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  2. Using cryo-EM to solve the structure of a protein complex critical…

    https://www2.mrc-lmb.cam.ac.uk/using-cryo-em-solve-structure-protein-complex-critical-gene-expression/
    Thumbnail for Using cryo-EM to solve the structure of a protein complex critical for gene expression - MRC Laboratory of Molecular Biology 7 Nov 2017: A Log-in or subscription may be required to view this article from the host site: https://www.drugtargetreview.com/news/27088/cryo-electron-microscopy-cpf/].
  3. Network control principles predict neuron function in the C. elegans…

    https://www2.mrc-lmb.cam.ac.uk/network-control-principles-predict-neuron-function-c-elegans-connectome/
    Thumbnail for Network control principles predict neuron function in the C. elegans connectome - MRC Laboratory of Molecular Biology 19 Oct 2017: This work provides new and powerful theoretical tools to allow us to interpret these brain maps and understand the how the nervous system works at the most fundamental, cellular level.
  4. Wojciech Galej receives Early Career Research Award from the…

    https://www2.mrc-lmb.cam.ac.uk/wojciech-galej-receives-early-career-research-award-biochemical-society/
    Thumbnail for Wojciech Galej receives Early Career Research Award from the Biochemical Society - MRC Laboratory of Molecular Biology 11 Apr 2017: Wojciech Galej has been awarded the 2018 Early Career Research Award for Molecular Structure and Function by the Biochemical Society. Wojciech has made
  5. 1 JED: Questions Arising ● Does the Acedrg new ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/JED-questions-arising.pdf
    30 Sep 2017: i.e., use the same description and apply it to the new ligand in question, just like for sugar links. ... generated by Acedrg's new mode). We don't have anything new to offer for modifications.
  6. Richard Henderson and Nigel Unwin awarded Gjønnes Medal in Electron…

    https://www2.mrc-lmb.cam.ac.uk/richard-henderson-nigel-unwin-awarded-gjonnes-medal-electron-crystallography/
    Thumbnail for Richard Henderson and Nigel Unwin awarded Gjønnes Medal in Electron Crystallography - MRC Laboratory of Molecular Biology 29 Aug 2017: The International Union of Crystallography (IUCr) Gjønnes Medal in Electron Crystallography has been awarded to Richard Henderson, Group Leader in the
  7. LMB postdoc, Tobias Wauer, wins the 2017 EMPRIS Award for Research in …

    https://www2.mrc-lmb.cam.ac.uk/lmb-postdoc-tobias-wauer-wins-2017-empris-award-research-brain-diseases/
    Thumbnail for LMB postdoc, Tobias Wauer, wins the 2017 EMPRIS Award for Research in Brain Diseases - MRC Laboratory of Molecular Biology 13 Dec 2017: LMB postdoctoral researcher, Tobias Wauer, has won the 2017 EMPRIS Award for Research in Brain Diseases for his work on the molecular causes of
  8. Student artist meets LMB scientist who discovered inspirational…

    https://www2.mrc-lmb.cam.ac.uk/student-artist-meets-lmb-scientist-discovered-inspirational-toxins-structure/
    Thumbnail for Student artist meets LMB scientist who discovered inspirational toxin's structure - MRC Laboratory of Molecular Biology 9 Mar 2017: Vivian, student artist who created a painting based on lysenin during Protein Data Bank in Europe’s art-science collaborative project, met with LMB’s
  9. Alison Woollard appointed Academic Champion for Public Engagement…

    https://www2.mrc-lmb.cam.ac.uk/alison-woollard-appointed-academic-champion-public-engagement-research-oxford/
    Thumbnail for Alison Woollard appointed Academic Champion for Public Engagement with Research at Oxford - MRC Laboratory of Molecular Biology 15 Feb 2017: Alison Woollard, LMB 1996-2000, Postdoctoral Fellow, Cell Biology  Professor Alison Woollard, Department of Biochemistry and Fellow of Hertford College,
  10. Visualising the genome: Cambridge scientists create first 3D…

