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23 Dec 2013: coli cells, we constructed C-terminal fusions of the fluorescentprotein YPet to wild-type FtsK and truncated derivatives of it ex-pressed from the endogenous wild-type chromosomal locus.

6 Dec 2014: Unfortunately, it is currently not possible to reliablyimage at endogenous levels of MinC with SIM.

23 Nov 2004: Endogenous SNX1 was enriched on tubularmembranes in close vicinity to endosomal vacuoles (Fig-ure 3). ... These data establish a role for endogenous SNX1in the retrieval of the CI-MPR from early endosomes tothe TGN.

6 Oct 2004: The challenge now is to establish the molecularhave similar phenotypes: they retain a similar shape tolinks between endogenous ARAP3’s control of Rho andcontrol cells, but on PDGF stimulation their lamellipodiaArf

14 Jun 2007: How do various ESCRT components link to other processes such as pH regulation of gene expression [51] or retrovirus budding [52]?

27 Mar 2012: away from the nucleotide-binding site of FtsA (Figure 2A,. right), which contains an endogenous ATP molecule at. ... appearance that is straightforward to distinguish and. quantify. We then complemented the endogenous ts-FtsA.

3 Feb 1997: . The Rockefeller University Press, 0021-9525/97/02/567/15 $2.00The Journal of Cell Biology, Volume 136, Number 3, February 10, 1997 567–581 567. A Multistep, ATP-dependent Pathway for Assembly ofHuman Immunodeficiency Virus Capsids in a

27 Apr 2009: However, the endocytosisof many other types of endogenous and exoge-nous cargoes is relatively unaffected by inhibi-tion of this pathway. ... Relatively few endogenous, noncargo pro-teins associated with clathrin-independent. endocytic pathways have been

23 Nov 2004: The puncta. begin to appear at temperatures below 181C and colocalisewith endogenous AP2. ... 6C. 6C. 37C. Endogenous AP2Overexpressed epsins Transferrin uptakeA. B. C (d) Coated vesicle assemblyClathrin.

25 Jun 2007: Myc-EpsinR D34G R67L with endogenous AP1 and internalised transferrin in COS cells.

20 Apr 2015: Published on. 20 April, 2015. The existence of an endogenous daily clock in humans is well known: it is what drives the 24-hour sleep/wake rhythm to match the daily

21 Jul 2023: Generation and live imaging of an endogenous Cdx2 reporter mouse line..

15 Nov 2011: To evaluatePtdIns(4,5)P2 content in the plasma membrane and to competefor PtdIns(4,5)P2 binding with endogenous PtdIns(4,5)P2-bindingproteins, we expressed PLCδ1PH (PH domain of ... Lowering the concentrations of PtdIns(4,5)P2available for interactions

13 Apr 2015: Totest this, we analyzed the interactions between Sec61band endogenous TRC40 versus exogenously added (andepitope-tagged) TRC40-DN. ... with release from endogenous TRC40. 1156 Cell 128, 1147–1159, March 23, 2007 ª2007 Elsevier Inc.

31 Jan 2020: rTG appears to be indistinguishable by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and cryo-electron microscopy (cryo-EM) from endogenous TG (eTG) puri-fied from thyroid glands of patients

2 Nov 2002: Endogenous EpsinR. Overexpressed. Myc-Epsin1. High levels of overexpression lead to grossly enlarged perinuclear compartments These compartments colabel with M6P receptors and TGN46 but do not co-label with endogenous GM130

26 Sep 2011: For a detailed study of endogenous epsinR and overexpression of wild-type and mutants in cells see immunocytochemistry.

20 Jan 2022: Published on. 20 January, 2022. A schematic showing the effect of CRY1 on the relationships between localisation and intracellular mobility of endogenous fluorescently tagged PER2 as well as circadian rhythmicity of ... Real-time imaging of endogenous

4 Mar 2019: Articleshttps://doi.org/10.1038/s41594-019-0196-z. 1MRC Laboratory of Molecular Biology, Cambridge, UK. 2Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK. 3Bioanalytics, Institute of Biotechnology, Technische

6 Jun 2014: elifesciences.org. van den Ent et al. eLife 2014;3:e02634. DOI: 10.7554/eLife.02634 1 of 22. Bacterial actin MreB forms antiparallel double filamentsFusinita van den Ent1†, Thierry Izoré1†, Tanmay AM Bharat1, Christopher M Johnson2, Jan Löwe1.

10 Jan 2013: siRNA of endogenous protein led to decreased cell migration, increasedphagocytosis and reduced podosome density and dynamics. ... Both endogenous and overexpressed Bin2-EGFP. localized to ring-like structures around the actin core of.

30 Jul 2013: inherent flexibility of the tubes and other factors, such as heterogeneity of the endogenous.

21 Nov 2014: Toaddress whether cohesin containing Smc3R61Qloads onto chromosomes, we compared the dis-tribution of green fluorescent protein (GFP)–tagged Smc3R61Q and wild-type Smc3 in livingcells carrying an untagged endogenous Smc3gene.

30 Apr 1999: and endogenous rat brain vesicles. J. Biol. Chem. 268, 17240–17246 (1993).

16 Feb 2010: 2007). To maximize the severity of any phenotype, PrP+/ mice were used because endogenous PrP was observed in earlier studies to exacerbate CtmPrP-mediated neurodegeneration (Stewart et al., 2005).

