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12 Nov 2009: LMB). The concept integrates several innovative approaches,such as chemically compatible mixes of potential ligands, new buffer systems andprecipitant mixes that also act as cryoprotectants. ... Both assumptions were a driving force behind my attemptsto

21 Mar 2003: We interpret theseresults to indicate that cyclosporin A is inhibiting theendocytic formation of new synaptic vesicles that normallyoccurs when the synaptosomes are depolarised. ... a single site that defines a new SH3 bindingconsensus sequence.

20 Apr 2002: Brünger, A. (1992) X-PLOR Manual (Yale Univ., New Haven,. CT).26. Laskowski, R.

1 Mar 2024: All enzymes for DNA amplification (Phusion Polymerase) and plasmid construction (restriction enzymes, T4 DNA ligase and Quick CIP) were obtained from New England Biolabs (NEB) and used according to the

8 Jun 2020: J. Begg, S. J. Dewar, W. D. Donachie, A new Escherichia coli cell division gene, ftsK. ... Nucleic Acids Res. 32, 5260–5279 (2004). 28. J. Zivanov et al., New tools for automated high-resolution cryo-EM structure de-termination in RELION-3.

8 Jul 1998: 1997) J. Biol. Chem. 272, 11629–1163524 Folli, F. et al. (1993) New Engl.

18 Jan 2018: as well as entirely new classes of filaments16–18, leading to an expanded concept of what a cytoskeleton is (BOX 1). ... In many rod‑shaped bacteria, dynamic MreB filaments control the synthesis of a new cell wall to maintain shape.

20 Feb 2001: L. (1998).Crystallography and NMR system: a new softwaresuite for macromolecular structure determination.Acta Crystallog.

16 Jul 2023: This makes itpossible to visualize new DNA synthesis through the incorporationof the thymidine analog EdU by fluorescence microscopy (29). ... Fragmentswere cloned into the pSVAaraFX backbone for Vps4E209Q and thepSVAaraHA-stop backbone for CdvBΔMIM2,

20 May 2010: Corresponding authors. LW Hamoen, The Centre for Bacterial CellBiology, Medical Science New Building, Medical School, NewcastleUniversity, Richardson Road, Newcastle upon Tyne NE2 4AX, UK.Tel.: þ44 191 208 3240; Fax: þ44 ... These were then removed

11 Jun 2013: Molecular replacement. New structures from old. The Phase Problem. amplitude. phase.

1 May 2004: The SulA fold is related to a fragment of RecA, but this seems accidental andprobably only shows how nature reuses successful designs for new purposes. ... For GFP labeling, new genetic tools in mollicutes must be devel-oped for that purpose.

26 Aug 2019: from genomic DNA and cloned into vector pET15b using Gibson assembly (New England Biolabs). ... plasmid pHis17 using Gibson assembly (New England Biolabs) with a stop codon before the C-terminal tag on the plasmid.

17 Sep 2008: The adaptor protein ParR has ‘formin-like activity’, meaning. t actively participates in the addition of new ParM subunits to thend.

30 Jun 2004: Molecular Microbiology (2004). 53. (2), 419–432 doi:10.1111/j.1365-2958.2004.04133.x. 2004 Blackwell Publishing Ltd. Blackwell Science, LtdOxford, UKMMIMolecular Microbiology0950-382XBlackwell Publishing Ltd, 2004? 2004. 53. 2419432. Original

6 May 1999: digested with SI and NotI(New England Biolabs), and ligated into the SI and NotIdigested pUC119His6 (Holliger & Riechmann, 1997). ... Filamentous bacterio-phages. In The Bacteriophages (Calendar, R., ed.), pp.375-456, Plenum Press, New York.

11 Nov 2019: Faster refinement goes hand in hand with the addition of new restraint types. ... library. The new mode of Acedrg allows the generation of bespoke chemical links,.

7 Dec 2012: The title. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. ... new,higher steady state.

29 Aug 2021: High-resolution structure deter-mination may aid in these directions as new compounds are developed andtested as treatments for COVID-19. ... 9, 2. 021. 20. J. Zivanov et al., New tools for automated high-resolution cryo-EM structuredetermination in

10 Dec 2014: INVITED REVIEW. Mechanosensory molecules and circuits in C. elegans. William R. Schafer. Received: 3 June 2014 /Revised: 30 June 2014 /Accepted: 2 July 2014 /Published online: 23 July 2014# The Author(s) 2014. This article is published with open

27 Aug 2015: Coot displays a Map Colour Selection window]. • Choose a new colour by clicking on the colour widgets. ... We want a CYS:. • Mutate & Auto Fit. • Click on an atom in the new ALA.

16 Jul 2015: wwPDB Ligands. Year on year increase in the number of new entities. ... 6943 new ligand types (88%) are non-polymers. – LINKed ligands excluded.

27 Nov 2003: can be deliv-ered to the new cell wall and/or to remodel the newlyfused membrane. ... a mechanism wouldhelp ensure an even insertion of new lipid along thelength of the cell.

