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1 JED: Questions Arising ● Does the Acedrg new ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/JED-questions-arising.pdf30 Sep 2017: i.e., use the same description and apply it to the new ligand in question, just like for sugar links. ... generated by Acedrg's new mode). We don't have anything new to offer for modifications. -
Pymol guide
https://www2.mrc-lmb.cam.ac.uk/groups/nagai/download/pymolguide.pdf19 Dec 2017: So if you click on (sele) you can toggle and untoggle your new selection. ... So if you want to make a new selection, make sure you deselect everything first! -
6. Image refinement Sjors Scheres EM course 2017 Agenda ...
https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-06-refinement/?wpdmdl=18760&refresh=667ebebe4c11e17195823984 Oct 2017: calculate new 2D average as probability weighted averages. Reference-free 2D class averaging. ... The bad news • The experimental data alone is not enough to determine a unique solu7on! • -
Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf29 May 2017: Added into a new N-linked tree: – paucimannose. 23/40. Xyl-β1,2. Xyl - β1,2 – Man – using XYP (beta D xylosepyranose). – ... both GUI interface and built into the auto-builder. New Interface. -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: 3), requiring RsaA to adoptslightly different conformations. Furthermore, gaps in the latticewill be needed for insertion of new RsaA molecules on growingcells. ... The new culture was grown at 30 C for2–3 days. The culture was imaged using a -
Tomography_2017
https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-09-tomography/?wpdmdl=18762&refresh=667ebebe76a5e17195823986 Oct 2017: Missing information due to discrete sampling. Data collection. Set new tilt angle. ... aligned toreference subtomograms. (aligned). averaged subtomograms(new reference). use new reference for alignmentiterate until reference is stable. -
ligand-fitting-tutorial.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ligand-fitting-tutorial.pdf24 Oct 2017: The centre of the screen is a bit crowded now. Undisplay those new ligands. ... When we are happy with our fit, we can merge this new ligand into the mainprotein ligand as we did before. -
Fei Long MRC Laboratory of Molecular Biology, Cambridge, U.K. ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Ligand_meeting_Sept_29_2017.pdf29 Sep 2017: new_atom_id _chem_mod_atom.new_type_symbol _chem_mod_atom.new_type_energy _chem_mod_atom.new_charge TMPmod1 delete H2. ... chem_mod_bond.new_type _chem_mod_bond.new_value_dist _chem_mod_bond.new_value_dist_esd TMPmod1 delete C1 H2 single. -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: dejongeii DSM 12251 (PdBtubAB), a stop codon (TAA)was introduced before the C-terminal hexahistidine tag in the BtubAB bacterialT7 expression plasmid used (7) by Q5 mutagenesis (New England Biolabs). ... E5954 | -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: These constructs are in a vector (provided with the PURE system kit from New England Biolabs) that contains a T7 promoter and transcriptional terminator. ... The resin was washed four times with 1 ml PSB2 and transferred to a new tube after the fourth
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