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  2. Understanding TRIM21 activation allows Trim-Away toolbox expansion -…

    https://www2.mrc-lmb.cam.ac.uk/understanding-trim21-activation-allows-trim-away-toolbox-expansion/
    Thumbnail for Understanding TRIM21 activation allows Trim-Away toolbox expansion - MRC Laboratory of Molecular Biology 9 Mar 2021: In fact, this may explain why TRIM21 evolved this requirement for clustering as anything entering the cell coated with multiple antibodies must require a quick response and degradation.
  3. ProSHADE: ProSHADE_internal::ProSHADE_distances Class Reference

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d1/d6f/class_pro_s_h_a_d_e__internal_1_1_pro_s_h_a_d_e__distances.html
    1 Nov 2018: It returns a vector of vectors of energy level distances from the fragment to all passing database entries. ... It returns a vector of vectors of trace sigma distances from the fragment to all passing database entries.
  4. 30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf
    1 May 2004: have similar, almost exchangeable functions, asdemonstrated recently by a gain-of-function point mutation in FtsA that removesthe requirement for ZipA completely (45). ... maritima(Figure 10). The protein was found to polymerizeeasily under mild
  5. Opening of LMB Containment Level 3 lab fast-tracked to help fight the …

    https://www2.mrc-lmb.cam.ac.uk/opening-of-lmb-containment-level-3-lab-fast-tracked-to-help-fight-the-covid-19-pandemic/
    Thumbnail for Opening of LMB Containment Level 3 lab fast-tracked to help fight the COVID-19 pandemic - MRC Laboratory of Molecular Biology 23 Oct 2020: sure the laboratory met all legal requirements. ... Leo: In the context of COVID-19, everyone is looking at the spike protein – which coats the outside of the virus particle and mediates cell entry – and how spike antibodies stop
  6. PII: S0014-5793(01)02216-5

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinD%20FEBS.pdf
    5 Mar 2001: 3. Results. Structurally MinD closely resembles other nucleotide bind-ing proteins such as nitrogenase iron proteins and GTPasessuch as Ffh (PBD entry 1NG1 [20]), part of the signal recog-nition particle ... The nucleotide binding surface of MinD is the
  7. Cryo‐EM structure of the full‐length Lon protease from Thermus…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf
    14 Nov 2021: collar, may facilitate substrate entry and possibly also. help with translocation into the AAA+ channel. ... tral channel, which possibly facilitates the entry of cer-. tain substrates (and itself).
  8. Bacterial chromosome segregation: structure andDNA binding of the Soj …

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/soj_embo2005.pdf
    30 Jan 2005: confirming that ATP-dependent dimerisation is the critical. requirement for assembly of the protein onto DNA (Figure. ... domains dimerise, a requirement for HTH-mediated DNA. binding (Leonard et al, 2004).
  9. Filament structure and subcellular organization of the bacterial…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Liu_PNAS_crescentin_2024.pdf
    28 Apr 2024: Functional Requirements. Having determined the assembly and subcellular organization of CreS, we next addressed the molecular determinants of CreS- dependent cell curvature generation. ... Nevertheless, correct CreS assembly is not the only functional
  10. Bacterial actin MreB assembles in complex with cell shape protein RodZ

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf
    17 Feb 2010: superposition on the DNA complex of phage 343 repressor. (PDB entry 2or1; Aggarwal et al, 1988). ... Colour code as in (B). Double-stranded DNA in grey (taken from pdb entry 2OR1).
  11. PII: S1388-1981(99)00150-X

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10570253.pdf
    20 Apr 2002: from PLC-N1 (pdb entry code 1MAI) was determined independently of the structure of theremainder of the enzyme (pdb entry code 2ISD). ... Sequence analysis suggests that. Fig. 7. The C2 domain. The C2 domains from PLC-N1 and PKCL (pdb entry code 1A25) are
  12. Dynamic Filaments of the Bacterial Cytoskeleton

