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  2. Effects of Epsin R overexpression

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/epsinrL10E.html
    25 Jun 2007: Myc-EpsinR L10E with endogenous AP1 and internalised transferrin in COS cells.
  3. How eating feeds into the body clock - MRC Laboratory of Molecular…

    https://www2.mrc-lmb.cam.ac.uk/how-eating-feeds-into-the-body-clock/
    Thumbnail for How eating feeds into the body clock - MRC Laboratory of Molecular Biology 25 Apr 2019: These are endogenous daily rhythms that occur in every cell of the body; affecting a wide range of physiological processes, from when we sleep, to hormone levels, to how quickly we
  4. Epsin 1 colocalization with endocytic markers

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/IF/E1coloc.html
    25 Jun 2007: Colocalisation of the wild type epsin (green) with endogenous clathrin (X22), Eps15 (Ra15), dynamin (McNiven antibody) and AP1 (100.3).
  5. Discovering the origins of biological timekeeping - MRC Laboratory of …

    https://www2.mrc-lmb.cam.ac.uk/discovering-the-origins-of-biological-timekeeping/
    Thumbnail for Discovering the origins of biological timekeeping - MRC Laboratory of Molecular Biology 20 Apr 2015: Published on. 20 April, 2015. The existence of an endogenous daily clock in humans is well known: it is what drives the 24-hour sleep/wake rhythm to match the daily
  6. Kate McDole - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/group-leaders/h-to-m/kate-mcdole/
    Thumbnail for Kate McDole - MRC Laboratory of Molecular Biology 21 Jul 2023: Generation and live imaging of an endogenous Cdx2 reporter mouse line..
  7. HIV uses capsid pores to import nucleotides and evade innate immunity …

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Fhiv-uses-capsid-pores-import-nucleotides-evade-innate-immunity%2F&format=xml
    mrc-lmb.cam.ac.uk/wordpress/wp-content/uploads/Leo-James-HIV-Nature-cover-28-July-16.jpg 700 700 HIV is a retrovirus, meaning it has to copy its RNA
  8. https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/9259

    https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/9259
    HIV is a retrovirus, meaning it has to copy its RNA genome into DNA in order to infect cells.
  9. Early transcriptional patterning of a forming tissue is essential to…

    https://www2.mrc-lmb.cam.ac.uk/early-transcriptional-patterning-of-a-forming-tissue-is-essential-to-morphogenesis-of-a-tubular-organ/
    Thumbnail for Early transcriptional patterning of a forming tissue is essential to morphogenesis of a tubular organ - MRC Laboratory of Molecular Biology 8 Nov 2021: Yara Sanchez-Corrales, a postdoc in Katja’s lab, tagged endogenous proteins to label cell shapes in Drosophila embryos.
  10. doi:10.1016/j.cub.2004.09.077

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Snx1PeteCullen2004/Snx1PeteCullen2004.pdf
    23 Nov 2004: Endogenous SNX1 was enriched on tubularmembranes in close vicinity to endosomal vacuoles (Fig-ure 3). ... These data establish a role for endogenous SNX1in the retrieval of the CI-MPR from early endosomes tothe TGN.
  11. Decade-long collaboration results in the first structure of the…

    https://www2.mrc-lmb.cam.ac.uk/decade-long-collaboration-results-in-the-first-structure-of-the-fanconi-anaemia-core-complex/
    Thumbnail for Decade-long collaboration results in the first structure of the Fanconi anaemia core complex - MRC Laboratory of Molecular Biology 31 Oct 2019: to exogenous chemicals, such as chemotherapeutic drugs and alcohol, or endogenous by-products of normal cellular metabolism.
  12. A positively charged channel within the Smc1/Smc3 hinge required for…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf
    1 Dec 2010: together with endogenous Smc3. Western blotting revealed. that immunoprecipitates of Smc3-HA3 or Smc3AAA-HA3. ... As expected, similar amounts of Smc1-myc9 and endogenous. Smc1 associate with Smc3-HA3.
  13. The ER membrane protein complex is a transmembrane domain insertase

