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doi:10.1016/j.molcel.2004.08.030
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf20 Sep 2004: Cells separatedsister chromatids with identical kinetics to those thatof the GAL1-10 promoter in cells with an endogenous. ... Nevertheless, noncleavableScc1-HA3 from the endogenous SCC1 promoter. The. sole copy of Cdc20 in all three strains was -
The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf28 Nov 2008: G) Epifluorescence micrographs of a HeLa cell incubated with CTxB for 5 min before fixation and staining for endogenous GRAF1. ... B, Confocal micrograph of an NIH 3T3 cell stained for endogenous GRAF1 distribution. -
TBS may template.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/mcmahon.pdf3 May 2002: This might be caused bycompetition for clathrin binding between the injectedpeptide and the endogenous protein, therebypreventing endogenous AP180 and AP2 frompromoting clathrin assembly at the plasmamembrane. -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. -
554 Research Paper Inhibition of receptor-mediated endocytosis by the …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf21 Mar 2003: The most likely explanation for this is that theinteraction of dynamin with endogenous amphiphysin wasdisrupted in these cells. ... J Cell Biol 1993, 127:725-735. 18. Tuma PL, Stachniak MC, Collins CA: Activation of dynamin GTPaseby acidic phospholipids -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: Catalytically inactive UBE2O(C1040S) alsorecovered endogenous and exogenous RPL24(but no detectable ubiquitinated forms), whereasUBE2O(DCR2) interacted poorly with RPL24 (fig.S10D). ... The heterogeneity of ubiquitin bands in the samples with cytosol is -
Features critical for membrane binding revealed by DivIVA crystal…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf20 May 2010: tionally complements an endogenous divIVA replacement. This was also seen when sporulation of these strains was. ... endogenous DivIVA through dimerization or oligomerization. When localization of wild-type DivIVA-GFP was analysed in. -
Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf4 Jan 2006: various endophilins and controls. a. b. c. Transformation of JC201 bacteria with endophilin does not lead to an acceleration of growth kinetics, unlike transformation of the endogenous LPAAT. -
The structural basis of novel endosome anchoringactivity of KIF16B ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17641687.pdf23 Jul 2007: performed dual immunofluorescence staining of expressed. KIF16B-PX and endogenous EEA1 using fixed HEK293 cells. ... In agreement with A431 cell localization, KIF16B-PX was. colocalized with endogenous EEA1 (Figure 6A). -
doi:10.1016/j.devcel.2004.09.003
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15469844.pdf1 Nov 2004: InhVps37A and hVps37B (Bache et al., 2004; Stuchell et al.,addition, they play a critical role in retrovirus budding.2004), while the human ESCRT-II subunits correspondStructural understanding of ESCRT interaction ... The CCP4 suite: Morita, E., and -
tra_578 1..18
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Burtey%20IVF%20Traffic%2007.pdf15 May 2007: colocalizing with endogenous AP-2 complexes (Figure 2. G–J). In contrast, the IVA mutation resulted in complete. ... plasma membrane (Figure 5A), which colocalized with both. clathrin–DsRed and endogenous AP-2 complexes (Figure 5. -
The ER membrane protein complex promotes biogenesis of sterol-related …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/JCS_2019.pdf18 Jan 2019: SQS insertion by EMC enables sterol biosynthesisTo determine the mechanism responsible for downregulating SQSin EMC-knockout cells, we monitored endogenous SQS synthesisand turnover by performing 35S-Met/Cys pulse-chase assays. ... A) 35S-Met/Cys -
Filament structure and subcellular organization of the bacterial…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Liu_PNAS_crescentin_2024.pdf28 Apr 2024: This yielded protein levels of CreS similar to endogenous levels in wt strain CB15N (SI Appendix, Fig. ... Subcellular Organization of CreS Filaments. The ultrastructure of endogenous CreS assemblies at normal levels in C. -
pnas201206839 17424..17429
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/23045692.pdf21 Nov 2012: Depletion of endogenous MITD1with two independent small interfering RNAs (siRNAs) resultedin phenotypes consistent with cytokinesis failure, as indicated bya six- to eightfold increase of multinucleated cells compared withcontrol siRNA-transfected -
A Ubiquitin Ligase-Associated Chaperone Holdase Maintains…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/72_Wang_Q_Mol_Cell_2011.pdf23 May 2012: IB, immunoblot-. ting. (B and C) Endogenous interactions between the. Bag6 and the gp78-p97 complexes. ... Trc35 Maintains a Cytosolic Pool of Bag6(A) Trc35 expression alters the subcellular localization of endogenous Bag6. -
cm6307.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TAXOL.C&B.pdf18 Feb 1999: Such molecules mightmimic an endogenous cellular factor, presumably a lesspotent one, that helps to stabilise microtubules in normalcytoplasm; the natural reagent might be a small molecule orpart of a microtubule-associated -
Sensory Neurons Arouse C. elegans Locomotion via Both Glutamate and…
