Acknowledgments 17. Gergely, F., Kidd, D., Jeffers, K., Wakefield, J.G., and Raff, J.W. ... 24. Wittmann, T., Hyman, A., and Desai, A. (2001). The spindle: acentrosomal proteins that can interact with
  36. doi:10.1016/S0960-9822(03)00539-6

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_CurrBiol_13_1311.pdf
    16 Dec 2003: silencing complex) in Drosophila [23], Neurospora [24], silencing and cosuppression in C. ... Science 293, 1146–1150.quence of the nematode C. elegans: a platform for investigating 24.
  37. doi:10.1016/S0960-9822(03)00371-3

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_CurrBiol_13_1029.pdf
    16 Dec 2003: This work waswere fixed at least 24 hr after mothers were injected with dsRNA. ... 2000). A role for RIC-8 (Synembryn). 24. Dechant, R., and Glotzer, M.
  38. doi:10.1016/S0955-0674(02)00018-2

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_COCB_15_73.pdf
    16 Dec 2003: 3 ' I E I $ # B >/ 2 3 ) 0 7 ' ' -996 $ /3;24 3;-<. < &
  39. PII: S0092-8674(03)00985-1

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_Cell_115_825.pdf
    12 Jan 2004: elegans ORF ZC8.4 in the Received: January 24, 2003coiled-coil region. Revised: November 4, 2003. ... Accepted: November 22, 2003Published: December 24, 2003Generation of GFP Reporters and Two-Hybrid Constructs.
  40. 1471-213X-3-8.fm

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2003_BMCDB_3_8.pdf
    16 Dec 2003: These phenotypes and the similarityof POD-1 to coronin, a gene involved in endocytosis andphagocytosis [23,24], suggest polarization of the zygotemay involve membrane trafficking processes. ... ye11); 42% symmetric, n = 24 for emb-8(hc69); and29%
  41. dophila was determined to be 61 fs (11).The carotenoid ...

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2002_Science_298_2398.pdf
    16 Dec 2003: tochem. Photobiol. 73, 219 (2001).24. In order to explain the red shift of PAx with respect to. ... 54, 503 (1993).23. S. J. Lin et al., Nature 418, 344 (2002).24.
  42. vab-7 specifies motoneurone identity

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2002_Dev_129_853.pdf
    16 Dec 2003: Wild type 89% (144/162) 0% (0/135) 27vab-7(e1562) 99% (143/144) 88% (105/120) 24.
  43. PII: S0960-9822(02)01041-2

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2002_CurrBiol_12_1317.pdf
    16 Dec 2003: Received: May 24, 2002Revised: June 12, 2002Accepted: June 13, 2002Published: August 6, 2002.
  44. cn063a

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2001_NatCB_3_297.pdf
    18 Dec 2003: embryonic lethality. Differential interference contrast 4D video microscopy.Embryos from injected mothers were analysed 24–48 h after injection.
  45. http://genomebiology.com/2000/2/1/research/0002.1 com ment reviews…

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2001_GenmBiol_2_r2.pdf
    18 Dec 2003: expressing gfp dsRNA for (b)24 h, (c) 48 h, (d) 72 h, or (e) 96 h. ... After being fed for 24 h,GFP expression was markedly reduced (b) compared tosimilarly treated unfed worms (a).
  46. cde420 3984..3992

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2001_EMBOJ_20_3984.pdf
    16 Dec 2003: Injected worms were kept at 22C for 24 h prior todissection of embryos. ... After completion of recordings, slides were kept at 15C for 24 hprior to collecting a single series of 24 focal planes for analysis of theterminal phenotype.
  47. PII: S0960-9822(01)00142-7

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2001_CurrBiol_11_482.pdf
    18 Dec 2003: elegans CDC-42 protein [24], goat was supported by a Wellcome Senior Research Fellowship to J.A., an HFSPanti-Cdc42 and rabbit anti-Cdc42 raised against S. ... a Rho family gtpase activatingprotein (GAP) required for central spindle formation andWhen
  48. PII: S0959-437X(00)00206-9

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2001_COGD_11_367.pdf
    18 Dec 2003: 42 might activate the PAR-3–PAR-6–PKC-3 complex [23•,24•]. ... elegans. Curr Biol 2001,. 11:482-488.See annotation [24• ]. 24. Kay AJ, Hunter CP: CDC-42 regulates PAR protein localisation and • function to control cellular and embryonic
  49. PII: S0168-9525(00)02066-7

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2000_TIG_16_351.pdf
    18 Dec 2003: Trends Cell Biol. 9,193–198. 24 Jones, P.L. et al. (1998) Methylated DNA and MeCP2 recruithistone deacetylase to repress transcription. ... Sci. 24, 142–145. 45 Brehm, A. et al. (1998) Retinoblastoma protein recruits.
  50. N408_16c 325..330

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/2000_Nature_408_325.pdf
    18 Dec 2003: Phenotypic analysis of lethality/sterility was carried out at t = 24 hand post-embryonic phenotypes were analysed by two independent observers at t = 36, 48,60 and 72 h. ... Mol. Biol. 215, 403410 (1990). 24. Barnes, T. M., Kohara, Y., Coulson, A. &
  51. Global embryonic patterning in C. elegans

    www2.gurdon.cam.ac.uk/~ahringerlab/papers/1999_Dev_126_2483.pdf
    16 Dec 2003: For comparison, in a cross of we3 unc-4(e120) withwild-type males, 24/144 were Mor, 0/80 non-unc hermaphroditeswere Dyf and 0/30 males were Mtl. ... Injectedanimals were singled on plates for 12 hours, then transferred to newplates every 24 hours.

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