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017c.pdf
    27 Jan 2018: This system contains endogenous mRNAs (primarily encoding the 14 kD globins and a prominent 70 kD protein) that contribute to background, but has the advantage of retaining native cytosolic conditions, most ... aureus is used to digest endogenous mRNA.
  14. Articleshttps://doi.org/10.1038/s41594-019-0196-z A folded…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%20NSMB%202019%20SI.pdf
    26 Feb 2019: c, Growth of strains containing point mutations at the elbow in the endogenous mukB gene.
  15. 1256917combinedsupplement

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gligoris-SM.pdf
    14 Nov 2014: Scc1’s endogenous M1 has been deleted, an N-terminal myc9 epitope was fused in frame and endogenous M30 was changed to I30. ... Endogenous Scc1M102, Smc3M139 and M186 generate a 30 kDa Scc1 and a 5 kDa Smc3 fragment.
  16. Bb9e55.qxd

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Vallis_McMahon1999.pdf
    21 Mar 2003: Mol Biol Cell 1998, 9:195a. 22. Tuma PL, Stachniak MC, Collins CA: Activation of dynamin GTPaseby acidic phospholipids and endogenous rat brain vesicles. ... such adynaminPH construct into COS cells will be even betterat sequestering endogenous dynamin
  17. Cryptochrome proteins are integral to maintain time within the…

    https://www2.mrc-lmb.cam.ac.uk/cryptochrome-proteins-are-integral-to-maintain-time-within-the-brains-master-clock/
    Thumbnail for Cryptochrome proteins are integral to maintain time within the brain’s master clock - MRC Laboratory of Molecular Biology 20 Jan 2022: Published on. 20 January, 2022. A schematic showing the effect of CRY1 on the relationships between localisation and intracellular mobility of endogenous fluorescently tagged PER2 as well as circadian rhythmicity of ... Real-time imaging of endogenous
  18. doi:10.1016/j.devcel.2008.06.015

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/54_Rane_NS_DevCell_2008.pdf
    18 Sep 2008: Ifn-PrP. Note the lack of changes to endogenous PrP in cells expressing. ... Thus, in both cases, Ifn-PrP is not being overexpressed relative. to endogenous PrP.
  19. © 2005 Nature Publishing Group Endophilin and CtBP/BARS are ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndopohilinNature2005.pdf
    4 Jan 2006: A JC201 strain ofEscherichia coli lacking endogenous LPAAT activity grows at 30 8Cbut not at 42 8C (ref. ... coli that has no endogenous LPAATactivity (JC201 strain) cannot growat 42 8C (sector 1).
  20. pq240013132p

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/16_Wilhelm_JE_PNAS_2000.pdf
    10 Nov 2000: 1B), despitethe fact that endogenous Vg1 was expressed only in the late-stageoocytes (Fig. ... 1C). This result raisesthe possibility that the endogenous, localized Vg1 message is inan environment that is free from repression.
  21. TBS may template.qxd

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/EMreview.pdf
    3 May 2002: This might be caused bycompetition for clathrin binding between the injectedpeptide and the endogenous protein, therebypreventing endogenous AP180 and AP2 frompromoting clathrin assembly at the plasmamembrane.
  22. Cooperative Recruitment of Dynamin and BIN/Amphiphysin/Rvs (BAR)…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/JBC_Meinecke2013.pdf
    19 Mar 2013: Amph12 RNAi) depletion on the recruitment of endogenous dynamin 2 (dynaminen, green and white arrows) and clathrin light chain A (clathrinen, red). ... Bar, 10µm (B) Effect of SNX9, amphiphysin and endophilin overexpression on endogenous dynamin 2
  23. Membrane curvature sensing and induction: The function of BAR domains …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/BARdomains/BARsupp.pdf
    20 Nov 2003: S6. Colocalization of wild type, mutant, and endogenous arfaptin with TGN46 Table S1.
  24. doi:10.1016/j.molcel.2004.08.030

