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24 Sep 2015: treatment leads to improvement of ABCG2 mutant trafficking.We first determined whether the increased ABCG2 expressionon the cell surface required new protein synthesis. ... Here, we highlighted a new molecularmechanism, HDIs acting as modulators of

13 Jun 2009: Recent advances in our understanding of normalPrP biosynthesis and degradation might have unexpect-edly shed new light on this complex problem. ... Cell 93, 337–3484 Wickner, R.B. et al. (2004) Prion genetics: new rules for a new kind of.

16 Nov 2005: It is not clear how soon this exchangeable GTP (on the so-called E-site) is hydrolysed after the addition of a new dimer to the plus end but it may

27 Feb 2022: 1200 New York Avenue NW, Washington, DC20005. The title Science is a registered trademark of AAAS.Copyright 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of

10 Nov 2000: Cell-free transcription wasperformed by using SP6 RNA polymerase (New England Bio-labs) as described (23).

19 Dec 2009: body, so slight movements indicated by the new density. have not been adjusted.

24 Sep 2004: Section 1734 solely toindicate this fact. The atomic coordinates and structure factors (code 1uzx) have beendeposited in the Protein Data Bank, Research Collaboratory for Struc-tural Bioinformatics, Rutgers University, New Brunswick,

7 Jul 1999: In Current Protocols in Molecular Biology, vol.Suppl. 6, pp. 8.2.8-8.2.13, John Wiley & Sons, Inc.,New York. ... J. Biol. Chem. 271,663-674. Nicholls, A. (1992). GRASP: Graphic Representation andAnalysis of Surface Properties, Columbia University,New York

23 Oct 2012: The. symmetry of this new volume was determined by a least-squares fitting algorithm, and this symmetry was imposed. ... upon the reconstruction (35). The new symmetry-enforced volume was used as a reference for the next round of.

1 Dec 2000: and the PI3Kg RBD, strand(Figure 2A), presumably by forming a new salt-link be-b2 of Ras is aligned with PI3Kg strand Rb2 (Figure 3B).tween the catalytic domain and ... H-Ras was loaded with GMPPNP using the alkaline phos-in conjunction with the novel

2 Aug 2010: This conformational changelifts the phenylalanine residue of the DFG motif to disruptits interaction with helix αC, thereby creating a new hydro-phobic pocket deep behind the ATP-binding site that has ... For these inhibitors, a new pocket is formed

13 Jun 2022: Nature Communications, doi:10.1038/s41467-022-30656-2

20 Apr 2002: Each new water molecule was then mapped ontothe native structure by applying the transformation operatorof the domain to which it was the closest neighbor. ... This new structural information is incorporatedinto a detailed reaction mechanism for PLC-δ1

21 Feb 2011: This zippering explains how generation of aSNARE complex brings the membranes together, but doesnot explain the mechanism of membrane fusion per se [53].New results show that SNARE assembly tends to

4 Aug 2017: These constructs are in a vector (provided with the PURE system kit from New England Biolabs) that contains a T7 promoter and transcriptional terminator. ... The resin was washed four times with 1 ml PSB2 and transferred to a new tube after the fourth

6 Mar 2008: Here we use. xvi. automated methods to automatically detect these activities, which rely in part on a new method. ... These new. methods have made it possible to reliably detect events that are time-consuming and laborious to.

25 Sep 2008: Therefore, genetic mutations thatimpart a new or aberrant property on the protein to causecellular dysfunction are termed gain-of-function (GOF)diseases.

30 May 1998: However, a new classof topogenic sequences called “pause-transfer sequences”has been identified in apoB (9–11). ... Blackhart, B. J. McCarthy, and V. R. Lin-gappa. 1990. New variation on the translocation of proteins duringearly biogenesis of

6 Apr 2005: Nevertheless, thechannels in the new 3D maps are of better qualitythan those in previous maps, due to the extensive. ... Discussion. The present work provides 3D maps of the RCCthat show new features of the junction and theprotein translocation channel.

29 May 2019: the presence of 100 mg/ml cycloheximide to suppress new protein synthesis. ... tion attenuation during acute ER stress, new GFP-PrP synthesis during the time course is relatively.

15 Mar 2018: As the. motions of the Rod complexes reflect the insertion of new cell wall, their circumferential motions. ... along a perfectly ordered template. As MreB movement reflects the insertion of new glycan strands,.

24 Jan 2005: doi:10.1016/j.jmb.2004.12.031 J. Mol. Biol. (xxxx) xx, 1–23. ARTICLE IN PRESS. Refined Structure of the Nicotinic AcetylcholineReceptor at 4 Å Resolution. Nigel Unwin. MRC Laboratory of MolecularBiology, Hills Road, CambridgeCB2 2QH, UK.

22 Jul 2019: form a new class of alternating, copolymeric filaments,. which can be assembled in vitro either on lipid mem-. ... Hagen WJ, Lindahl E and Scheres SH (2018) New. tools for automated high-resolution cryo-EM structure.

