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  2. Hundreds of children and adults enjoy the LMB’s ‘See Your Cells’…

    https://www2.mrc-lmb.cam.ac.uk/hundreds-children-adults-enjoy-lmbs-see-cells-activity-cambridge-science-festival/
    Thumbnail for Hundreds of children and adults enjoy the LMB’s ‘See Your Cells’ activity at the Cambridge Science Festival - MRC Laboratory of Molecular Biology 23 Mar 2017: The first weekend of the 2017 Cambridge Science Festival saw over 3000 visitors attend the 'Hands-on at the Guildhall' event, where the LMB’s 'See Your
  3. Finding the founding fathers of molecular biology – a blog post by…

    https://www2.mrc-lmb.cam.ac.uk/finding-founding-fathers-molecular-biology-blog-post-lmb-phd-student-lisa-strittmatter/
    Thumbnail for Finding the founding fathers of molecular biology – a blog post by LMB PhD student, Lisa Strittmatter - MRC Laboratory of Molecular Biology 6 Dec 2017: Lisa Strittmatter, PhD student in the LMB’s Structural Studies Division, explores a recent talk given at the LMB by Professor Sir John Meurig Thomas, and
  4. G-protein coupled receptors as drug targets: Heptares Therapeutics…

    https://www2.mrc-lmb.cam.ac.uk/g-protein-coupled-receptors-drug-targets-heptares-therapeutics-celebrates-tenth-anniversary/
    Thumbnail for G-protein coupled receptors as drug targets: Heptares Therapeutics celebrates its tenth anniversary - MRC Laboratory of Molecular Biology 6 Jul 2017: Chris Tate – co-founder of Heptares Therapeutics, a company established to commercialise research from the LMB – discusses his work on G-protein coupled
  5. Scientists establish a comprehensive protein interactions map of the…

    https://www2.mrc-lmb.cam.ac.uk/scientists-establish-comprehensive-protein-interactions-map-replication-machinery-chronic-virus/
    Thumbnail for Scientists establish a comprehensive protein interactions map of the replication machinery of a chronic virus - MRC Laboratory of Molecular Biology 21 Dec 2017: Chronic viral infections are amongst the biggest threats to human health worldwide. Leo James’ group from the LMB’s PNAC Division, in collaboration with
  6. LMB scientist, Glenn Masson, spends a week shadowing MP for…

    https://www2.mrc-lmb.cam.ac.uk/lmb-scientist-glenn-mason-spends-week-shadowing-mp-cambridge-daniel-zeichner-royal-societys-pairing-scheme/
    Thumbnail for LMB scientist, Glenn Masson, spends a week shadowing MP for Cambridge, Daniel Zeichner, through the Royal Society’s Pairing Scheme - MRC Laboratory of Molecular Biology 13 Dec 2017: Glenn Masson has spent a week shadowing Daniel Zeichner, MP for Cambridge, through the Royal Society’s Pairing Scheme, an initiative which pairs
  7. Pymol guide

    https://www2.mrc-lmb.cam.ac.uk/groups/nagai/download/pymolguide.pdf
    19 Dec 2017: So if you click on (sele) you can toggle and untoggle your new selection. ... So if you want to make a new selection, make sure you deselect everything first!
  8. 6. Image refinement Sjors Scheres EM course 2017 Agenda ...

    https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-06-refinement/?wpdmdl=18760&refresh=668ff5b2bbbd31720710578
    4 Oct 2017: calculate new 2D average as probability weighted averages. Reference-free 2D class averaging. ... The bad news • The experimental data alone is not enough to determine a unique solu7on! •
  9. Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf
    29 May 2017: Added into a new N-linked tree: – paucimannose. 23/40. Xyl-β1,2. Xyl - β1,2 – Man – using XYP (beta D xylosepyranose). – ... both GUI interface and built into the auto-builder. New Interface.
  10. Structure of the hexagonal surface layer on Caulobacter crescentus…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf
    6 Apr 2017: 3), requiring RsaA to adoptslightly different conformations. Furthermore, gaps in the latticewill be needed for insertion of new RsaA molecules on growingcells. ... The new culture was grown at 30 C for2–3 days. The culture was imaged using a
  11. ligand-fitting-tutorial.dvi

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ligand-fitting-tutorial.pdf
    24 Oct 2017: The centre of the screen is a bit crowded now. Undisplay those new ligands. ... When we are happy with our fit, we can merge this new ligand into the mainprotein ligand as we did before.
  12. Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf
    29 Dec 2017: dejongeii DSM 12251 (PdBtubAB), a stop codon (TAA)was introduced before the C-terminal hexahistidine tag in the BtubAB bacterialT7 expression plasmid used (7) by Q5 mutagenesis (New England Biolabs). ... E5954 |
  13. Fei Long MRC Laboratory of Molecular Biology, Cambridge, U.K. ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Ligand_meeting_Sept_29_2017.pdf
    29 Sep 2017: new_atom_id _chem_mod_atom.new_type_symbol _chem_mod_atom.new_type_energy _chem_mod_atom.new_charge TMPmod1 delete H2. ... chem_mod_bond.new_type _chem_mod_bond.new_value_dist _chem_mod_bond.new_value_dist_esd TMPmod1 delete C1 H2 single.
  14. 1. A historical perspective and course overview

    https://www2.mrc-lmb.cam.ac.uk/download/image-formation-fourier-analysis-and-ctf-theory-paula-da-fonseca/?wpdmdl=18758&refresh=668ff5b2825221720710578
    30 Jun 2017: Carter, C.B. Transmission electron microscopy (Plenum Press, New York, 1996). •
  15. REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf
    4 Aug 2017: These constructs are in a vector (provided with the PURE system kit from New England Biolabs) that contains a T7 promoter and transcriptional terminator. ... The resin was washed four times with 1 ml PSB2 and transferred to a new tube after the fourth
  16. A Polymerization-Associated Structural Switch in FtsZ That Enables…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf
    3 May 2017: the divisome around the division site (17, 18).These findings have resurrected an old model of bacterial cell division: the templatemodel, in which the closing septum constricts by new cell wall ... because the energetic cost of losing subunits from ends
  17. IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf
    13 Jan 2017: using the Gibson Assembly kit (New England Biolabs).
  18. REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf
    19 Jan 2017: PURE system translation reactions. Initial experiments used the PURE translation system from New England Biolabs as per the manufacturer’s instructions.
  19. doi:10.1016/j.pbiomolbio.2004.07.009

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf
    22 Sep 2017: authors. Thesedevelopments along with advances in structure determination techniques (mainly the use ofsynchrotrons, area detectors and crystal freezing) led to an enormous increase in new proteinstructures: in 1990, about 500 structures

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