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-4.pdf30 Jun 2015: Under these conditions, we only observed endogenous acetylcholine EPSCs. To recordGABAergic postsynaptic currents, the holding potential was 0 mV, at which we only observemIPSCs. ... Supporting InformationS1 Fig. Stimulus-evoked EPSCs and endogenous -
No Job Name
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/vandenent2008ftsq.pdf4 Mar 2008: As shown in Fig. 5, WTFtsQ expressed together with sYFP1-FtsL in LMC531cells (that contain the endogenous temperature-sensitivemutation E125K) target FtsL to midcell at both 28C and42C (Fig. ... The resulting strains wereconfirmed by PCR analysis, -
Discovering the origins of biological timekeeping - MRC Laboratory of …
https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Fdiscovering-the-origins-of-biological-timekeeping%2F&format=xmlexistence of an endogenous daily clock in humans is well known: it is what drives the 24-hour sleep/wake rhythm to match the daily cycle of night and. -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: coli ETEC H10407 was investigated. The protein at endogenous expression levels is easilydetected and this signal disappears in a leoA KO strain, demonstrating specificity. -
doi:10.1016/j.ceb.2003.11.005
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cocb%202003.pdf27 Nov 2003: Immunofluorescence microscopy of endogenous Mbl in fixed cells gave. similar images. -
Molecular Biology of the CellVol. 18, 3681–3691, September 2007 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/48_Rutkowski_DT_MBC_2007.pdf15 Aug 2007: ER stress did not alterthe electrophoretic migration or protease protection ofp58IPK (Figure 3A), suggesting that there is no substantialrelocalization of endogenous p58IPK during ER stress. ... These results demon-strate a specific interaction between ER -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahon01020.pdf3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. -
Functional Depletion of Mahogunin by Cytosolically Exposed Prion…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/59_Chakrabarti_O_Cell_2009.pdf13 Jun 2009: PrP would sufficiently influence endogenous Mgrn localization. to at least partially phenocopy a Mgrn depletion. ... Because endogenous Mgrn appears to normally. be associated tightly with membranes, we could not use selec-. -
Interaction of tau protein with the dynactincomplex Enrico…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBOJ2007-Magnani-Fan.pdf15 Oct 2007: A) Endogenous p150 co-immunopre-cipitates with tau in differentiated SH-SY5Y cells. Immunoprecipitation was performed with anti-tau antibody BR133 and immunoblots withp150 antibody. ... transgenic mice (data not shown) has been hampered by a. likely -
Protein targeting and degradation are coupled for elimination of…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/73_Hessa_T_Nature_2011.pdf14 Jul 2011: endogenous ubiqui-tin was more than 90% competed out, resulting in few or no untagged ubiquiti-nated products. ... The absence of endogenous charged tRNAs and haemoglobinincreased photocrosslinker incorporation and photolysis, respectively. -
Cryo‐EM structure of the full‐length Lon protease from Thermus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf14 Nov 2021: the ATP-dependent Lon protease- lessons from. endogenous protein and synthetic peptide substrates. -
A Conserved Archaeal Pathway for TailAnchored Membrane Protein…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/70_Sherrill_J_Traffic_2011.pdf11 Nov 2011: We also examined whether archaeal TRC40 could func-tionally replace the endogenous mammalian TRC40 atphysiological concentrations. ... absence of endogenous mam-malian TRC40 using a phenyl- and Diethylaminoethyl (DEAE)-Sepharose-depleted RRL (PD-RRL) -
ppat.1000479 1..19
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/60_Ashok_A_PLoS_Path_2009.pdf20 Jun 2009: completely unrelated human cell line that expresses very low (but. detectable) levels of endogenous PrP. ... presence or absence of endogenous PrP in co-expression. experiments (e.g., as in Fig. -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/9259
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/9259HIV is a retrovirus, meaning it has to copy its RNA genome into DNA in order to infect cells. -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: coli cells, we constructed C-terminal fusions of the fluorescentprotein YPet to wild-type FtsK and truncated derivatives of it ex-pressed from the endogenous wild-type chromosomal locus. -
MinCD cell division proteins form alternating copolymeric cytomotive…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ncomms6341.pdf6 Dec 2014: Unfortunately, it is currently not possible to reliablyimage at endogenous levels of MinC with SIM. -
doi:10.1016/j.cub.2004.09.077
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Snx1PeteCullen2004/Snx1PeteCullen2004.pdf23 Nov 2004: Endogenous SNX1 was enriched on tubularmembranes in close vicinity to endosomal vacuoles (Fig-ure 3). ... These data establish a role for endogenous SNX1in the retrieval of the CI-MPR from early endosomes tothe TGN. -
doi:10.1016/j.cub.2004.07.058
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15296756.pdf6 Oct 2004: The challenge now is to establish the molecularhave similar phenotypes: they retain a similar shape tolinks between endogenous ARAP3’s control of Rho andcontrol cells, but on PDGF stimulation their lamellipodiaArf -
