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Protein labeling and imaging
https://www2.mrc-lmb.cam.ac.uk/ccsb/research/protein-labeling/Note that despite endogenous amber codons we see minimal background labeling of non-target proteins, either on the cell surface or intracellularly. -
Photochemical genetics
https://www2.mrc-lmb.cam.ac.uk/ccsb/research/photochemical-genetics/Comparison of the kinetics of ERK2–GFP nuclear accumulation following MEK photoactivation or endogenous receptor stimulation provided some interesting insights. -
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/55
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/55The pyrrolysyl-tRNA synthetase tRNAsubCUA/subu00a0pair is orthogonal with respect to the endogenous synthetases and tRNAs in emE. -
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/43
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/43In the first step we created an orthogonal ribosome that is selectively directed to a new orthogonal message that is not read by endogenous ribosomes. ... endogenous ribosome, creating a pool of potentially orthogonal ribosome binding sites. -
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/52
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/52the kinetics of elementary steps and adaptive feedback in kinase signalling./figcaption/figurenp /pnpComparison of the kinetics of ERK2u2013GFP nuclear accumulation following MEK photoactivation or endogenous receptor stimulation provided some -
Genetic code expansion in model organisms
https://www2.mrc-lmb.cam.ac.uk/ccsb/research/genetic-code-expansion-in-animals/The pyrrolysyl-tRNA synthetase tRNA. CUA. pair is orthogonal with respect to the endogenous synthetases and tRNAs in E. -
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/49
https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-json/wp/v2/pages/49Note that despite endogenous amber codons we see minimal background labeling of non-target proteins, either on the cell surface or intracellularly./figcaption/figurenpWe have demonstrated the efficient site-specific incorporation -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/19376
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/19376of DNA within the same strand or between opposite strands of the double-stranded helix, as a result of exposure to exogenous chemicals, such as chemotherapeutic drugs and alcohol, or endogenous -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/22
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/22{"id":22,"date":"2012-06-25T09:57:32","date_gmt":"2012-06-25T09:57:32","guid":{"rendered":"http://www2.mrc-lmb.cam.ac.uk/wordpress/?page_id=22"},"modified":"2023-07-21T12:49:25","modified_gmt":"2023-07-21T11:49:25","slug":"books","status":"publish"," -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/17787
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/17787{"id":17787,"date":"2020-04-09T09:36:35","date_gmt":"2020-04-09T08:36:42","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?page_id=17787"},"modified":"2024-04-23T16:38:13","modified_gmt":"2024-04-23T15:38:13","slug":"lmb-fellows","status":"publish -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/16108
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/16108{"id":16108,"date":"2020-07-10T12:49:35","date_gmt":"2020-07-10T09:49:56","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?page_id=16108"},"modified":"2024-05-31T17:30:23","modified_gmt":"2024-05-31T16:30:23","slug":"blue-sky-collaboration","statu -
HIV uses capsid pores to import nucleotides and evade innate immunity …
https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Fhiv-uses-capsid-pores-import-nucleotides-evade-innate-immunity%2F&format=xmlmrc-lmb.cam.ac.uk/wordpress/wp-content/uploads/Leo-James-HIV-Nature-cover-28-July-16.jpg 700 700 HIV is a retrovirus, meaning it has to copy its RNA -
Discovering the origins of biological timekeeping - MRC Laboratory of …
https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Fdiscovering-the-origins-of-biological-timekeeping%2F&format=xmlexistence of an endogenous daily clock in humans is well known: it is what drives the 24-hour sleep/wake rhythm to match the daily cycle of night and.
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