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mbar-v2
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/model-building-and-refinement-v2.pdf31 Aug 2018: proceed. 2.2.3.The new waters have been added to chain D, and it is important to inspect them and make sure that they have all been added in appropriate -
ANALYSIS Integrating molecular and network biologyto decode…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007.pdf22 Aug 2007: c, Changing hubs: clathrin is recruited andpolymerization stabilizes the forming vesicle, displacing the AP2recruitment hub and becoming the new organizing hub. ... Clustered hubs’ are a new sub-type of hubs not previously described (Supplementary -
Cryo‐EM structure of the full‐length Lon protease from Thermus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf14 Nov 2021: The new model containing a coiled-coil. a-helix describes map 2 well. ... Hagen WJH, Lindahl E and Scheres SHW (2018) New. tools for automated high-resolution cryo-EM structure. -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/stock2005robot.pdf19 Jan 2005: authors. Thesedevelopments along with advances in structure determination techniques (mainly the use ofsynchrotrons, area detectors and crystal freezing) led to an enormous increase in new proteinstructures: in 1990, about 500 structures -
201010176 1..6
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Aylett-TubZ.pdf21 Oct 2010: The identificationof quadruple as well as double filaments posed a new question,however: Which filament is present in cells? ... Both formed filaments similar to those of native TubZ. We then reconstructed the new filaments in three dimensionsto reveal -
x16412u141
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MaoAP180-2001.pdf17 Mar 2003: cells. After induction with IPTG for 4 hr at 378C, cells were a new member of the AP-3 clathrin assembly protein family. ... Nature 347,N.S., et al. (1998). Crystallography and NMR system: a new software402–406.suite for macromolecular structure -
Dynamin-dependent and dynamin-independentprocesses contribute to the…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Graham02.pdf29 Apr 2002: a new mean half-width. -
doi:10.1016/j.jmb.2007.08.056
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Oliva_FtsZ_JMB_2007.pdf11 Oct 2007: We propose a new model in whichlateral interactions help determine the curvature of protofilaments. ... Similarly, our new pair of AaFtsZ:GDPand AaFtsZ:8-morpholino-GTP show even smallerdifferences (rmsd 0.2 Å). -
Tutorial 4: Statistical Theory;Why is the Gaussian Distribution so ...
https://www2.mrc-lmb.cam.ac.uk/download/tutorial-4-statistical-theory/?wpdmdl=18816&refresh=66a11b202a368172183427220 Mar 2014: 7. Use a ‘for loop’ to iteratively recreate a new random Bin(100, 0.4) sample of100 observations and overplot the corresponding empirical density functionsonto the plot of the Normal density -
Bb9e55.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Vallis_McMahon1999.pdf21 Mar 2003: of dynamin at a single site that defines a new SH3binding consensus sequence. -
Calcium-mediated Evidence from the Changes in Gap Junction Low ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb83.pdf1 Nov 2012: St. Louis, MO); sodium deoxycholate from Calbiochem-Behring Corp. (La Jolla, CA); and HLlabeled sodium deoxycholate (4 Ci/mmol) from New England Nuclear (Boston, MA). -
DOI: 10.1126/science.1256917, 963 (2014);346 Science et al.Thomas G.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Science-2014-Gligoris-963-7.pdf21 Nov 2014: The title. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. ... Cross-linking cohesin containing cysteinesat all three interfaces generated a new formform (Ci) whose electrophoretic mobility -
se060101047p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Itoh%20et%20al.pdf23 Nov 2004: X-ray Crystallography and NMR (Yale Univ. Press,New Haven, CT, 1993). 24. -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: LeoA, B and C from Enterotoxigenic Escherichia coli(ETEC) Are Bacterial DynaminsKatharine A. Michie1.¤a, Anders Boysen2., Harry H. Low1.¤b, Jakob Møller-Jensen2, Jan Löwe1. 1 MRC Laboratory of Molecular Biology, Structural Studies Division, -
Biol. Chem., Vol. 381, pp. 993 – 999, September/October ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/BiolChem%20Helical%20Tubes.pdf13 Sep 2000: Biol. Chem., Vol. 381, pp. 993 – 999, September/October 2000 Copyright by Walter de Gruyter Berlin New York. -
Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: References. Adams PD, Grosse-Kunstleve RW, Hung LW, Ioerger TR, McCoy AJ,Moriarty NW, Read RJ, Sacchettini JC, Sauter NK, Terwilliger TC(2002) PHENIX: building new software for automated crystal-lographic ... Mol Microbiol 51:1321–1332. Gerdes K (2009) -
DOI: 10.1126/science.1135394 , 239 (2007); 317Science et al.Nabil…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17626883.pdf8 Dec 2007: The title. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/download/molecular-replacement/?wpdmdl=18799&refresh=66a11b1be12ec172183426711 Jun 2013: Molecular replacement. New structures from old. The Phase Problem. amplitude. phase. -
