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27 Jan 2018: This system contains endogenous mRNAs (primarily encoding the 14 kD globins and a prominent 70 kD protein) that contribute to background, but has the advantage of retaining native cytosolic conditions, most ... aureus is used to digest endogenous mRNA.

14 Nov 2014: Scc1’s endogenous M1 has been deleted, an N-terminal myc9 epitope was fused in frame and endogenous M30 was changed to I30. ... Endogenous Scc1M102, Smc3M139 and M186 generate a 30 kDa Scc1 and a 5 kDa Smc3 fragment.

21 Mar 2003: Mol Biol Cell 1998, 9:195a. 22. Tuma PL, Stachniak MC, Collins CA: Activation of dynamin GTPaseby acidic phospholipids and endogenous rat brain vesicles. ... such adynaminPH construct into COS cells will be even betterat sequestering endogenous dynamin

18 Sep 2008: Ifn-PrP. Note the lack of changes to endogenous PrP in cells expressing. ... Thus, in both cases, Ifn-PrP is not being overexpressed relative. to endogenous PrP.

4 Jan 2006: A JC201 strain ofEscherichia coli lacking endogenous LPAAT activity grows at 30 8Cbut not at 42 8C (ref. ... coli that has no endogenous LPAATactivity (JC201 strain) cannot growat 42 8C (sector 1).

10 Nov 2000: 1B), despitethe fact that endogenous Vg1 was expressed only in the late-stageoocytes (Fig. ... 1C). This result raisesthe possibility that the endogenous, localized Vg1 message is inan environment that is free from repression.

3 May 2002: This might be caused bycompetition for clathrin binding between the injectedpeptide and the endogenous protein, therebypreventing endogenous AP180 and AP2 frompromoting clathrin assembly at the plasmamembrane.

19 Mar 2013: Amph12 RNAi) depletion on the recruitment of endogenous dynamin 2 (dynaminen, green and white arrows) and clathrin light chain A (clathrinen, red). ... Bar, 10µm (B) Effect of SNX9, amphiphysin and endophilin overexpression on endogenous dynamin 2

20 Nov 2003: S6. Colocalization of wild type, mutant, and endogenous arfaptin with TGN46 Table S1.

2 Jun 2004: 1B).BAPGRA4 was localized to dense granules as evidenced bycolocalization with the endogenous dense granule proteinGRA3 (32). ... Both endogenous GRA4 andBAPGRA4 were efficiently translocated across the ER mem-brane (Fig.

20 Sep 2004: Cells separatedsister chromatids with identical kinetics to those thatof the GAL1-10 promoter in cells with an endogenous. ... Nevertheless, noncleavableScc1-HA3 from the endogenous SCC1 promoter. The. sole copy of Cdc20 in all three strains was

28 Nov 2008: G) Epifluorescence micrographs of a HeLa cell incubated with CTxB for 5 min before fixation and staining for endogenous GRAF1. ... B, Confocal micrograph of an NIH 3T3 cell stained for endogenous GRAF1 distribution.

3 May 2002: This might be caused bycompetition for clathrin binding between the injectedpeptide and the endogenous protein, therebypreventing endogenous AP180 and AP2 frompromoting clathrin assembly at the plasmamembrane.

3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong.

21 Mar 2003: The most likely explanation for this is that theinteraction of dynamin with endogenous amphiphysin wasdisrupted in these cells. ... J Cell Biol 1993, 127:725-735. 18. Tuma PL, Stachniak MC, Collins CA: Activation of dynamin GTPaseby acidic phospholipids

4 Aug 2017: Catalytically inactive UBE2O(C1040S) alsorecovered endogenous and exogenous RPL24(but no detectable ubiquitinated forms), whereasUBE2O(DCR2) interacted poorly with RPL24 (fig.S10D). ... The heterogeneity of ubiquitin bands in the samples with cytosol is

20 May 2010: tionally complements an endogenous divIVA replacement. This was also seen when sporulation of these strains was. ... endogenous DivIVA through dimerization or oligomerization. When localization of wild-type DivIVA-GFP was analysed in.

4 Jan 2006: various endophilins and controls. a. b. c. Transformation of JC201 bacteria with endophilin does not lead to an acceleration of growth kinetics, unlike transformation of the endogenous LPAAT.

23 Jul 2007: performed dual immunofluorescence staining of expressed. KIF16B-PX and endogenous EEA1 using fixed HEK293 cells. ... In agreement with A431 cell localization, KIF16B-PX was. colocalized with endogenous EEA1 (Figure 6A).

1 Nov 2004: InhVps37A and hVps37B (Bache et al., 2004; Stuchell et al.,addition, they play a critical role in retrovirus budding.2004), while the human ESCRT-II subunits correspondStructural understanding of ESCRT interaction ... The CCP4 suite: Morita, E., and

15 May 2007: colocalizing with endogenous AP-2 complexes (Figure 2. G–J). In contrast, the IVA mutation resulted in complete. ... plasma membrane (Figure 5A), which colocalized with both. clathrin–DsRed and endogenous AP-2 complexes (Figure 5.

18 Jan 2019: SQS insertion by EMC enables sterol biosynthesisTo determine the mechanism responsible for downregulating SQSin EMC-knockout cells, we monitored endogenous SQS synthesisand turnover by performing 35S-Met/Cys pulse-chase assays. ... A) 35S-Met/Cys

28 Apr 2024: This yielded protein levels of CreS similar to endogenous levels in wt strain CB15N (SI Appendix, Fig. ... Subcellular Organization of CreS Filaments. The ultrastructure of endogenous CreS assemblies at normal levels in C.

