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Nanoscopic insights into seeding mechanisms and toxicity of…
https://laser.ceb.cam.ac.uk/system/files/documents/pnas-pintosi-2016.pdf3D), demonstrates that endogenous AS is pref-erentially recruited to elongate exogenous seeds rather thanundergoing nucleation. ... We find that these aggregates have a reducedpropensity to seed endogenous AS relative to preformed ASseed fibrils. -
Super-resolution imaging of alpha-synuclein polymorphisms and their…
https://laser.ceb.cam.ac.uk/system/files/documents/integrbiol-nugent-2017.pdfMore-over, our results showed that exogenously added monomerleads to the induction of endogenous a-synuclein aggregationwithout the two moieties coming into close proximity. ... incubated for 24 h in a-synuclein-free medium and immunostained for -
and Gabriele S. Kaminski SchierleEva-Maria Mandelkow, Clemens F.…
https://laser.ceb.cam.ac.uk/system/files/documents/jbc-michel-2013.pdfCrucially, the two-color assayrevealed the presence of both K18-647 and endogenous Tau in. ... The image displays K18-647 fibrils (red) thatare intertwined with endogenous Tau (green). -
Nanoscale imaging of neurotoxic proteins Clemens F. Kaminski,…
https://laser.ceb.cam.ac.uk/system/files/documents/spie-kaminski-2014.pdfIntriguingly, the cell expunges fibrillar aggregates that contain both the ingested K18Tau, shown in red, and endogenous wild type tau, already present in the cell, shown in green. ... F-1. Figure 5: Uptake of monomeric Tau protein into neuron-like cells -
Heparin acts as a structural component of β-endorphin…
https://laser.ceb.cam.ac.uk/system/files/documents/chemcomm-nespovitaya-2016.pdfrelevance of heparin-promoted amyloid models. The endogenous opiate b-endorphin is an essential neuropeptideinvolved in the stress response of higher organisms.1 In thesecretory pathway, it is temporarily stored in -
NPH-16016SSR 10..10 ++
https://laser.ceb.cam.ac.uk/system/files/documents/pdf-neurophoton-kaminski-2016.pdfFig. 6 Uptake of monomeric tau protein into neuron-like cells causes its aggregation and subsequentrecruitment of endogenous tau, normally present in the cell. ... Intriguingly, the cell expels fibrillaraggregates that contain both the ingested K18Tau, -
BMC BiologyBMC Biology This Provisional PDF corresponds to the ...
https://laser.ceb.cam.ac.uk/system/files/documents/bmcbio-avezoz-2015.pdfWhen transfected into COS7 cells the fluorescent signal produced a typical ER pattern that colocalized with the endogenous ER chaperone BiP (Figure 2B). ... B) Immunoblot of endogenous PDI1A in the cells above, with tubulin serving as a loading control. -
A cancer-associated BRCA2 mutation reveals masked nuclear export…
https://laser.ceb.cam.ac.uk/system/files/documents/jeyasekharan-nsmb-2013.pdfRed indicates DNA, cyan indicates BRCA2 (detected by anti-Flag) and purple indicates endogenous DSS1 (detected by anti-DSS1). ... a r t i c l e s. This mutant localized to the cytoplasm when expressed in DT40 cells lacking endogenous RAD51 (Fig. -
C-terminal calcium binding of α-synuclein modulates synaptic vesicle …
https://laser.ceb.cam.ac.uk/system/files/documents/natcommLautenschlger2018.pdf3c). Next, we studied the behavior of endogenous alpha-synucleinin ventral midbrain (VM) neurons incubated with 100 µMdopamine. ... VM neurons treatedwith dopamine for 72 h were stained against endogenous alpha-synuclein and the synaptic vesicle protein -
OP-BRAI140256 1..7
https://laser.ceb.cam.ac.uk/system/files/documents/brain-frischi-2014.pdfendogenous amyloid-b deposition in the hippocampus beginsto appear at 8–10 months of age. -
Supercontinuum radiation in fluorescence microscopy and biomedical…
https://laser.ceb.cam.ac.uk/system/files/documents/josab362A139.pdf4) toinvestigate health states of tissues. The autofluorescence lifetimecharacteristics can be studied by simply exciting endogenous flu-orophores without labels [5,61] at desired wavelengths using aSC source. -
TriPer, an optical probe tuned to the endoplasmic reticulum tracks…
https://laser.ceb.cam.ac.uk/system/files/documents/bmc-biol-melo-2017.pdfFig. 1 Glutathione depletion sensitizes pancreatic β-cells to endogenous H2O2. a Absorbance at 540 nm (an indicator of cell mass) by cultures ofa β-cell line (RINm5F) or mouse embryonic -
Science Journals — AAAS
https://laser.ceb.cam.ac.uk/system/files/documents/science-gallardo-2016.pdfMoreover, wefound that the peptide is able to enter cells andreach the cytoplasmic compartment and specificallyinduce the aggregation of endogenous VEGFR2,inhibiting its function in human umbilical veinendothelial cells (HUVECs) in -
190225_manuscript file_figures (1)
https://laser.ceb.cam.ac.uk/system/files/documents/intra2010mitochondrial-protein-degradation.pdfaggregation of normally folded endogenous alpha‐synuclein (Luk et al, 2009; Pinotsi et al, 66. 2016). Since the seeding of alpha‐synuclein is understood as a driving force for disease 67.
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