Contrasting Membrane Interaction Mechanisms of AP180N-terminal…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Stahelin_cho2003.pdf16 Jul 2003: the Department ofMicrobiology and Immunology, Weill Medical College of Cornell University, New York, New York 10021, andthe Howard Hughes Medical Institute and Department of Cell Biology, Yale University School of ... Karol Bruzik.Phospholipid -
PII: 0896-6273(89)90235-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/neuron89.pdf11 Sep 2003: New channel proteins are being discovered and characterized, Superfamilies of voltage- and ligand-gated channels are being identified and ex- tended. ... without the energetically unfavored pro- cesses of creating substantial new surfaces or invoking -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: 3), requiring RsaA to adoptslightly different conformations. Furthermore, gaps in the latticewill be needed for insertion of new RsaA molecules on growingcells. ... The new culture was grown at 30 C for2–3 days. The culture was imaged using a -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: CONTENT ALERTS. more>>article), Receive: RSS Feeds, eTOCs, free email alerts (when new articles cite this. -
Bacterial actin: architecture of the ParMRC plasmidDNA partitioning…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ParMRC%20Salje%202008%20EMBO.pdf4 Aug 2008: ParM filaments while allowing entry of new ATP-bound. monomers. We propose a processive mechanism by which. ... The binding of a new subunit. induces ATP hydrolysis in the previous subunit of the proto-. -
Supplementary Text & Figs
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007Sup.pdf22 Aug 2007: be appropriately interpreted. Part 3: Clustered-hubs. Clustered-hubs are a new subtype of hubs not previously described. ... new subtype of hubs that we find in CME. These can be composed of proteins with. -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: protein droplets (each row now requires 8 new microsyringesto avoid cross-contamination). -
MeganPalm
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/MeganPalm.pdf3 Dec 2005: to find out new search regions to detect all the eggs attached to the worm body. ... previously used for the egg detection test and 20 new normal wild type videos. -
Many bacterial plasmids are maintained at low copy number ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nrmicro2425.pdf14 Sep 2010: Filaments stabilized in this way continue to polymerize with the addition of new ParM subunits at both ParR–parC-capped ends, although the actual rate of elongation of an individual -
©20 10 N atur e A mer ica, Inc. ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20081827.pdf2 Aug 2010: We devised a new expression and purification strategy by introducing a TEV protease. ... All propeller-shaped inhibitors create a new specificity pocket not present in the apo form of the enzyme. -
Activation of GCN2 by the ribosomal P-stalk
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/PNAS_2019.pdf7 Mar 2019: New insight into a possible functional link be-tween GCN2 and ribosomes came from a recent analysis of micelacking both a specific neuronal tRNA (tRNAArgUCU) and theputative ribosome recycling factor GTPBP2 -
The MORPHEUS protein crystallization screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/morpheus%202009.pdf12 Nov 2009: LMB). The concept integrates several innovative approaches,such as chemically compatible mixes of potential ligands, new buffer systems andprecipitant mixes that also act as cryoprotectants. ... Both assumptions were a driving force behind my attemptsto -
Filament structure and subcellular organization of the bacterial…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Liu_PNAS_crescentin_2024.pdf28 Apr 2024: contributed new reagents/analytic tools; Y.L. and J.L. analyzed data; and Y.L. -
740 Research Paper Calcium triggers calcineurin-dependent synaptic…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Marks_McMahon1998.pdf21 Mar 2003: We interpret theseresults to indicate that cyclosporin A is inhibiting theendocytic formation of new synaptic vesicles that normallyoccurs when the synaptosomes are depolarised. ... a single site that defines a new SH3 bindingconsensus sequence. -
Proc. Natl. Acad. Sci. USAVol. 94, pp. 9637–9642, September ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9275175.pdf20 Apr 2002: Brünger, A. (1992) X-PLOR Manual (Yale Univ., New Haven,. CT).26. Laskowski, R. -
FtsK in motion reveals its mechanism for double-stranded DNA…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK_Jean_2020_PNAS.pdf8 Jun 2020: J. Begg, S. J. Dewar, W. D. Donachie, A new Escherichia coli cell division gene, ftsK. ... Nucleic Acids Res. 32, 5260–5279 (2004). 28. J. Zivanov et al., New tools for automated high-resolution cryo-EM structure de-termination in RELION-3. -