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2006.pdf
    19 Apr 2006: arrow). (center,top) Note the closed conformation (PDB entry 1MWM) and (bottom) the open conformation of the apoand ADP-bound forms of ParM, both from E. ... right) AMPPNP-bound FtsA from T. maritima (PDB entry 1E4G)(136). www.annualreviews.org •
  13. mmi_3991.fm

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ftsn2004.pdf
    15 Apr 2004: The requirement of FtsN for cell division in. E. coli. became obvious with a conditional null allele of the. ... Residues A127-L132apparently contribute a fourth helix, the Table entries forwhich are italicized here because they involve non-nativeresidues
  14. pone.0107211 1..10

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf
    3 Sep 2014: chromosomal region from ETEC H10407 genome and confirmed. the NCBI entry NC_017633.1 (Figure S1 in File S1). ... The presence of LeoA within the bacterial periplasm and its. potential requirement for hetero-dimerisation with LeoBC for.
  15. The structure of human thyroglobulin

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf
    31 Jan 2020: To validate our list of requirements for hormonogenesis from poly-peptide chains, we set out to engineer synthetic T4 hormonogenic sites into the unrelated bacterial MBP. ... 4. Cahnmann, H. J., Pommier, J. & Nunez, J. Spatial requirement for coupling of
  16. Molecular mechanism and physiological functions of clathrin-mediated…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/McMahon%20NRMB%202011.pdf
    19 Jul 2011: In this case, the clathrin lattice may serve as a signalling platform for actin polymerization, which may thus make it indispen sible for cellular entry of these very large cargoes. ... The pathway is also exploited by toxins, viruses and bacteria to
  17. ProSHADE: src/proshade/ProSHADE_saveLoad.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d0/df1/_pro_s_h_a_d_e__save_load_8cpp_source.html
    1 Nov 2018: ProSHADE 0.6.5 (NOV 2018). Protein Shape Descriptors and Symmetry Detection. ProSHADE_saveLoad.cpp. Go to the documentation of this file.. 1. 21 //============================================ RVAPI. 22 #include <rvapi_interface.h>. 23. 24 //=========
  18. mmi_4133.fm

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Spo0J%202004.pdf
    30 Jun 2004: maintained (for example, the RAP1 protein fam-ily, PDB entry 1IGN; Konig et al., 1996). ... 2. The secondary structure of KorB–C (PDB entry 1IGQ) is shown below the alignment.
  19. Structure of the hexagonal surface layer on Caulobacter crescentus…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf
    6 Apr 2017: All residues in the RsaA249–1026 X-ray structure are well ordered,reflecting a requirement that the S-layer lattice must avoid flexibleloops, as they would be easy targets for hostile ... Despite forming a con-tinuous sheet, there is a requirement for
  20. PII: S0166-2236(03)00131-0

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/25_Hegde_RS_TINS_2003.pdf
    30 Jun 2003: neurodegeneration [1]. Yet, all heritable and transmissibleprion diseases seem to share certain characteristicpathological changes and an obligate requirement forPrP expression, suggesting at least some common aspectsto their pathogenesis.
  21. Structural and Mechanistic Comparison of Prokaryotic and Eukaryotic…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9466937.pdf
    15 Jan 1998: Results and Discussion. The PI-PLC fold. The crystal structures of PI-PLC from Bacilluscereus (bPI-PLC; PDB entry code 1PTD) and PI-. ... entry ofphospholipid head groups into the active site orthe release of product following catalysis.
  22. ANRV378-BI78-31.tex

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DohertyMcMahonAnnRevBiochem09.pdf
    27 Apr 2009: often artificially clustered) endogenous mark-ers or bacterial exotoxins predominantly by flu-orescence microscopy, from determining thelipid requirements for these pathways, from ex-amining their sensitivity to certain drugs, andfrom ascertaining
  23. ProSHADE: src/proshade/ProSHADE_internal.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d9/dc4/_pro_s_h_a_d_e__internal_8cpp_source.html
    1 Nov 2018: 818 if ( all->size() < 1 ). 819 {. 820 std::cerr << "! ProSHADE ERROR! Error in file comparison! The compare against part has not enough entries." << std::endl;. ... 883 if ( all->size() < 1 ). 884 {. 885 std::cerr << "! ProSHADE ERROR! Error in file
  24. Protein-driven membrane stresses in fusion and fission

