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1 JED: Questions Arising ● Does the Acedrg new ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/JED-questions-arising.pdf30 Sep 2017: i.e., use the same description and apply it to the new ligand in question, just like for sugar links. ... generated by Acedrg's new mode). We don't have anything new to offer for modifications. -
Pymol guide
https://www2.mrc-lmb.cam.ac.uk/groups/nagai/download/pymolguide.pdf19 Dec 2017: So if you click on (sele) you can toggle and untoggle your new selection. ... So if you want to make a new selection, make sure you deselect everything first! -
6. Image refinement Sjors Scheres EM course 2017 Agenda ...
https://www2.mrc-lmb.cam.ac.uk/download/cryo-em17-lecture-06-refinement/?wpdmdl=18760&refresh=668ff5b2bbbd317207105784 Oct 2017: calculate new 2D average as probability weighted averages. Reference-free 2D class averaging. ... The bad news • The experimental data alone is not enough to determine a unique solu7on! • -
Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf29 May 2017: Added into a new N-linked tree: – paucimannose. 23/40. Xyl-β1,2. Xyl - β1,2 – Man – using XYP (beta D xylosepyranose). – ... both GUI interface and built into the auto-builder. New Interface. -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: 3), requiring RsaA to adoptslightly different conformations. Furthermore, gaps in the latticewill be needed for insertion of new RsaA molecules on growingcells. ... The new culture was grown at 30 C for2–3 days. The culture was imaged using a -
ligand-fitting-tutorial.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ligand-fitting-tutorial.pdf24 Oct 2017: The centre of the screen is a bit crowded now. Undisplay those new ligands. ... When we are happy with our fit, we can merge this new ligand into the mainprotein ligand as we did before. -
Fei Long MRC Laboratory of Molecular Biology, Cambridge, U.K. ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Ligand_meeting_Sept_29_2017.pdf29 Sep 2017: new_atom_id _chem_mod_atom.new_type_symbol _chem_mod_atom.new_type_energy _chem_mod_atom.new_charge TMPmod1 delete H2. ... chem_mod_bond.new_type _chem_mod_bond.new_value_dist _chem_mod_bond.new_value_dist_esd TMPmod1 delete C1 H2 single. -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: dejongeii DSM 12251 (PdBtubAB), a stop codon (TAA)was introduced before the C-terminal hexahistidine tag in the BtubAB bacterialT7 expression plasmid used (7) by Q5 mutagenesis (New England Biolabs). ... E5954 | -
1. A historical perspective and course overview
https://www2.mrc-lmb.cam.ac.uk/download/image-formation-fourier-analysis-and-ctf-theory-paula-da-fonseca/?wpdmdl=18758&refresh=668ff5b282522172071057830 Jun 2017: Carter, C.B. Transmission electron microscopy (Plenum Press, New York, 1996). • -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: These constructs are in a vector (provided with the PURE system kit from New England Biolabs) that contains a T7 promoter and transcriptional terminator. ... The resin was washed four times with 1 ml PSB2 and transferred to a new tube after the fourth -
A Polymerization-Associated Structural Switch in FtsZ That Enables…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf3 May 2017: the divisome around the division site (17, 18).These findings have resurrected an old model of bacterial cell division: the templatemodel, in which the closing septum constricts by new cell wall ... because the energetic cost of losing subunits from ends -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: using the Gibson Assembly kit (New England Biolabs). -
REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf19 Jan 2017: PURE system translation reactions. Initial experiments used the PURE translation system from New England Biolabs as per the manufacturer’s instructions. -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf22 Sep 2017: authors. Thesedevelopments along with advances in structure determination techniques (mainly the use ofsynchrotrons, area detectors and crystal freezing) led to an enormous increase in new proteinstructures: in 1990, about 500 structures
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