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Using cryo-EM to solve the structure of a protein complex critical…
https://www2.mrc-lmb.cam.ac.uk/using-cryo-em-solve-structure-protein-complex-critical-gene-expression/7 Nov 2017: A Log-in or subscription may be required to view this article from the host site: https://www.drugtargetreview.com/news/27088/cryo-electron-microscopy-cpf/]. -
Network control principles predict neuron function in the C. elegans…
https://www2.mrc-lmb.cam.ac.uk/network-control-principles-predict-neuron-function-c-elegans-connectome/19 Oct 2017: This work provides new and powerful theoretical tools to allow us to interpret these brain maps and understand the how the nervous system works at the most fundamental, cellular level. -
Wojciech Galej receives Early Career Research Award from the…
https://www2.mrc-lmb.cam.ac.uk/wojciech-galej-receives-early-career-research-award-biochemical-society/11 Apr 2017: Wojciech Galej has been awarded the 2018 Early Career Research Award for Molecular Structure and Function by the Biochemical Society. Wojciech has made -
1 JED: Questions Arising ● Does the Acedrg new ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/JED-questions-arising.pdf30 Sep 2017: i.e., use the same description and apply it to the new ligand in question, just like for sugar links. ... generated by Acedrg's new mode). We don't have anything new to offer for modifications. -
Richard Henderson and Nigel Unwin awarded Gjønnes Medal in Electron…
https://www2.mrc-lmb.cam.ac.uk/richard-henderson-nigel-unwin-awarded-gjonnes-medal-electron-crystallography/29 Aug 2017: The International Union of Crystallography (IUCr) Gjønnes Medal in Electron Crystallography has been awarded to Richard Henderson, Group Leader in the -
LMB postdoc, Tobias Wauer, wins the 2017 EMPRIS Award for Research in …
https://www2.mrc-lmb.cam.ac.uk/lmb-postdoc-tobias-wauer-wins-2017-empris-award-research-brain-diseases/13 Dec 2017: LMB postdoctoral researcher, Tobias Wauer, has won the 2017 EMPRIS Award for Research in Brain Diseases for his work on the molecular causes of -
Student artist meets LMB scientist who discovered inspirational…
https://www2.mrc-lmb.cam.ac.uk/student-artist-meets-lmb-scientist-discovered-inspirational-toxins-structure/9 Mar 2017: Vivian, student artist who created a painting based on lysenin during Protein Data Bank in Europe’s art-science collaborative project, met with LMB’s -
Alison Woollard appointed Academic Champion for Public Engagement…
https://www2.mrc-lmb.cam.ac.uk/alison-woollard-appointed-academic-champion-public-engagement-research-oxford/15 Feb 2017: Alison Woollard, LMB 1996-2000, Postdoctoral Fellow, Cell Biology Professor Alison Woollard, Department of Biochemistry and Fellow of Hertford College, -
Visualising the genome: Cambridge scientists create first 3D…
https://www2.mrc-lmb.cam.ac.uk/visualising-genome-cambridge-scientists-create-first-3d-structures-active-dna/16 Mar 2017: Tim Stevens, in collaboration with scientists from the University of Cambridge, has determined the first 3D structures of intact mammalian genomes from -
Rebecca Taylor to talk at Cambridge Science Festival: Thursday 23rd…
https://www2.mrc-lmb.cam.ac.uk/rebecca-taylor-talk-cambridge-science-festival-thursday-23rd-march-6-00pm/28 Feb 2017: Rebecca Taylor will be giving a talk at the UTC Cambridge as part of the Cambridge Science Festival. In her talk entitled, 'Finding the fountain of youth: -
Hundreds of children and adults enjoy the LMB’s ‘See Your Cells’…
https://www2.mrc-lmb.cam.ac.uk/hundreds-children-adults-enjoy-lmbs-see-cells-activity-cambridge-science-festival/23 Mar 2017: The first weekend of the 2017 Cambridge Science Festival saw over 3000 visitors attend the 'Hands-on at the Guildhall' event, where the LMB’s 'See Your -
Finding the founding fathers of molecular biology – a blog post by…
https://www2.mrc-lmb.cam.ac.uk/finding-founding-fathers-molecular-biology-blog-post-lmb-phd-student-lisa-strittmatter/6 Dec 2017: Lisa Strittmatter, PhD student in the LMB’s Structural Studies Division, explores a recent talk given at the LMB by Professor Sir John Meurig Thomas, and -
G-protein coupled receptors as drug targets: Heptares Therapeutics…
https://www2.mrc-lmb.cam.ac.uk/g-protein-coupled-receptors-drug-targets-heptares-therapeutics-celebrates-tenth-anniversary/6 Jul 2017: Chris Tate – co-founder of Heptares Therapeutics, a company established to commercialise research from the LMB – discusses his work on G-protein coupled -
Scientists establish a comprehensive protein interactions map of the…