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovCurvature2010.pdf
    21 Feb 2011: Proteins as energy generators for membraneremodelingMembrane remodeling, either by fusion or fission, canoccur if two physical requirements are fulfilled. ... Thereare two important requirements for this mechanism ofmembrane fusion. First, the
  25. nature14896 493..505

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/95_Brown_A_Nature_2015.pdf
    19 Aug 2015: In the structures here, the essentialNIKS sequence (residues 61–64)21, located at the end of helix a2,imposes a requirement for uridine in the first (11) position via inter-actions ... Purines would be disfavoured at the 11 position by steric
  26. Qrb1300006 1..40

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf
    30 Jul 2013: signal much more difficult to achieve. At the time the distortion–correction method was being developed, the symmetrical, top-entry. ... tional drift-stability compared with standard side-entry cold stages, and the overall microscope.
  27. Thesis Chapter 0

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Hardaker.pdf
    10 Jun 2008: elegans. A thesis submitted in partial satisfaction of the. requirements for the degree Master of Science.
  28. Amphiphysin is necessaryfor organization of the…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DroxAmph.pdf
    9 Nov 2001: Nevertheless, becauseamphmut larvae clearly show a much milder phenotypethan that of shibirets larvae at their restrictive tempera-ture, amphiphysincannotbeanabsolute requirement forsynaptic vesicle recycling at the NMJ.The amph mutants also showed
  29. 3747_0_supp_7_1052456676

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/christian.pdf
    9 May 2003: Cameleon imaging of calcium transients in cultured mechanosensory neurons in Caenorhabditis elegans. Christian Frøkjær-Jensen Speciale. February 2003 Niels Bohr Institute. University of Copenhagen. 2. Table of contents Abstract. 3 Credit. 4
  30. TH EJ OU RN AL OF CE LL BIO ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/53_Hegde_RS_JCB_2008.pdf
    22 Jul 2008: When posi-tioned in the looped orientation, elongation of the nascent chain results in its entry into the ER lumen. ... The only requirement would be a capacity to interact, at least weakly, with specifi c regions of a nascent chain to bias its
  31. Dissertation

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/AndyHuang.pdf
    6 Mar 2008: A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy.
  32. Refined Structure of (alpha)(beta)-Tubulin at 3.5 A Resolution

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/jmb%20tubulin%20refinement.pdf
    3 Nov 2001: Anders andD. Botstein, personal communication), in furtheragreement with the requirement of these residuesfor nucleotide hydrolysis. ... Initial amplitudes were obtainedfrom 94 electron diffraction patterns, which had an over-all Friedel R-factor of 19 %
  33. articles Nucleotide-dependent conformational changes in dynamin:…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Stowell_McMahon1999.pdf
    30 Apr 1999: articles. entry into nerves is stimulated by the depolarization of nerve termi-nals, the phosphatase calcineurin is activated and amphiphysin anddynamin are dephosphorylated, potentially allowing them tointeract13. ... 28. Steinweis, P. C. & Gilman, A. G.
  34. Abstract_Book_HMM_v2