25 Structural studies of phosphoinositide 3-kinase-dependent traffi c …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17233579_proof.pdf14 Jun 2007: How do various ESCRT components link to other processes such as pH regulation of gene expression [51] or retrovirus budding [52]? -
FtsA forms actin-like protofilaments
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276a.pdf27 Mar 2012: away from the nucleotide-binding site of FtsA (Figure 2A,. right), which contains an endogenous ATP molecule at. ... appearance that is straightforward to distinguish and. quantify. We then complemented the endogenous ts-FtsA. -
The Rockefeller University Press, 0021-9525/97/02/567/15 $2.00The…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/8_Lingappa_JE_JCB_1997.pdf3 Feb 1997: . The Rockefeller University Press, 0021-9525/97/02/567/15 $2.00The Journal of Cell Biology, Volume 136, Number 3, February 10, 1997 567–581 567. A Multistep, ATP-dependent Pathway for Assembly ofHuman Immunodeficiency Virus Capsids in a -
ANRV378-BI78-31.tex
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DohertyMcMahonAnnRevBiochem09.pdf27 Apr 2009: However, the endocytosisof many other types of endogenous and exoge-nous cargoes is relatively unaffected by inhibi-tion of this pathway. ... Relatively few endogenous, noncargo pro-teins associated with clathrin-independent. endocytic pathways have been -
Evolving nature of the AP2 a-appendage hubduring clathrin-coated…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/AP2Hub_EMBO2004/AP2Praefcke2004.pdf23 Nov 2004: The puncta. begin to appear at temperatures below 181C and colocalisewith endogenous AP2. ... 6C. 6C. 37C. Endogenous AP2Overexpressed epsins Transferrin uptakeA. B. C (d) Coated vesicle assemblyClathrin. -
Effects of Epsin R overexpression
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/epsinrD34GR67L.html25 Jun 2007: Myc-EpsinR D34G R67L with endogenous AP1 and internalised transferrin in COS cells. -
Discovering the origins of biological timekeeping - MRC Laboratory of …
https://www2.mrc-lmb.cam.ac.uk/discovering-the-origins-of-biological-timekeeping/20 Apr 2015: Published on. 20 April, 2015. The existence of an endogenous daily clock in humans is well known: it is what drives the 24-hour sleep/wake rhythm to match the daily
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Kate McDole - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/group-leaders/h-to-m/kate-mcdole/21 Jul 2023: Generation and live imaging of an endogenous Cdx2 reporter mouse line..
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Biochem. J. (2011) 440, 185–193 (Printed in Great Britain) ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/ChernomordikBJ2011.pdf15 Nov 2011: To evaluatePtdIns(4,5)P2 content in the plasma membrane and to competefor PtdIns(4,5)P2 binding with endogenous PtdIns(4,5)P2-bindingproteins, we expressed PLCδ1PH (PH domain of ... Lowering the concentrations of PtdIns(4,5)P2available for interactions -
doi:10.1016/j.cell.2007.01.036
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/46_Stefanovic_S_Cell_2007.pdf13 Apr 2015: Totest this, we analyzed the interactions between Sec61band endogenous TRC40 versus exogenously added (andepitope-tagged) TRC40-DN. ... with release from endogenous TRC40. 1156 Cell 128, 1147–1159, March 23, 2007 ª2007 Elsevier Inc. -
The structure of human thyroglobulin
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf31 Jan 2020: rTG appears to be indistinguishable by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and cryo-electron microscopy (cryo-EM) from endogenous TG (eTG) puri-fied from thyroid glands of patients -
Welcome to Adobe GoLive 6
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/Large_compartment.html2 Nov 2002: Endogenous EpsinR. Overexpressed. Myc-Epsin1. High levels of overexpression lead to grossly enlarged perinuclear compartments These compartments colabel with M6P receptors and TGN46 but do not co-label with endogenous GM130 -
EpsinR Home page: a ubiquitous membrane bending molecule that…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/epsinRhome.html26 Sep 2011: For a detailed study of endogenous epsinR and overexpression of wild-type and mutants in cells see immunocytochemistry. -
Cryptochrome proteins are integral to maintain time within the…
https://www2.mrc-lmb.cam.ac.uk/cryptochrome-proteins-are-integral-to-maintain-time-within-the-brains-master-clock/20 Jan 2022: Published on. 20 January, 2022. A schematic showing the effect of CRY1 on the relationships between localisation and intracellular mobility of endogenous fluorescently tagged PER2 as well as circadian rhythmicity of ... Real-time imaging of endogenous
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Articleshttps://doi.org/10.1038/s41594-019-0196-z 1MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%202019%20NSMB.pdf4 Mar 2019: Articleshttps://doi.org/10.1038/s41594-019-0196-z. 1MRC Laboratory of Molecular Biology, Cambridge, UK. 2Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK. 3Bioanalytics, Institute of Biotechnology, Technische -
elifesciences.org van den Ent et al. eLife 2014;3:e02634. DOI: ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mrebdouble2014.pdf6 Jun 2014: elifesciences.org. van den Ent et al. eLife 2014;3:e02634. DOI: 10.7554/eLife.02634 1 of 22. Bacterial actin MreB forms antiparallel double filamentsFusinita van den Ent1†, Thierry Izoré1†, Tanmay AM Bharat1, Christopher M Johnson2, Jan Löwe1.
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