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Cell, Vol. 85, 217–228, April 19, 1996, Copyright 1996 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/5_Hegde_RS_Cell_1996.pdf25 Apr 1997: Furthermore, because the epi-and its entry into the translocation channel, whereas a. ... Although the length of time that the nents, demonstrated the requirement for a surprisinglychain is exposed to the cytosol is brief, it is significant. -
16262551036386 1..32
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cohesin%20folding%202021%20elife-67268-v1.pdf14 Jul 2021: Shi et al., 2020; Higashi et al., 2020) and does so in the absence of Scc3 without entry inside the. ... chore protein Ctf19, a requirement that is apparently not bypassed by smc1D588Y. -
Cellular/Molecular Endophilin Drives the Fast Mode of Vesicle…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Llobet%20J%20Neurosci2011.pdf23 Sep 2011: All data are expressed as mean SEM Entries in bold are significantly different from the other conditions using a one-way ANOVA followed by Dunnett’s multiple-comparisons test (p ... J Neurosci Methods76:1–5. Sato K, Ernstrom GG, Watanabe S, Weimer RM, -
Substrate-driven assembly of a translocon for multipass membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Nature_2022b.pdf19 Aug 2023: The ER also contains members of the Oxa1 superfamily of TMD insertases9, including the guided entry of tail anchored protein (GET) complex10, the ER membrane protein complex (EMC)11 and TMCO1 ... 2f and Extended Data Fig. 4b). This is consistent with the -
The Function, Structure, and Origins of the ER Membrane Protein…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Annual_Rev_Biochem_2022.pdf11 Apr 2022: investigation of tail-anchored (TA)membrane protein insertion by the GET (guided entry of TA protein) pathway provided anunexpected insight that ultimately led to the discovery of a key EMC function. ... Although GET3and EMC2 are unrelated proteins, they -
Molecular Biology of the CellVol. 19, 3463–3476, August 2008 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/51_Ashok_A_MBC_2008.pdf16 Jul 2008: Molecular Biology of the CellVol. 19, 3463–3476, August 2008. Retrotranslocation of Prion Proteins from the EndoplasmicReticulum by Preventing GPI Signal TransamidationAarthi Ashok and Ramanujan S. Hegde. Cell Biology and Metabolism Program, -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/227
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/227{"id":227,"date":"2012-06-25T12:46:57","date_gmt":"2012-06-25T11:46:29","guid":{"rendered":"http://www2.mrc-lmb.cam.ac.uk/wordpress/?page_id=227"},"modified":"2024-04-04T16:31:10","modified_gmt":"2024-04-04T15:31:10","slug":"student-placement-scheme" -
Membrane Protein Insertion at the Endoplasmic Reticulum
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/75_Shao_S_Ann_Rev_2011.pdf9 Nov 2011: This strongly disfavors its translocation into the lumen, a requirement for sampling the type Itopology. -
Protein targeting and degradation are coupled for elimination of…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/73_Hessa_T_Nature_2011.pdf14 Jul 2011: 2e andSupplementary Fig. 13), correlating with the requirements for ubiqui-tination (Fig. ... 4f). Ribosomessynthesizing nascent membrane proteins can recruit both the SRPand Bag6 complex on entry of the first hydrophobic segment intothe ribosomal tunnel4 -
doi:10.1016/j.devcel.2005.09.001
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/37_Shaffer_KL_DevCell_2005.pdf7 Oct 2005: 2002), different requirements for ac-cessory translocon components such as TRAM (Voigtet al., 1996) or the TRAP complex (Fons et al., 2003),and the choice of translocation pathway taken by thesubstrate ... In fact, since the signal sequences of Crtnd -
JCB: Article The Rockefeller University Press $30.00J. Cell Biol. ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/J%20Cell%20Biol%202010%20Howes.pdf24 Aug 2011: 6 F). To test the requirement for CLIC endocytosis during mi-gration, this inactivation assay was applied to migrating fibro-blasts. -
Dbouk 1..13
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/vadas_science_signaling.pdf5 Dec 2012: The inhibitoryactivity of the myristoylated peptide required its entry into the cells be-cause both myristoylated and TAT-tagged peptides inhibited Gbg-dependentactivation of Akt, whereas unmodified peptide had no such ... With regard to the requirement -