https://www2.mrc-lmb.cam.ac.uk/scientists-establish-comprehensive-protein-interactions-map-replication-machinery-chronic-virus/21 Dec 2017: Chronic viral infections are amongst the biggest threats to human health worldwide. Leo James’ group from the LMB’s PNAC Division, in collaboration with -
LMB scientist, Glenn Masson, spends a week shadowing MP for…
https://www2.mrc-lmb.cam.ac.uk/lmb-scientist-glenn-mason-spends-week-shadowing-mp-cambridge-daniel-zeichner-royal-societys-pairing-scheme/13 Dec 2017: Glenn Masson has spent a week shadowing Daniel Zeichner, MP for Cambridge, through the Royal Society’s Pairing Scheme, an initiative which pairs -
Pymol guide
https://www2.mrc-lmb.cam.ac.uk/groups/nagai/download/pymolguide.pdf19 Dec 2017: So if you click on (sele) you can toggle and untoggle your new selection. ... So if you want to make a new selection, make sure you deselect everything first! -
6. Image refinement Sjors Scheres EM course 2017 Agenda ...
https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-06-refinement/?wpdmdl=18760&refresh=66880952d9e9417201913144 Oct 2017: calculate new 2D average as probability weighted averages. Reference-free 2D class averaging. ... The bad news • The experimental data alone is not enough to determine a unique solu7on! • -
Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf29 May 2017: Added into a new N-linked tree: – paucimannose. 23/40. Xyl-β1,2. Xyl - β1,2 – Man – using XYP (beta D xylosepyranose). – ... both GUI interface and built into the auto-builder. New Interface. -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: 3), requiring RsaA to adoptslightly different conformations. Furthermore, gaps in the latticewill be needed for insertion of new RsaA molecules on growingcells. ... The new culture was grown at 30 C for2–3 days. The culture was imaged using a -
Tomography_2017
https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-09-tomography/?wpdmdl=18762&refresh=668809531a5d417201913156 Oct 2017: Missing information due to discrete sampling. Data collection. Set new tilt angle. ... aligned toreference subtomograms. (aligned). averaged subtomograms(new reference). use new reference for alignmentiterate until reference is stable. -
ligand-fitting-tutorial.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ligand-fitting-tutorial.pdf24 Oct 2017: The centre of the screen is a bit crowded now. Undisplay those new ligands. ... When we are happy with our fit, we can merge this new ligand into the mainprotein ligand as we did before. -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: dejongeii DSM 12251 (PdBtubAB), a stop codon (TAA)was introduced before the C-terminal hexahistidine tag in the BtubAB bacterialT7 expression plasmid used (7) by Q5 mutagenesis (New England Biolabs). ... E5954 | -
Fei Long MRC Laboratory of Molecular Biology, Cambridge, U.K. ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Ligand_meeting_Sept_29_2017.pdf29 Sep 2017: new_atom_id _chem_mod_atom.new_type_symbol _chem_mod_atom.new_type_energy _chem_mod_atom.new_charge TMPmod1 delete H2. ... chem_mod_bond.new_type _chem_mod_bond.new_value_dist _chem_mod_bond.new_value_dist_esd TMPmod1 delete C1 H2 single. -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: These constructs are in a vector (provided with the PURE system kit from New England Biolabs) that contains a T7 promoter and transcriptional terminator. ... The resin was washed four times with 1 ml PSB2 and transferred to a new tube after the fourth -
A Polymerization-Associated Structural Switch in FtsZ That Enables…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf3 May 2017: the divisome around the division site (17, 18).These findings have resurrected an old model of bacterial cell division: the templatemodel, in which the closing septum constricts by new cell wall ... because the energetic cost of losing subunits from ends -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: using the Gibson Assembly kit (New England Biolabs). -
REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf19 Jan 2017: PURE system translation reactions. Initial experiments used the PURE translation system from New England Biolabs as per the manufacturer’s instructions. -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf22 Sep 2017: authors. Thesedevelopments along with advances in structure determination techniques (mainly the use ofsynchrotrons, area detectors and crystal freezing) led to an enormous increase in new proteinstructures: in 1990, about 500 structures
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