Search

11 Sep 2003: New channel proteins are being discovered and characterized, Superfamilies of voltage- and ligand-gated channels are being identified and ex- tended. ... without the energetically unfavored pro- cesses of creating substantial new surfaces or invoking

20 Mar 2014: 7. Use a ‘for loop’ to iteratively recreate a new random Bin(100, 0.4) sample of100 observations and overplot the corresponding empirical density functionsonto the plot of the Normal density

4 Aug 2008: ParM filaments while allowing entry of new ATP-bound. monomers. We propose a processive mechanism by which. ... The binding of a new subunit. induces ATP hydrolysis in the previous subunit of the proto-.

8 Dec 2007: The title. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005.

6 Apr 2017: 3), requiring RsaA to adoptslightly different conformations. Furthermore, gaps in the latticewill be needed for insertion of new RsaA molecules on growingcells. ... The new culture was grown at 30 C for2–3 days. The culture was imaged using a

2 Aug 2010: We devised a new expression and purification strategy by introducing a TEV protease. ... All propeller-shaped inhibitors create a new specificity pocket not present in the apo form of the enzyme.

22 Aug 2007: be appropriately interpreted. Part 3: Clustered-hubs. Clustered-hubs are a new subtype of hubs not previously described. ... new subtype of hubs that we find in CME. These can be composed of proteins with.

28 Apr 2024: contributed new reagents/analytic tools; Y.L. and J.L. analyzed data; and Y.L.

3 Dec 2005: to find out new search regions to detect all the eggs attached to the worm body. ... previously used for the egg detection test and 20 new normal wild type videos.

6 Oct 2017: Missing information due to discrete sampling. Data collection. Set new tilt angle. ... aligned toreference subtomograms. (aligned). averaged subtomograms(new reference). use new reference for alignmentiterate until reference is stable.

23 Dec 2013: CONTENT ALERTS. more>>article), Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this.

1 Mar 2024: All enzymes for DNA amplification (Phusion Polymerase) and plasmid construction (restriction enzymes, T4 DNA ligase and Quick CIP) were obtained from New England Biolabs (NEB) and used according to the

19 Apr 2018: protein droplets (each row now requires 8 new microsyringesto avoid cross-contamination).

14 Sep 2010: Filaments stabilized in this way continue to polymerize with the addition of new ParM subunits at both ParR–parC-capped ends, although the actual rate of elongation of an individual

7 Mar 2019: New insight into a possible functional link be-tween GCN2 and ribosomes came from a recent analysis of micelacking both a specific neuronal tRNA (tRNAArgUCU) and theputative ribosome recycling factor GTPBP2

21 Mar 2003: We interpret theseresults to indicate that cyclosporin A is inhibiting theendocytic formation of new synaptic vesicles that normallyoccurs when the synaptosomes are depolarised. ... a single site that defines a new SH3 bindingconsensus sequence.

12 Nov 2009: LMB). The concept integrates several innovative approaches,such as chemically compatible mixes of potential ligands, new buffer systems andprecipitant mixes that also act as cryoprotectants. ... Both assumptions were a driving force behind my attemptsto

11 Jun 2013: Molecular replacement. New structures from old. The Phase Problem. amplitude. phase.

8 Jun 2020: J. Begg, S. J. Dewar, W. D. Donachie, A new Escherichia coli cell division gene, ftsK. ... Nucleic Acids Res. 32, 5260–5279 (2004). 28. J. Zivanov et al., New tools for automated high-resolution cryo-EM structure de-termination in RELION-3.

18 Jan 2018: as well as entirely new classes of filaments16–18, leading to an expanded concept of what a cytoskeleton is (BOX 1). ... In many rod‑shaped bacteria, dynamic MreB filaments control the synthesis of a new cell wall to maintain shape.

16 Jul 2023: This makes itpossible to visualize new DNA synthesis through the incorporationof the thymidine analog EdU by fluorescence microscopy (29). ... Fragmentswere cloned into the pSVAaraFX backbone for Vps4E209Q and thepSVAaraHA-stop backbone for CdvBΔMIM2,

20 Apr 2002: Brünger, A. (1992) X-PLOR Manual (Yale Univ., New Haven,. CT).26. Laskowski, R.

26 Aug 2019: from genomic DNA and cloned into vector pET15b using Gibson assembly (New England Biolabs). ... plasmid pHis17 using Gibson assembly (New England Biolabs) with a stop codon before the C-terminal tag on the plasmid.

20 May 2010: Corresponding authors. LW Hamoen, The Centre for Bacterial CellBiology, Medical Science New Building, Medical School, NewcastleUniversity, Richardson Road, Newcastle upon Tyne NE2 4AX, UK.Tel.: þ44 191 208 3240; Fax: þ44 ... These were then removed

1 May 2004: The SulA fold is related to a fragment of RecA, but this seems accidental andprobably only shows how nature reuses successful designs for new purposes. ... For GFP labeling, new genetic tools in mollicutes must be devel-oped for that purpose.