21 Nov 2008: redox perturbations), thereby providing a new tool for exploring the ER. ... This new molecular tool can now be combined with the vast collections of yeast strains (deletion, hypomorphic, overexpression, and other mutations) to reveal previously

28 Nov 2006: S. & Vallee, R. B.Molecular cloning of the microtubule-associated mechanochemical enzymedynamin reveals homology with a new family of GTP-binding proteins.

19 Jun 2006: Intriguing new studiesindicate a potentially key role for MVB pathways in prion transmis-sion and metabolism, reinforcing the central importance of proteintransport pathways as the molecular basis of various diseases. ... Validation of thisprovocative

31 Oct 2007: 1) The tethered, ADP-bound lead head is free to find a new binding site. ... N., Muller,J. and Mandelkow, E. (2000). A new look at the microtubule binding patterns ofdimeric kinesins.

14 Jan 2002: Lloyd, C.W. Microtubules (Wiley-Liss, New York, 1993). 2. 3m. 4C. ... Hol, W.G. Crystal structure of glycosomal glyceraldehyde-3-. phosphate dehydrogenase from Leishmania mexicana: implications for structure-based drug design and new position for the

11 Apr 2024: signalling. EMBO J 18, 1630-1641. 60. Yu X, Waltzer L & Bienz M (1999) A new Drosophila APC homologue associated with. ... Science 316, 1619-1622. 93. Tran H, Hamada F, Schwarz-Romond T & Bienz M (2008) Trabid, a new positive regulator of.

28 Apr 2010: There are still questions about how the structure recognizes the very slight curvature of the polar membrane, but the new structural information greatly advances the discussion.

26 Jun 2015: Discard the pipet tip, add a new pipet tip and pipet 1 ml of Crystal Screen Lite reagent 2 into reservoir A2.

14 May 2013: structure determination and explains why the development of more. efficient crystallization screens is still challenging (McPherson &. Cudney, 2006). We are constantly testing new conditions and will. eventually integrate them into our

28 Jul 2020: L. D. & Kirshner, D. A. (1971). Nature New Biol. 231,.

6 Mar 2001: single site that denes a new SH3 binding consensus sequence. J. ... Medicine, Oxford University, Roosevelt Drive, Oxford OX3 7BN, UK. The Oxford Centre for Molecular Sciences, New Chemistry, South Parks Road,Oxford OX1 3QT, UK These authors contributed

29 Jan 2024: 2008. 4: 232-234. [Featured in Nature 2008. 45: 868, Chemistry & Engineering News (ACS). ... 2006. 2: 254-258. [News & Views, Nature Chem. Biol. 2006. 2: 231-232].

8 Jun 2006: oxyl-2,2,5,5-tetramethylpyrroline-3-methyl]-methanethiosulfonate) to generate the new side. chain R1 using a previously described protocol (Jao et al., 2004).

6 Dec 2014: analyses, we show that MinD activates MinC by forming a new class of alternating. ... We combined our new crystalstructure with the EcMinD dimer structure, revealing twointeresting features (Fig.

23 Dec 2013: The G109K substitution has a dramatic ef-fect, because in the new crystals the protein filaments are absent,but dimers are still present (Fig. ... Construction of Plasmids for Protein Purification. pMal-C2X (New EnglandBiolabs) was used to clone and

3 Dec 2004: 17,4943 (1998). 35. A. G. W. Leslie, in Joint CCP4 and ESF-EACMB News-letter on Protein Crystallography No.

30 Jun 2003: Clearly, establishingany relationship between PrPSc and cyPrP represents aformidable challenge and will necessitate the developmentof new tools to readily detect and modulate cyPrPformation in precise and selective ways.

3 Dec 2004: 17,4943 (1998). 35. A. G. W. Leslie, in Joint CCP4 and ESF-EACMB News-letter on Protein Crystallography No.

18 Dec 2008: The title. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005.

8 Sep 2006: The new crystal structures are thought to resemblemyosin bound to an actin filament in the rigor state. ... domains binding to new sites on the MT. turn can affect the conformation of switch I and the nu-cleotide binding pocket near switch I.

25 Jul 2006: m (22). Hence, each spinehead is not only optimallyseparated from its neighbors in terms of efficient ‘‘scanning’’ ofthe surrounding space but also is positioned appropriately tomake synaptic contact with a new

20 Apr 1999: are more closely related to dinucleotide-bindingproteins (Rossmann fold proteins) than to classicalGTPases but should be treated as a new family of GTPases(Nogaleset al., 1998a).

3 May 2002: J. Biol. Chem. 275, 38597–38604. 33 Darrouzet, E. et al. (2001) Large scale domainmovement in cytochrome bc1: a new device forelectron transfer in proteins.

12 Oct 2006: culture in a New Brunswick Bioflo Pro fermentor and induced with 0.2% (w/v).

4 Oct 2017: calculate new 2D average as probability weighted averages. Reference-free 2D class averaging. ... The bad news • The experimental data alone is not enough to determine a unique solu7on! •

3 Nov 2001: spond to the appearance of new small helices (H10,H20, H200, H30, H90 and H110). ... W., eds), vol. 1, pp. 3 and 32, JohnWiley & Sons, Inc., New York.

4 Jan 2016: A simple filament nomenclature. Since ever more filament systems with new architectures are being. ... CM (2014) Subpolar addition of new cell wall is directed by DivIVA in.