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/conferences/JM_Abstract_Book.pdf
    15 Sep 2011: In an attempt to identify the upstream activator of Rac1 in IL-2R entry, we tested the role of p85α and PI 3- Kinase. ... could lead to the recruitment and/or the activation of Rac1 in the clathrin- and caveolin-independent entry used by IL-2R uptake. ==
  35. Membrane fusion is the process whereby two separate lipid ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MartensMcMahon2008.pdf
    16 May 2008: Further-more, enveloped viruses gain entry into the cytosol by fusing their limiting membranes with host cell membranes (FIG. ... Analogous to the requirement of HOpS for efficient vacuole fusion. in yeast, the tethering factor and rab5 effector eeA1 is
  36. Tumour prevention by a single antibody domaintargeting the…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17568777.pdf
    22 Jul 2007: LTR, long terminal repeat; FLAG, antibody tag; IRES, internalribosome entry site; SP, signal peptide (the sequences shown are for the nls or memb.). ... Proc Natl Acad SciUSA 93: 6665–6670. Rangarajan A, Hong SJ, Gifford A, Weinberg RA (2004) Species-
  37. Quality and quantity control at the endoplasmic reticulum

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/65_Hegde_RS_COCB_2010.pdf
    7 Jun 2010: Given the ability of certain proteins to insert in,and possibly traverse the lipid bilayer spontaneously, withno essential requirement for membrane proteins demon-strated [34], alternatives to protein-conducting channelsshould probably ... 20! Bernasconi
  38. 01Eichler(Hedge)

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/33_Hegde_RS_Chapter_2005.pdf
    31 Oct 2005: By contrast, translocation into the ER (open arrow) is often regarded as a constitutive process wherethe presence of a signal sequence in a protein predetermines its entry into the ER. ... Thus, the entry of proteins into the ER is not necessarilya
  39. JFCregg_OGS_finaledit

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Cregg_thesis.pdf
    14 Dec 2010: Caenorhabditis Elegans. A dissertation submitted in partial satisfaction of the. requirements for the degree Doctor of Philosophy. ... entry’ to the UK for five months. Dr. Arnivan Ghosh for his ability as a committee.
  40. N402_18f 313..320

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10580505.pdf
    11 Nov 1999: protein kinase Lck30 (PDB entry 3lck). c, Stereo diagram of PI3Kg active site with bound. ... Different structural requirements within the switch II region of the Ras protein for.
  41. FtsA forms actin-like protofilaments

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276a.pdf
    27 Mar 2012: the requirement for ZipA, which is another Z-ring membrane. anchor in E.
  42. Membrane transport between compartments in eukary-otic cells requires …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynaminreview2004.pdf
    2 Feb 2004: Membrane transport between compartments in eukary-otic cells requires proteins that allow the budding andSCISSION of nascent cargo vesicles from one compartmentand their targeting and fusion with another. Just asSNARES are proposed to be essential
  43. doi:10.1016/j.cell.2006.10.032

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/43_Kang_SW_Cell_2006.pdf
    30 Nov 2006: such diversity in sequence, efficiency, or complexity in. their requirements for additional factors. ... that acts to minimize PrP entry into and misfolding within. the ER lumen.
  44. The mechanisms of integral membrane protein biogenesis

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/NRMCB_2021.pdf
    22 Sep 2021: The minimum requirement for SRP recognition is a predominantly hydrophobic sequence of approximately seven amino acids. ... e | Tail- anchored (TA) protein insertion by the guided entry of TA protein (GET) pathway.
  45. Target Selection during Protein Quality Control

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/96_Shao_S_TIBS_2016.pdf
    18 Feb 2016: In yeast, Sgt2 clients can be trans-ferred to the TRC40 homolog Get3 (guided entry of tail-anchored proteins 3) via the Get4–Get5bridging complex, with Get5 recruiting Sgt2 and Get4 ... This dual requirement wouldsharpen client discrimination by
  46. doi:10.1016/j.molcel.2004.08.004

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15350214.pdf
    24 Sep 2004: The observedsubstrate inhibition by Ins(1,4,5)P3 and non-linear kinetics with ATP(data not shown) may be caused by a requirement for ATP to bindbefore
  47. coot-user-manual.dvi

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/docs/coot-user-manual.pdf
    28 Sep 2012: 11.2 What is Coot? 11.3 What Coot is Not. 11.4 Hardware Requirements. ... 1.4 Hardware Requirements. The code is designed to be portable to any Unix-like operating system.

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