21 Nov 2012: Depletion of endogenous MITD1with two independent small interfering RNAs (siRNAs) resultedin phenotypes consistent with cytokinesis failure, as indicated bya six- to eightfold increase of multinucleated cells compared withcontrol siRNA-transfected

23 May 2012: IB, immunoblot-. ting. (B and C) Endogenous interactions between the. Bag6 and the gp78-p97 complexes. ... Trc35 Maintains a Cytosolic Pool of Bag6(A) Trc35 expression alters the subcellular localization of endogenous Bag6.

18 Feb 1999: Such molecules mightmimic an endogenous cellular factor, presumably a lesspotent one, that helps to stabilise microtubules in normalcytoplasm; the natural reagent might be a small molecule orpart of a microtubule-associated

30 Jun 2015: Under these conditions, we only observed endogenous acetylcholine EPSCs. To recordGABAergic postsynaptic currents, the holding potential was 0 mV, at which we only observemIPSCs. ... Supporting InformationS1 Fig. Stimulus-evoked EPSCs and endogenous

4 Mar 2008: As shown in Fig. 5, WTFtsQ expressed together with sYFP1-FtsL in LMC531cells (that contain the endogenous temperature-sensitivemutation E125K) target FtsL to midcell at both 28C and42C (Fig. ... The resulting strains wereconfirmed by PCR analysis,

existence of an endogenous daily clock in humans is well known: it is what drives the 24-hour sleep/wake rhythm to match the daily cycle of night and.

3 Sep 2014: coli ETEC H10407 was investigated. The protein at endogenous expression levels is easilydetected and this signal disappears in a leoA KO strain, demonstrating specificity.

27 Nov 2003: Immunofluorescence microscopy of endogenous Mbl in fixed cells gave. similar images.

15 Aug 2007: ER stress did not alterthe electrophoretic migration or protease protection ofp58IPK (Figure 3A), suggesting that there is no substantialrelocalization of endogenous p58IPK during ER stress. ... These results demon-strate a specific interaction between ER

3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong.

13 Jun 2009: PrP would sufficiently influence endogenous Mgrn localization. to at least partially phenocopy a Mgrn depletion. ... Because endogenous Mgrn appears to normally. be associated tightly with membranes, we could not use selec-.

15 Oct 2007: A) Endogenous p150 co-immunopre-cipitates with tau in differentiated SH-SY5Y cells. Immunoprecipitation was performed with anti-tau antibody BR133 and immunoblots withp150 antibody. ... transgenic mice (data not shown) has been hampered by a. likely

14 Jul 2011: endogenous ubiqui-tin was more than 90% competed out, resulting in few or no untagged ubiquiti-nated products. ... The absence of endogenous charged tRNAs and haemoglobinincreased photocrosslinker incorporation and photolysis, respectively.

14 Nov 2021: the ATP-dependent Lon protease- lessons from. endogenous protein and synthetic peptide substrates.

11 Nov 2011: We also examined whether archaeal TRC40 could func-tionally replace the endogenous mammalian TRC40 atphysiological concentrations. ... absence of endogenous mam-malian TRC40 using a phenyl- and Diethylaminoethyl (DEAE)-Sepharose-depleted RRL (PD-RRL)

20 Jun 2009: completely unrelated human cell line that expresses very low (but. detectable) levels of endogenous PrP. ... presence or absence of endogenous PrP in co-expression. experiments (e.g., as in Fig.

23 Dec 2013: coli cells, we constructed C-terminal fusions of the fluorescentprotein YPet to wild-type FtsK and truncated derivatives of it ex-pressed from the endogenous wild-type chromosomal locus.

HIV is a retrovirus, meaning it has to copy its RNA genome into DNA in order to infect cells.

6 Dec 2014: Unfortunately, it is currently not possible to reliablyimage at endogenous levels of MinC with SIM.

6 Oct 2004: The challenge now is to establish the molecularhave similar phenotypes: they retain a similar shape tolinks between endogenous ARAP3’s control of Rho andcontrol cells, but on PDGF stimulation their lamellipodiaArf

14 Jun 2007: How do various ESCRT components link to other processes such as pH regulation of gene expression [51] or retrovirus budding [52]?

23 Nov 2004: Endogenous SNX1 was enriched on tubularmembranes in close vicinity to endosomal vacuoles (Fig-ure 3). ... These data establish a role for endogenous SNX1in the retrieval of the CI-MPR from early endosomes tothe TGN.

27 Mar 2012: away from the nucleotide-binding site of FtsA (Figure 2A,. right), which contains an endogenous ATP molecule at. ... appearance that is straightforward to distinguish and. quantify. We then complemented the endogenous ts-FtsA.

27 Apr 2009: However, the endocytosisof many other types of endogenous and exoge-nous cargoes is relatively unaffected by inhibi-tion of this pathway. ... Relatively few endogenous, noncargo pro-teins associated with clathrin-independent. endocytic pathways have been

3 Feb 1997: . The Rockefeller University Press, 0021-9525/97/02/567/15 $2.00The Journal of Cell Biology, Volume 136, Number 3, February 10, 1997 567–581 567. A Multistep, ATP-dependent Pathway for Assembly ofHuman Immunodeficiency Virus Capsids in a

23 Nov 2004: The puncta. begin to appear at temperatures below 181C and colocalisewith endogenous AP2. ... 6C. 6C. 37C. Endogenous AP2Overexpressed epsins Transferrin uptakeA. B. C (d) Coated vesicle assemblyClathrin.

25 Jun 2007: Myc-EpsinR D34G R67L with endogenous AP1 and internalised transferrin in COS cells.