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf
    20 Sep 2004: Cells separatedsister chromatids with identical kinetics to those thatof the GAL1-10 promoter in cells with an endogenous. ... Nevertheless, noncleavableScc1-HA3 from the endogenous SCC1 promoter. The. sole copy of Cdc20 in all three strains was
  25. The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf
    28 Nov 2008: G) Epifluorescence micrographs of a HeLa cell incubated with CTxB for 5 min before fixation and staining for endogenous GRAF1. ... B, Confocal micrograph of an NIH 3T3 cell stained for endogenous GRAF1 distribution.
  26. Transmembrane Domain Modulates Sorting of MembraneProteins in…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/29_Karsten_V_JBC_2004.pdf
    2 Jun 2004: 1B).BAPGRA4 was localized to dense granules as evidenced bycolocalization with the endogenous dense granule proteinGRA3 (32). ... Both endogenous GRA4 andBAPGRA4 were efficiently translocated across the ER mem-brane (Fig.
  27. TBS may template.qxd

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/mcmahon.pdf
    3 May 2002: This might be caused bycompetition for clathrin binding between the injectedpeptide and the endogenous protein, therebypreventing endogenous AP180 and AP2 frompromoting clathrin assembly at the plasmamembrane.
  28. 53091 361..366

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf
    3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong.
  29. 554 Research Paper Inhibition of receptor-mediated endocytosis by the …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf
    21 Mar 2003: The most likely explanation for this is that theinteraction of dynamin with endogenous amphiphysin wasdisrupted in these cells. ... J Cell Biol 1993, 127:725-735. 18. Tuma PL, Stachniak MC, Collins CA: Activation of dynamin GTPaseby acidic phospholipids
  30. REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf
    4 Aug 2017: Catalytically inactive UBE2O(C1040S) alsorecovered endogenous and exogenous RPL24(but no detectable ubiquitinated forms), whereasUBE2O(DCR2) interacted poorly with RPL24 (fig.S10D). ... The heterogeneity of ubiquitin bands in the samples with cytosol is
  31. Features critical for membrane binding revealed by DivIVA crystal…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf
    20 May 2010: tionally complements an endogenous divIVA replacement. This was also seen when sporulation of these strains was. ... endogenous DivIVA through dimerization or oligomerization. When localization of wild-type DivIVA-GFP was analysed in.
  32. Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf
    4 Jan 2006: various endophilins and controls. a. b. c. Transformation of JC201 bacteria with endophilin does not lead to an acceleration of growth kinetics, unlike transformation of the endogenous LPAAT.
  33. tra_578 1..18

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Burtey%20IVF%20Traffic%2007.pdf
    15 May 2007: colocalizing with endogenous AP-2 complexes (Figure 2. G–J). In contrast, the IVA mutation resulted in complete. ... plasma membrane (Figure 5A), which colocalized with both. clathrin–DsRed and endogenous AP-2 complexes (Figure 5.
  34. The ER membrane protein complex promotes biogenesis of sterol-related …

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/JCS_2019.pdf
    18 Jan 2019: SQS insertion by EMC enables sterol biosynthesisTo determine the mechanism responsible for downregulating SQSin EMC-knockout cells, we monitored endogenous SQS synthesisand turnover by performing 35S-Met/Cys pulse-chase assays. ... A) 35S-Met/Cys
  35. Filament structure and subcellular organization of the bacterial…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Liu_PNAS_crescentin_2024.pdf
    28 Apr 2024: This yielded protein levels of CreS similar to endogenous levels in wt strain CB15N (SI Appendix, Fig. ... Subcellular Organization of CreS Filaments. The ultrastructure of endogenous CreS assemblies at normal levels in C.
  36. cm6307.qxd

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TAXOL.C&B.pdf
    18 Feb 1999: Such molecules mightmimic an endogenous cellular factor, presumably a lesspotent one, that helps to stabilise microtubules in normalcytoplasm; the natural reagent might be a small molecule orpart of a microtubule-associated
  37. A Ubiquitin Ligase-Associated Chaperone Holdase Maintains…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/72_Wang_Q_Mol_Cell_2011.pdf
    23 May 2012: IB, immunoblot-. ting. (B and C) Endogenous interactions between the. Bag6 and the gp78-p97 complexes. ... Trc35 Maintains a Cytosolic Pool of Bag6(A) Trc35 expression alters the subcellular localization of endogenous Bag6.
  38. Sensory Neurons Arouse C. elegans Locomotion via Both Glutamate and…