, 130081, published 7 August 20133 2013 Open Biol. ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Open%20Biol.-2013-TakedaCombined.pdf25 Sep 2013: 130081, published 7 August 20133 2013 Open Biol. Harvey T. McMahon and David M. GloverTetsuya Takeda, Iain M. Robinson, Matthew M. Savoian, John R. Griffiths, Anthony D. Whetton, the plasma membrane and contractile ring in cytokinesis. F-BAR protein -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/24
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/24{"id":24,"date":"2012-06-25T09:57:52","date_gmt":"2012-06-25T09:57:52","guid":{"rendered":"http://www2.mrc-lmb.cam.ac.uk/wordpress/?page_id=24"},"modified":"2023-07-21T12:49:26","modified_gmt":"2023-07-21T11:49:26","slug":"manuscripts-correspondence" -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/20739
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/20739 -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/12771
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/12771However we are mindful that the extent of research opportunities can vary a lot as do the length undergraduate courses, master requirements, and all these are put into context when evaluating ... nnnn. If you have heard of us, spread the word or enrol -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/19288
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/19288 -
Manuscripts & Correspondence - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/about-lmb/archive/manuscripts-correspondence/21 Jul 2023: Gonville and Caius. July 1953. Bound photocopy. Miscellaneous collection of Crick material has been collected by Mark Bretscher – see entry under BRETSCHER, Mark. ... Dissertations submitted for the degree of Doctor of Philosophy in the University of
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Key Dates for Applicants - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/students/international-phd-programme/key-dates-for-applicants/6 Sep 2023: 22nd December 2023 – 3rd January 2024. LMB Postgraduate Office Christmas closure.
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Overview of admissions - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/students/international-phd-programme/overview-of-admissions/12 Sep 2023: However we are mindful that the extent of research opportunities can vary a lot as do the length undergraduate courses, master requirements, and all these are put into context when evaluating ... If you have heard of us, spread the word or enrol for the
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Electron Cryo-microscopy - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/news-events/public-engagement/cryo-electron-microscopy/feed/3 Jul 2024: Our goal was to understand the mechanisms of viral entry, fusion, and structural maturation./p pWhy did you change focus to work on COVID-linked research?/p p style="padding-left: -
HARVEY T McMAHON CV
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/people/CV2008c.htm28 Jul 2008: I participate in lectures to postgraduate students both within our own institute and in other MRC institutes. ... 2+. entry. Neuron 6, 517-524 (1991). 11. McMahon, H.T. and Nicholls, D.G. -
The Coot User Manual
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/docs/coot.html1 Mar 2021: Hardware Requirements:. • Environment Variables:. • Command Line Arguments:. • Web Page:. • -
Professional registration and awards: Celebrating the Technician’s…
https://www2.mrc-lmb.cam.ac.uk/professional-registration-and-awards-celebrating-the-technicians-commitment-at-the-lmb/3 Dec 2021: Leading the way if you like. Since passing the competency report as part of the registered technician entry requirement, I have become a Member of the Institute of Science and Technology ... The hardest part was relating what I did in my current job role
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Contents:
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/balbes/documentation_fs/manual_115.html31 Jan 2012: We update and release the internal database of BALBES for homologous searching every month to include entries related to new structures released by Protein Data Bank(PDB). ... The requirements depend on what you want to do with BALBES. -
Testing the capacity for intracellular antibodies to neutralise…