    https://www2.mrc-lmb.cam.ac.uk/visualising-genome-cambridge-scientists-create-first-3d-structures-active-dna/
    Thumbnail for Visualising the genome: Cambridge scientists create first 3D structures of active DNA - MRC Laboratory of Molecular Biology 16 Mar 2017: Tim Stevens, in collaboration with scientists from the University of Cambridge, has determined the first 3D structures of intact mammalian genomes from
  11. Rebecca Taylor to talk at Cambridge Science Festival: Thursday 23rd…

    https://www2.mrc-lmb.cam.ac.uk/rebecca-taylor-talk-cambridge-science-festival-thursday-23rd-march-6-00pm/
    Thumbnail for Rebecca Taylor to talk at Cambridge Science Festival: Thursday 23rd March, 6.00pm - MRC Laboratory of Molecular Biology 28 Feb 2017: Rebecca Taylor will be giving a talk at the UTC Cambridge as part of the Cambridge Science Festival. In her talk entitled, 'Finding the fountain of youth:
  12. Hundreds of children and adults enjoy the LMB’s ‘See Your Cells’…

    https://www2.mrc-lmb.cam.ac.uk/hundreds-children-adults-enjoy-lmbs-see-cells-activity-cambridge-science-festival/
    Thumbnail for Hundreds of children and adults enjoy the LMB’s ‘See Your Cells’ activity at the Cambridge Science Festival - MRC Laboratory of Molecular Biology 23 Mar 2017: The first weekend of the 2017 Cambridge Science Festival saw over 3000 visitors attend the 'Hands-on at the Guildhall' event, where the LMB’s 'See Your
  13. Finding the founding fathers of molecular biology – a blog post by…

    https://www2.mrc-lmb.cam.ac.uk/finding-founding-fathers-molecular-biology-blog-post-lmb-phd-student-lisa-strittmatter/
    Thumbnail for Finding the founding fathers of molecular biology – a blog post by LMB PhD student, Lisa Strittmatter - MRC Laboratory of Molecular Biology 6 Dec 2017: Lisa Strittmatter, PhD student in the LMB’s Structural Studies Division, explores a recent talk given at the LMB by Professor Sir John Meurig Thomas, and
  14. G-protein coupled receptors as drug targets: Heptares Therapeutics…

    https://www2.mrc-lmb.cam.ac.uk/g-protein-coupled-receptors-drug-targets-heptares-therapeutics-celebrates-tenth-anniversary/
    Thumbnail for G-protein coupled receptors as drug targets: Heptares Therapeutics celebrates its tenth anniversary - MRC Laboratory of Molecular Biology 6 Jul 2017: Chris Tate – co-founder of Heptares Therapeutics, a company established to commercialise research from the LMB – discusses his work on G-protein coupled
  15. Scientists establish a comprehensive protein interactions map of the…

    https://www2.mrc-lmb.cam.ac.uk/scientists-establish-comprehensive-protein-interactions-map-replication-machinery-chronic-virus/
    Thumbnail for Scientists establish a comprehensive protein interactions map of the replication machinery of a chronic virus - MRC Laboratory of Molecular Biology 21 Dec 2017: Chronic viral infections are amongst the biggest threats to human health worldwide. Leo James’ group from the LMB’s PNAC Division, in collaboration with
  16. LMB scientist, Glenn Masson, spends a week shadowing MP for…

    https://www2.mrc-lmb.cam.ac.uk/lmb-scientist-glenn-mason-spends-week-shadowing-mp-cambridge-daniel-zeichner-royal-societys-pairing-scheme/
    Thumbnail for LMB scientist, Glenn Masson, spends a week shadowing MP for Cambridge, Daniel Zeichner, through the Royal Society’s Pairing Scheme - MRC Laboratory of Molecular Biology 13 Dec 2017: Glenn Masson has spent a week shadowing Daniel Zeichner, MP for Cambridge, through the Royal Society’s Pairing Scheme, an initiative which pairs
  17. Pymol guide

    https://www2.mrc-lmb.cam.ac.uk/groups/nagai/download/pymolguide.pdf
    19 Dec 2017: So if you click on (sele) you can toggle and untoggle your new selection. ... So if you want to make a new selection, make sure you deselect everything first!
  18. 6. Image refinement Sjors Scheres EM course 2017 Agenda ...