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-4.pdf
    30 Jun 2015: Under these conditions, we only observed endogenous acetylcholine EPSCs. To recordGABAergic postsynaptic currents, the holding potential was 0 mV, at which we only observemIPSCs. ... Supporting InformationS1 Fig. Stimulus-evoked EPSCs and endogenous
  39. No Job Name

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/vandenent2008ftsq.pdf
    4 Mar 2008: As shown in Fig. 5, WTFtsQ expressed together with sYFP1-FtsL in LMC531cells (that contain the endogenous temperature-sensitivemutation E125K) target FtsL to midcell at both 28C and42C (Fig. ... The resulting strains wereconfirmed by PCR analysis,
  40. Discovering the origins of biological timekeeping - MRC Laboratory of …

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Fdiscovering-the-origins-of-biological-timekeeping%2F&format=xml
    existence of an endogenous daily clock in humans is well known: it is what drives the 24-hour sleep/wake rhythm to match the daily cycle of night and.
  41. pone.0107211 1..10

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf
    3 Sep 2014: coli ETEC H10407 was investigated. The protein at endogenous expression levels is easilydetected and this signal disappears in a leoA KO strain, demonstrating specificity.
  42. doi:10.1016/j.ceb.2003.11.005

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cocb%202003.pdf
    27 Nov 2003: Immunofluorescence microscopy of endogenous Mbl in fixed cells gave. similar images.
  43. Molecular Biology of the CellVol. 18, 3681–3691, September 2007 ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/48_Rutkowski_DT_MBC_2007.pdf
    15 Aug 2007: ER stress did not alterthe electrophoretic migration or protease protection ofp58IPK (Figure 3A), suggesting that there is no substantialrelocalization of endogenous p58IPK during ER stress. ... These results demon-strate a specific interaction between ER
  44. 53091 361..366

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahon01020.pdf
    3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong.
  45. Functional Depletion of Mahogunin by Cytosolically Exposed Prion…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/59_Chakrabarti_O_Cell_2009.pdf
    13 Jun 2009: PrP would sufficiently influence endogenous Mgrn localization. to at least partially phenocopy a Mgrn depletion. ... Because endogenous Mgrn appears to normally. be associated tightly with membranes, we could not use selec-.
  46. Interaction of tau protein with the dynactincomplex Enrico…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBOJ2007-Magnani-Fan.pdf
    15 Oct 2007: A) Endogenous p150 co-immunopre-cipitates with tau in differentiated SH-SY5Y cells. Immunoprecipitation was performed with anti-tau antibody BR133 and immunoblots withp150 antibody. ... transgenic mice (data not shown) has been hampered by a. likely
  47. Cryo‐EM structure of the full‐length Lon protease from Thermus…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf
    14 Nov 2021: the ATP-dependent Lon protease- lessons from. endogenous protein and synthetic peptide substrates.
  48. Protein targeting and degradation are coupled for elimination of…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/73_Hessa_T_Nature_2011.pdf
    14 Jul 2011: endogenous ubiqui-tin was more than 90% competed out, resulting in few or no untagged ubiquiti-nated products. ... The absence of endogenous charged tRNAs and haemoglobinincreased photocrosslinker incorporation and photolysis, respectively.
  49. A Conserved Archaeal Pathway for TailAnchored Membrane Protein…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/70_Sherrill_J_Traffic_2011.pdf
    11 Nov 2011: We also examined whether archaeal TRC40 could func-tionally replace the endogenous mammalian TRC40 atphysiological concentrations. ... absence of endogenous mam-malian TRC40 using a phenyl- and Diethylaminoethyl (DEAE)-Sepharose-depleted RRL (PD-RRL)
  50. doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf
    23 Dec 2013: coli cells, we constructed C-terminal fusions of the fluorescentprotein YPet to wild-type FtsK and truncated derivatives of it ex-pressed from the endogenous wild-type chromosomal locus.
  51. ppat.1000479 1..19

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/60_Ashok_A_PLoS_Path_2009.pdf
    20 Jun 2009: completely unrelated human cell line that expresses very low (but. detectable) levels of endogenous PrP. ... presence or absence of endogenous PrP in co-expression. experiments (e.g., as in Fig.

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