Proteins containing photosynthetic reaction centre domains modulate…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Nussbaum_Nat_micro_2024.pdf1 Mar 2024: All enzymes for DNA amplification (Phusion Polymerase) and plasmid construction (restriction enzymes, T4 DNA ligase and Quick CIP) were obtained from New England Biolabs (NEB) and used according to the -
Prokaryotic cytoskeletons: protein filaments organizing small cells
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nrmicro.2017.153.pdf18 Jan 2018: as well as entirely new classes of filaments16–18, leading to an expanded concept of what a cytoskeleton is (BOX 1). ... In many rod‑shaped bacteria, dynamic MreB filaments control the synthesis of a new cell wall to maintain shape. -
PII: S0166-2236(98)01264-8
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Wigge_McMahon1998.pdf8 Jul 1998: 1997) J. Biol. Chem. 272, 11629–1163524 Folli, F. et al. (1993) New Engl. -
The patterned assembly and stepwise Vps4-mediated disassembly of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf16 Jul 2023: This makes itpossible to visualize new DNA synthesis through the incorporationof the thymidine analog EdU by fluorescence microscopy (29). ... Fragmentswere cloned into the pSVAaraFX backbone for Vps4E209Q and thepSVAaraHA-stop backbone for CdvBΔMIM2, -
Crystal Structure of the SMC Head Domain: An ABC ATPase with 900…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TmSMChd%20JMB%202001.pdf20 Feb 2001: L. (1998).Crystallography and NMR system: a new softwaresuite for macromolecular structure determination.Acta Crystallog. -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/MR_LMB.pdf11 Jun 2013: Molecular replacement. New structures from old. The Phase Problem. amplitude. phase. -
Features critical for membrane binding revealed by DivIVA crystal…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf20 May 2010: Corresponding authors. LW Hamoen, The Centre for Bacterial CellBiology, Medical Science New Building, Medical School, NewcastleUniversity, Richardson Road, Newcastle upon Tyne NE2 4AX, UK.Tel.: þ44 191 208 3240; Fax: þ44 ... These were then removed -
30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf1 May 2004: The SulA fold is related to a fragment of RecA, but this seems accidental andprobably only shows how nature reuses successful designs for new purposes. ... For GFP labeling, new genetic tools in mollicutes must be devel-oped for that purpose. -
The structure of bactofilin filaments reveals their mode of membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf26 Aug 2019: from genomic DNA and cloned into vector pET15b using Gibson assembly (New England Biolabs). ... plasmid pHis17 using Gibson assembly (New England Biolabs) with a stop codon before the C-terminal tag on the plasmid. -
doi:10.1016/j.biocel.2008.08.010
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cytomotive2008Lowe.pdf17 Sep 2008: The adaptor protein ParR has ‘formin-like activity’, meaning. t actively participates in the addition of new ParM subunits to thend. -
mmi_4133.fm
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Spo0J%202004.pdf30 Jun 2004: Molecular Microbiology (2004). 53. (2), 419–432 doi:10.1111/j.1365-2958.2004.04133.x. 2004 Blackwell Publishing Ltd. Blackwell Science, LtdOxford, UKMMIMolecular Microbiology0950-382XBlackwell Publishing Ltd, 2004? 2004. 53. 2419432. Original -
Current Developments in Coot for Macromolecular Model Building…
https://www2.mrc-lmb.cam.ac.uk/coot/protein-science/Casanal_Lohkamp_Emsley_11112019-pe-2.pdf11 Nov 2019: Faster refinement goes hand in hand with the addition of new restraint types. ... library. The new mode of Acedrg allows the generation of bespoke chemical links,. -
Crystal Structure of the Two N-terminal Domains of g3p from…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10329170.pdf6 May 1999: digested with SI and NotI(New England Biolabs), and ligated into the SI and NotIdigested pUC119His6 (Holliger & Riechmann, 1997). ... Filamentous bacterio-phages. In The Bacteriophages (Calendar, R., ed.), pp.375-456, Plenum Press, New York. -
DOI: 10.1126/science.1229091, 1334 (2012);338 Science et al.P.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/sciencegayathri2012/Science-2012-Gayathri-1334-7.pdf7 Dec 2012: The title. CopyrightAmerican Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. ... new,higher steady state. -
BIO CHEM ISTR Y Structure of the SARS-CoV-2 RNA-dependent ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SARS%20polym%202021%20e2021946118.full.pdf29 Aug 2021: High-resolution structure deter-mination may aid in these directions as new compounds are developed andtested as treatments for COVID-19. ... 9, 2. 021. 20. J. Zivanov et al., New tools for automated high-resolution cryo-EM structuredetermination in -
INVITED REVIEW Mechanosensory molecules and circuits in C. elegans ...
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-2.pdf10 Dec 2014: INVITED REVIEW. Mechanosensory molecules and circuits in C. elegans. William R. Schafer. Received: 3 June 2014 /Revised: 30 June 2014 /Accepted: 2 July 2014 /Published online: 23 July 2014# The Author(s) 2014. This article is published with open
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