    https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-06-refinement/?wpdmdl=18760&refresh=66880952d9e941720191314
    4 Oct 2017: calculate new 2D average as probability weighted averages. Reference-free 2D class averaging. ... The bad news • The experimental data alone is not enough to determine a unique solu7on! •
  19. Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf
    29 May 2017: Added into a new N-linked tree: – paucimannose. 23/40. Xyl-β1,2. Xyl - β1,2 – Man – using XYP (beta D xylosepyranose). – ... both GUI interface and built into the auto-builder. New Interface.
  20. Structure of the hexagonal surface layer on Caulobacter crescentus…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf
    6 Apr 2017: 3), requiring RsaA to adoptslightly different conformations. Furthermore, gaps in the latticewill be needed for insertion of new RsaA molecules on growingcells. ... The new culture was grown at 30 C for2–3 days. The culture was imaged using a
  21. Tomography_2017

    https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-09-tomography/?wpdmdl=18762&refresh=668809531a5d41720191315
    6 Oct 2017: Missing information due to discrete sampling. Data collection. Set new tilt angle. ... aligned toreference subtomograms. (aligned). averaged subtomograms(new reference). use new reference for alignmentiterate until reference is stable.
  22. ligand-fitting-tutorial.dvi

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ligand-fitting-tutorial.pdf
    24 Oct 2017: The centre of the screen is a bit crowded now. Undisplay those new ligands. ... When we are happy with our fit, we can merge this new ligand into the mainprotein ligand as we did before.
  23. Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf
    29 Dec 2017: dejongeii DSM 12251 (PdBtubAB), a stop codon (TAA)was introduced before the C-terminal hexahistidine tag in the BtubAB bacterialT7 expression plasmid used (7) by Q5 mutagenesis (New England Biolabs). ... E5954 |
  24. Fei Long MRC Laboratory of Molecular Biology, Cambridge, U.K. ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Ligand_meeting_Sept_29_2017.pdf
    29 Sep 2017: new_atom_id _chem_mod_atom.new_type_symbol _chem_mod_atom.new_type_energy _chem_mod_atom.new_charge TMPmod1 delete H2. ... chem_mod_bond.new_type _chem_mod_bond.new_value_dist _chem_mod_bond.new_value_dist_esd TMPmod1 delete C1 H2 single.
  25. REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf
    4 Aug 2017: These constructs are in a vector (provided with the PURE system kit from New England Biolabs) that contains a T7 promoter and transcriptional terminator. ... The resin was washed four times with 1 ml PSB2 and transferred to a new tube after the fourth
  26. A Polymerization-Associated Structural Switch in FtsZ That Enables…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf
    3 May 2017: the divisome around the division site (17, 18).These findings have resurrected an old model of bacterial cell division: the templatemodel, in which the closing septum constricts by new cell wall ... because the energetic cost of losing subunits from ends
  27. IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf
    13 Jan 2017: using the Gibson Assembly kit (New England Biolabs).
  28. REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf
    19 Jan 2017: PURE system translation reactions. Initial experiments used the PURE translation system from New England Biolabs as per the manufacturer’s instructions.
  29. doi:10.1016/j.pbiomolbio.2004.07.009

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf
    22 Sep 2017: authors. Thesedevelopments along with advances in structure determination techniques (mainly the use ofsynchrotrons, area detectors and crystal freezing) led to an enormous increase in new proteinstructures: in 1990, about 500 structures

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