14 Jun 2006: terest as a new class of drug targets (Ward et al., 2003).

30 Jul 2013: Nicotinic acetylcholinereceptorandthestructuralbasisofneuromuscular transmission:insightsfromTorpedopostsynapticmembranes. Nigel UnwinMRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK. Abstract. The nicotinic

17 Jun 2005: 1997). The SH3 domain of amphiphysinbinds the proline-rich domain of dynamin at a single site that de-fines a new SH3 binding consensus sequence.

8 Mar 2019: 3). First, the load of new proteinsentering stressed mitochondria is reduced at theexpense of generating mislocalized proteins thatmust be degraded by cytosolic proteasomes(Wright et al.

2 Jun 2007: Structure. Article. Please cite this article in press as: Henne et al., Structure and Analysis of FCHo2 F-BAR Domain: A Dimerizing and Membrane RecruitmentModule that Effects Membrane Curvature, Structure (2007), doi:10.1016/j.str.2007.05.002.

7 Apr 2010: Processes of membrane shaping belonging to the second classinclude transient distortions of the membrane continuity and re-connections of the membrane surface in a new way.

6 Jun 2014: The elongasome ensures that new glycan strands cross-linked by short peptides are inserted along the peptidoglycan scaffold during cell elongation (den Blaauwen et al., 2008; Goffin and Ghuysen, 1998; Matsuhashi

20 Apr 2002: Struct. Biol. 8, 526–530.Pannu, N.S., et al. (1998). Crystallography & NMR system: a new.

16 Oct 2003: This increase in OSER fluorescence intensity was notsolely due to new protein synthesis. ... The primers convert leucine 221 tolysine and introduce a new restriction site, Afl2, with a silent mutation forease of screening mutants.

23 Nov 2004: Indi- 11. Corvera, S. (2001). Phosphatidylinositol 3-kinase and the controlrect immunofluorescence was performed on cells permeabilized of endosome dynamics: New players defined by structural mo-with 0.1% Triton

25 Apr 1997: Finally,. we show that during a translocational pause, coordinateis translocationally paused, the proteins adjacent to ithave changed: a new protein of 11 kDa moves into a changes occur in the protein

19 Aug 2015: The eRF1(AAQ) mutant was generated via Phusion mutagenesis.Antibodies against Hbs1 and ABCE1 have been described32; the eRF1 antibodywas from New England Biolabs.Release factor purification.

3 May 2017: the divisome around the division site (17, 18).These findings have resurrected an old model of bacterial cell division: the templatemodel, in which the closing septum constricts by new cell wall ... because the energetic cost of losing subunits from ends

27 Mar 2012: protofilament architecture (Figures 2A and 3). Inspection of our new FtsA/FtsZ crystal structure. ... in a Beckman TLA100rotor for 30 min, supernatants transferred to new tubes and GTP(in case of FtsZ) or ATP (in case of ParM-Z hybrid) to a

15 Nov 2011: Better understanding of the mechanism and cellmachinery responsible for driving fusion pore expansion in cell-to-cell fusion will bring about new ways of controlling fusion indevelopment and in pathological conditions.

30 Apr 2010: Stable for at least 2 years.4. T7 or SP6 polymerase (New England Biolabs). ... chromatography resin, wash extensively with bufferscontaining the desired detergent, and elute in a buffer contain-ing this new detergent.

23 Nov 2004: C., Blondeau, F., and McPherson, P. S.(2003). Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif. ... P.,and De Camilli, P. (1999). The epsins define a family of proteins that interact withcomponents of the

13 Jan 2017: using the Gibson Assembly kit (New England Biolabs).

22 Mar 2005: Developmental Cell, Vol. 6, 525–538, April, 2004, Copyright 2004 by Cell Press. Clathrin Adaptor epsinR Is Requiredfor Retrograde Sorting on Early Endosomal Membranes. routes is commonly termed clathrin-independent endo-cytosis (Nichols and

21 Nov 2012: The p110 delta structure: mechanisms for selectivity andpotency of new PI(3)K inhibitors.

12 Nov 2006: Results from these studies provide new insight into how the PI3K-C2α PX domain specifically recognizes PtdIns(4,5)P2 and how the PX and C2. ... Restriction endonucleases and other enzymes for molecular biology were from New England Biolabs (Beverly, MA).

2 Feb 2004: Membrane transport between compartments in eukary-otic cells requires proteins that allow the budding andSCISSION of nascent cargo vesicles from one compartmentand their targeting and fusion with another. Just asSNARES are proposed to be essential

1 Dec 2019: TA proteins in early embryonic development.11–16 This study offers the first evidence for its role in human disease, and provides new insight into the molecular mechanisms in DCM.

18 Oct 2008: essential for induction of synaptic long-term depression. (LTD) (9,10) as well as for a new form of cerebellar synaptic. ... we present data providing new insight into the molecular. mechanisms controlling the activity of the PICK1 BAR.

19 Mar 2013: C.,and De Camilli, P. (1997) The SH3 domain of amphiphysin binds theproline-rich domain of dynamin at a single site that defines a new SH3binding consensus sequence.

5 Sep 2003: Croci, C., Brandstatter, J.H., and Enz, R. (2003). ZIP3, a new spliceisopropanol, and 0.1 M CAPSO (pH 9.0).

26 Jun 2001: B. Prusiner. Restriction enzymes, other DNA-modifyingenzymes, and SP6 RNA polymerase were from New England BiolabsInc.