https://www2.mrc-lmb.cam.ac.uk/testing-the-capacity-for-intracellular-antibodies-to-neutralise-sars-cov-2/29 Jul 2021: Standard assays for antibody neutralisation examine the ability of antibodies to directly block the entry of a virus into a cell. ... Although this assay is not suitable for population screening due to its requirement for CL3 lab space and use of live
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Molecular basis for membrane remodelling and organization
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/F-BAR_proteins/JMConference/speakerprofiles.html21 Nov 2010: Cell. 2008 Mar 7;132(5):807-17. A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. ... Traffic. 2007 Oct;8(10):1385-403. Entry mechanisms for toxins and viruses, and the role of membrane curvature. -
ProSHADE: ProSHADE_internal::ProSHADE_distances Class Reference
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d1/d6f/class_pro_s_h_a_d_e__internal_1_1_pro_s_h_a_d_e__distances.html1 Nov 2018: It returns a vector of vectors of energy level distances from the fragment to all passing database entries. ... It returns a vector of vectors of trace sigma distances from the fragment to all passing database entries. -
30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf1 May 2004: have similar, almost exchangeable functions, asdemonstrated recently by a gain-of-function point mutation in FtsA that removesthe requirement for ZipA completely (45). ... maritima(Figure 10). The protein was found to polymerizeeasily under mild -
PII: S0014-5793(01)02216-5
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinD%20FEBS.pdf5 Mar 2001: 3. Results. Structurally MinD closely resembles other nucleotide bind-ing proteins such as nitrogenase iron proteins and GTPasessuch as Ffh (PBD entry 1NG1 [20]), part of the signal recog-nition particle ... The nucleotide binding surface of MinD is the -
Opening of LMB Containment Level 3 lab fast-tracked to help fight the …
https://www2.mrc-lmb.cam.ac.uk/opening-of-lmb-containment-level-3-lab-fast-tracked-to-help-fight-the-covid-19-pandemic/23 Oct 2020: sure the laboratory met all legal requirements. ... Leo: In the context of COVID-19, everyone is looking at the spike protein – which coats the outside of the virus particle and mediates cell entry – and how spike antibodies stop
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Cryo‐EM structure of the full‐length Lon protease from Thermus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf14 Nov 2021: collar, may facilitate substrate entry and possibly also. help with translocation into the AAA+ channel. ... tral channel, which possibly facilitates the entry of cer-. tain substrates (and itself). -
Bacterial chromosome segregation: structure andDNA binding of the Soj …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/soj_embo2005.pdf30 Jan 2005: confirming that ATP-dependent dimerisation is the critical. requirement for assembly of the protein onto DNA (Figure. ... domains dimerise, a requirement for HTH-mediated DNA. binding (Leonard et al, 2004). -
PII: S1388-1981(99)00150-X
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10570253.pdf20 Apr 2002: from PLC-N1 (pdb entry code 1MAI) was determined independently of the structure of theremainder of the enzyme (pdb entry code 2ISD). ... Sequence analysis suggests that. Fig. 7. The C2 domain. The C2 domains from PLC-N1 and PKCL (pdb entry code 1A25) are -
Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: superposition on the DNA complex of phage 343 repressor. (PDB entry 2or1; Aggarwal et al, 1988). ... Colour code as in (B). Double-stranded DNA in grey (taken from pdb entry 2OR1). -
Filament structure and subcellular organization of the bacterial…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Liu_PNAS_crescentin_2024.pdf28 Apr 2024: Functional Requirements. Having determined the assembly and subcellular organization of CreS, we next addressed the molecular determinants of CreS- dependent cell curvature generation. ... Nevertheless, correct CreS assembly is not the only functional -
Molecular mechanism and physiological functions of clathrin-mediated…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/McMahon%20NRMB%202011.pdf19 Jul 2011: In this case, the clathrin lattice may serve as a signalling platform for actin polymerization, which may thus make it indispen sible for cellular entry of these very large cargoes. ... The pathway is also exploited by toxins, viruses and bacteria to -