8 Jul 1998: 1997) J. Biol. Chem. 272, 11629–1163524 Folli, F. et al. (1993) New Engl.

17 Sep 2008: The adaptor protein ParR has ‘formin-like activity’, meaning. t actively participates in the addition of new ParM subunits to thend.

20 Feb 2001: L. (1998).Crystallography and NMR system: a new softwaresuite for macromolecular structure determination.Acta Crystallog.

11 Nov 2019: Faster refinement goes hand in hand with the addition of new restraint types. ... library. The new mode of Acedrg allows the generation of bespoke chemical links,.

30 Jun 2004: Molecular Microbiology (2004). 53. (2), 419–432 doi:10.1111/j.1365-2958.2004.04133.x. 2004 Blackwell Publishing Ltd. Blackwell Science, LtdOxford, UKMMIMolecular Microbiology0950-382XBlackwell Publishing Ltd, 2004? 2004. 53. 2419432. Original

6 May 1999: digested with SI and NotI(New England Biolabs), and ligated into the SI and NotIdigested pUC119His6 (Holliger & Riechmann, 1997). ... Filamentous bacterio-phages. In The Bacteriophages (Calendar, R., ed.), pp.375-456, Plenum Press, New York.

29 Aug 2021: High-resolution structure deter-mination may aid in these directions as new compounds are developed andtested as treatments for COVID-19. ... 9, 2. 021. 20. J. Zivanov et al., New tools for automated high-resolution cryo-EM structuredetermination in

7 Dec 2012: The title. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. ... new,higher steady state.

11 Jun 2013: Molecular replacement. New structures from old. The Phase Problem. amplitude. phase.

27 Aug 2015: Coot displays a Map Colour Selection window]. • Choose a new colour by clicking on the colour widgets. ... We want a CYS:. • Mutate & Auto Fit. • Click on an atom in the new ALA.

6 Apr 2007: The title SCIENCE is a. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005.

19 Jun 2018: Yeast Mitochondrial Large Ribosomal Subunit. 19/06/18. New Ribosome Components. blue: conservedred: found in bothyellow: yeast only. ... Lots to see at 4 rmsd. Alpha Helix Placement. Scenario: Looking at a new map, not built with automatic tools: “I

3 Dec 2004: 17,4943 (1998). 35. A. G. W. Leslie, in Joint CCP4 and ESF-EACMB News-letter on Protein Crystallography No.

18 Dec 2008: The title. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005.

25 Jul 2006: m (22). Hence, each spinehead is not only optimallyseparated from its neighbors in terms of efficient ‘‘scanning’’ ofthe surrounding space but also is positioned appropriately tomake synaptic contact with a new

3 Nov 2001: spond to the appearance of new small helices (H10,H20, H200, H30, H90 and H110). ... W., eds), vol. 1, pp. 3 and 32, JohnWiley & Sons, Inc., New York.

3 May 2002: J. Biol. Chem. 275, 38597–38604. 33 Darrouzet, E. et al. (2001) Large scale domainmovement in cytochrome bc1: a new device forelectron transfer in proteins.

12 Oct 2006: culture in a New Brunswick Bioflo Pro fermentor and induced with 0.2% (w/v).

20 Apr 1999: are more closely related to dinucleotide-bindingproteins (Rossmann fold proteins) than to classicalGTPases but should be treated as a new family of GTPases(Nogaleset al., 1998a).

4 Jan 2016: A simple filament nomenclature. Since ever more filament systems with new architectures are being. ... CM (2014) Subpolar addition of new cell wall is directed by DivIVA in.

11 Oct 2007: We propose a new model in whichlateral interactions help determine the curvature of protofilaments. ... Similarly, our new pair of AaFtsZ:GDPand AaFtsZ:8-morpholino-GTP show even smallerdifferences (rmsd 0.2 Å).

22 Aug 2007: c, Changing hubs: clathrin is recruited andpolymerization stabilizes the forming vesicle, displacing the AP2recruitment hub and becoming the new organizing hub. ... Clustered hubs’ are a new sub-type of hubs not previously described (Supplementary

14 Nov 2021: The new model containing a coiled-coil. a-helix describes map 2 well. ... Hagen WJH, Lindahl E and Scheres SHW (2018) New. tools for automated high-resolution cryo-EM structure.

21 Oct 2010: The identificationof quadruple as well as double filaments posed a new question,however: Which filament is present in cells? ... Both formed filaments similar to those of native TubZ. We then reconstructed the new filaments in three dimensionsto reveal

17 Mar 2003: cells. After induction with IPTG for 4 hr at 378C, cells were a new member of the AP-3 clathrin assembly protein family. ... Nature 347,N.S., et al. (1998). Crystallography and NMR system: a new software402–406.suite for macromolecular structure