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A Coot for Cryo-EM: 0.9 (pre-release) ● ~1500 revisions ...
https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/CCP4-wg2-whats-new-in-Coot-2019.pdf28 May 2019: A Coot for Cryo-EM: 0.9 (pre-release). 1500 revisions ahead of the master branch This new branch is actively developed Considerations for the CootVR interface. ... Ignore it. – new Coot on Mac OS X and Windows doesn't matter. -
Model-Building with Modern Coot Paul Emsley Scripps, Feb 2019 ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Coot-Scripps-Feb-2019-EOS.pdf21 Feb 2019: Less stable More interesting Not always clear how to use the new functions. ... or that the new functions are there). A New Branch. New multi-threaded sections: Refinement. – -
Activation of GCN2 by the ribosomal P-stalk
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/PNAS_2019.pdf7 Mar 2019: New insight into a possible functional link be-tween GCN2 and ribosomes came from a recent analysis of micelacking both a specific neuronal tRNA (tRNAArgUCU) and theputative ribosome recycling factor GTPBP2 -
Current Developments in Coot for Macromolecular Model Building…
https://www2.mrc-lmb.cam.ac.uk/coot/protein-science/Casanal_Lohkamp_Emsley_11112019-pe-2.pdf11 Nov 2019: Faster refinement goes hand in hand with the addition of new restraint types. ... library. The new mode of Acedrg allows the generation of bespoke chemical links,. -
The structure of bactofilin filaments reveals their mode of membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf26 Aug 2019: from genomic DNA and cloned into vector pET15b using Gibson assembly (New England Biolabs). ... plasmid pHis17 using Gibson assembly (New England Biolabs) with a stop codon before the C-terminal tag on the plasmid. -
Mechanism of ribosome stalling during translation of a poly(A) tail
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/NSMB_2019.pdf25 Nov 2019: R., Mohr, S. C. & Smith, T. F. Genomic detection of new yeast pre-mRNA 3’-end-processing signals. -
Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Szewczak-Harris_et_al-2019-FEBS_Letters.pdf22 Jul 2019: form a new class of alternating, copolymeric filaments,. which can be assembled in vitro either on lipid mem-. ... Hagen WJ, Lindahl E and Scheres SH (2018) New. tools for automated high-resolution cryo-EM structure. -
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https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf29 May 2019: the presence of 100 mg/ml cycloheximide to suppress new protein synthesis. ... tion attenuation during acute ER stress, new GFP-PrP synthesis during the time course is relatively. -
Recognition and Degradation of Mislocalized Proteins in Health and…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CSHL_Persp_2019.pdf8 Mar 2019: 3). First, the load of new proteinsentering stressed mitochondria is reduced at theexpense of generating mislocalized proteins thatmust be degraded by cytosolic proteasomes(Wright et al. -
Biallelic Variants in ASNA1, Encoding a Cytosolic Targeting Factor of …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CircGen_2019.pdf1 Dec 2019: TA proteins in early embryonic development.11–16 This study offers the first evidence for its role in human disease, and provides new insight into the molecular mechanisms in DCM.
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