ProSHADE: src/proshade/ProSHADE_saveLoad.cpp Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d0/df1/_pro_s_h_a_d_e__save_load_8cpp_source.html1 Nov 2018: ProSHADE 0.6.5 (NOV 2018). Protein Shape Descriptors and Symmetry Detection. ProSHADE_saveLoad.cpp. Go to the documentation of this file.. 1. 21 //============================================ RVAPI. 22 #include <rvapi_interface.h>. 23. 24 //========= -
Dynamic Filaments of the Bacterial Cytoskeleton
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2006.pdf19 Apr 2006: arrow). (center,top) Note the closed conformation (PDB entry 1MWM) and (bottom) the open conformation of the apoand ADP-bound forms of ParM, both from E. ... right) AMPPNP-bound FtsA from T. maritima (PDB entry 1E4G)(136). www.annualreviews.org • -
mmi_3991.fm
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ftsn2004.pdf15 Apr 2004: The requirement of FtsN for cell division in. E. coli. became obvious with a conditional null allele of the. ... Residues A127-L132apparently contribute a fourth helix, the Table entries forwhich are italicized here because they involve non-nativeresidues -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: chromosomal region from ETEC H10407 genome and confirmed. the NCBI entry NC_017633.1 (Figure S1 in File S1). ... The presence of LeoA within the bacterial periplasm and its. potential requirement for hetero-dimerisation with LeoBC for. -
Structural and Mechanistic Comparison of Prokaryotic and Eukaryotic…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9466937.pdf15 Jan 1998: Results and Discussion. The PI-PLC fold. The crystal structures of PI-PLC from Bacilluscereus (bPI-PLC; PDB entry code 1PTD) and PI-. ... entry ofphospholipid head groups into the active site orthe release of product following catalysis. -
The structure of human thyroglobulin
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf31 Jan 2020: To validate our list of requirements for hormonogenesis from poly-peptide chains, we set out to engineer synthetic T4 hormonogenic sites into the unrelated bacterial MBP. ... 4. Cahnmann, H. J., Pommier, J. & Nunez, J. Spatial requirement for coupling of -
ANRV378-BI78-31.tex
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DohertyMcMahonAnnRevBiochem09.pdf27 Apr 2009: often artificially clustered) endogenous mark-ers or bacterial exotoxins predominantly by flu-orescence microscopy, from determining thelipid requirements for these pathways, from ex-amining their sensitivity to certain drugs, andfrom ascertaining -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: All residues in the RsaA249–1026 X-ray structure are well ordered,reflecting a requirement that the S-layer lattice must avoid flexibleloops, as they would be easy targets for hostile ... Despite forming a con-tinuous sheet, there is a requirement for -
mmi_4133.fm
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Spo0J%202004.pdf30 Jun 2004: maintained (for example, the RAP1 protein fam-ily, PDB entry 1IGN; Konig et al., 1996). ... 2. The secondary structure of KorB–C (PDB entry 1IGQ) is shown below the alignment. -
PII: S0166-2236(03)00131-0
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/25_Hegde_RS_TINS_2003.pdf30 Jun 2003: neurodegeneration [1]. Yet, all heritable and transmissibleprion diseases seem to share certain characteristicpathological changes and an obligate requirement forPrP expression, suggesting at least some common aspectsto their pathogenesis. -
nature14896 493..505
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/95_Brown_A_Nature_2015.pdf19 Aug 2015: In the structures here, the essentialNIKS sequence (residues 61–64)21, located at the end of helix a2,imposes a requirement for uridine in the first (11) position via inter-actions ... Purines would be disfavoured at the 11 position by steric -
Protein-driven membrane stresses in fusion and fission
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovCurvature2010.pdf21 Feb 2011: Proteins as energy generators for membraneremodelingMembrane remodeling, either by fusion or fission, canoccur if two physical requirements are fulfilled. ... Thereare two important requirements for this mechanism ofmembrane fusion. First, the -
TH EJ OU RN AL OF CE LL BIO ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/53_Hegde_RS_JCB_2008.pdf22 Jul 2008: When posi-tioned in the looped orientation, elongation of the nascent chain results in its entry into the ER lumen. ... The only requirement would be a capacity to interact, at least weakly, with specifi c regions of a nascent chain to bias its
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