16 Jul 2008: SDS-PAGE, andimmunoblotted using an antibody (3F4) that specifically detects transfected but not endogenous PrP. ... C) Biosynthesisand maturation of endogenous PrP in A4 and L-cells were assessedby pulse-chase analysis followed by immunoprecipitation

24 Jan 2009: It remains tobe determined whether endogenous Syt-1 regulates dense-core vesicle fusion pore expansion and if so, whether Syt-1utilizes membrane binding and curvature induction, or in-teractions with SNAREs, ... Syt Effector Interactions Regulate Fusion

30 Apr 2010: Itdoes, however, stimulate the translation of some proteins(presumably because the endogenous levels of some tRNAsin the RRL are limiting), and it is possible that some RRLpreparations contain relatively low amounts ... 5. Endogenous RRL usually contains

8 Nov 2004: Endogenous NF-kB (indicated by an aster-isk) serves as a loading control. ... Immunoblots were probed with antibodiesagainst PrP, GFP, and an endogenous protein, the beta subunit ofSec61.

25 Nov 2019: Nature Structural & Molecular Biology, doi:10.1038/s41594-019-0331-x

18 Feb 2016: Ribosome profiling in the presence and absence of Dom34 has shown that an endogenous substrate for this pathway is Hac1, a cytosolically spliced mRNA whose incorrect ligation results in an mRNA ... The key open questions include the following: (i) What

29 May 2019: For correspondence:. rhegde@mrc-lmb.cam.ac.uk. Competing interest: See. page 28. Funding: See page 28. Received: 11 March 2019. Accepted: 15 May 2019. Published: 16 May 2019. Reviewing editor: Maya. Schuldiner, Weizmann Institute,. Israel. Copyright

14 Dec 2010: amount of endogenous ACh at the synapse by preventing its metabolism by AChE. ... communication). 8. The endogenous agonist, serotonin, is a stable compound but was thought not.

16 Jul 2023: 1A), hereafter calledVps4E209Q. This point mutation prevents AAA-ATPases like Vps4from hydrolyzing bound ATP and can therefore interfere with theactivity of endogenous Vps4 complexes when overexpressed (24,28).

19 Nov 2019: Cite as: Z. Lin et al., Science 10.1126/science.aaz4352 (2019). REPORTS. First release: 14 November 2019 www.sciencemag.org (Page numbers not final at time of first release) 1. Alpha and beta tubulins form obligate heterodimers (hereaf-ter

14 Jul 2020: Approximately 24h endogenous biological oscillation that persists under constant conditions, whose period is temperature compensated. ... Approximately 24h endogenous biological oscillation that persists under constant conditions, whose period is

17 Jan 2012: Instead, both endogenous and overexpressed GRAF1 were found enriched at small, round, vincu-lin-positive structures in a subset of cells (see Figure S1, C and D). ... Cells were acid-stripped and fixed and were then labeled for endogenous paxillin.

26 Aug 2021: Othersubunits of the 19S base were copurified atlower levels, consistent with partial assemblyof PSMC5 with endogenous base subunits pres-ent inthe lysate (table S5). ... Boosting HERC1 levelsusing CRISPR activation (31) of the endoge-nous HERC1 promoter

24 Aug 2011: By Western blot analysis both endogenous and transfected Flot1 and GRAF1 were concentrated in Fraction 2.8 (Fig. ... 4 G, arrowheads). Endogenous dynamin labeled a significant population of struc-tures within Fraction 2.8 (Fig.

11 Aug 2015: By contrast, endogenous SRP in a homologous mammalian system hasa remarkably subtle effect on translation elongation (Wolin and Walter, 1989). ... Here we demonstrate that endogenous SRP is efficiently recruited to mammalian ribosomescontaining a

9 May 2003: endogenous proteins and quenching of signals by other cellular proteins. This discussion.

10 Oct 1997: We interpret these results tomean that either Amph1 or Amph2 overexpressionalone leads to sequestration of endogenous dynamininto inactive complexes, but when the two proteins areexpressed together, forming a heterodimer, endocyto-sis

18 Oct 2008: a high concentration of an endogenous PDZ-binding. Figure 5: The C-termini of GluR2 and of DAT fused to thesingle transmembrane protein Tac are sufficient for BARdomain-dependent coclustering with ... This. juxtanuclear clustering strongly resembles the

21 Feb 2011: Previous characterization ofBAR domain proteins has generally involved overproduc-tion, raising the need for in vivo studies characterizing theconsequences of BAR domain mutations in genes expressedat their endogenous level. ... these alterations affect

2 Aug 2010: 26]. The p101 subunitprotects cells from apoptosis when overexpressed, and thismay explain why it is a common site for insertion in T-celllymphomas induced by the MoFe2-MuLV retrovirus [27].The

5 Aug 2013: Second, the system is typicallyorthogonal to endogenous pathways and utilizes a single definedligase. ... Furthermore, the absolute dependence on phospho-Vpu illustrates that endogenous ligases in this system do notperceive mCD4 as a misfolded protein

7 Oct 2005: he same for both Crt and Prl-Crt and were comparableo the levels of endogenous Crt (Figure 5A). ... A) Crt/ cells stably expressing either Crt-GFP or Prl-Crt-GFPwere analyzed (in duplicate) by immunoblotting with anti-Crt andanti-Sec61β (an endogenous

5 Jan 2016: ribosomes used for the cryo-tomography structure (16), thepresence or absence of heterogeneous endogenous nascentpolypeptides within Sec61 could not be determined conclusively.Thus, the conclusion that the Sec61 complex is constitutivelyopen

9 Sep 1998: We believe this effectis due to sequestration of endogenous dynamin away fromclathrin-coated pits.

11 Aug 2015: Because stable association of NEMF with 60S requires a peptidyl-tRNA (Shao et al., 2015), these complexes are likely to contain endogenous nascent chains. ... We examined this idea by analysis of endogenous RNCs on ER microsomes isolated from native