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26 Sep 2011: For a detailed study of endogenous epsinR and overexpression of wild-type and mutants in cells see immunocytochemistry.

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{"id":10846,"date":"2017-08-17T10:46:43","date_gmt":"2017-08-17T09:46:43","guid":{"rendered":"http://www2.mrc-lmb.cam.ac.uk/?p=10846"},"modified":"2021-07-19T13:48:36","modified_gmt":"2021-07-19T12:48:36","slug":"folate-and-formaldehyde-from-vitamin-

{"id":15741,"date":"2019-10-31T09:28:41","date_gmt":"2019-10-31T09:28:41","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?p=15741"},"modified":"2022-03-23T13:05:14","modified_gmt":"2022-03-23T13:05:14","slug":"decade-long-collaboration-results-in

Note that despite endogenous amber codons we see minimal background labeling of non-target proteins, either on the cell surface or intracellularly.

19 Jan 2017: endogenous proteins with recombinant variantsrevealed the functional roles for all of the factorsand interactions. ... C) Immunoblots (IB) for triage components of control or BAG6 complex-depleted RRL replenished with recombinant BAG6 sub-complexes used

20 Apr 2015: Published on. 20 April, 2015. The existence of an endogenous daily clock in humans is well known: it is what drives the 24-hour sleep/wake rhythm to match the daily

In the first step we created an orthogonal ribosome that is selectively directed to a new orthogonal message that is not read by endogenous ribosomes. ... We first selected, on 5FU, for sequences upstream of the cat-upp fusion that do not allow the gene

2 Nov 2002: Endogenous EpsinR. Overexpressed. Myc-Epsin1. High levels of overexpression lead to grossly enlarged perinuclear compartments These compartments colabel with M6P receptors and TGN46 but do not co-label with endogenous GM130

Comparison of the kinetics of ERK2–GFP nuclear accumulation following MEK photoactivation or endogenous receptor stimulation provided some interesting insights.

16 May 2018: This method is the first widely applicable protein degradation method that acts directly on the level of the endogenous protein.

We develop biochemical methodsnto obtain protein-nucleic acid complexes involved in telomere maintenancenpathways both from endogenous sources and by overexpression methods.

25 Oct 2006: Fig. S6. Colocalization of wild type, mutant, and endogenous full-length arfaptin2 with TGN46.

The pyrrolysyl-tRNA synthetase tRNAsubCUA/subu00a0pair is orthogonal with respect to the endogenous synthetases and tRNAs in emE.

12 Mar 2012: The research team included collaborators from the MRC Centre for Medical Molecular Virology and the Retrovirus Research Laboratory, MRC University of Glasgow Centre for Virus Research.

In the first step we created an orthogonal ribosome that is selectively directed to a new orthogonal message that is not read by endogenous ribosomes. ... endogenous ribosome, creating a pool of potentially orthogonal ribosome binding sites.

21 Jul 2023: Paperback, 135pp. Includes: Technology Transfer in Britain: The case of Monoclonal Antibodies, Self and Non-self: A history of Autoimmunity, Endogenous Opiates, The Committee on Safety of Drugs.

Note that despite endogenous amber codons we see minimal background labeling of non-target proteins, either on the cell surface or intracellularly./figcaption/figurenpWe have demonstrated the efficient site-specific incorporation

The pyrrolysyl-tRNA synthetase tRNA. CUA. pair is orthogonal with respect to the endogenous synthetases and tRNAs in E.

5 May 2022: Enzyme-catalyzed proximity labeling (PL) has emerged as a powerful and generalizable method to map the locations and interactions of endogenous proteins in the context of living cells, applicable to even

25 Sep 2015: This was because endogenous formaldehyde destroyed the blood stem cells residing in the bone marrow. ... In these patients reducing or counteracting endogenous formaldehyde production may be of therapeutic value.

10 Jul 2019: KAP1 is the primary regulator of these retrovirus insertions. Guido Stoll, a PhD student in Yorgo’s group, used X-ray crystallography to determine the structure of the part of KAP1

of DNA within the same strand or between opposite strands of the double-stranded helix, as a result of exposure to exogenous chemicals, such as chemotherapeutic drugs and alcohol, or endogenous

18 May 2022: Replisomes facing exogenous and endogenous stress, or replisome components that have become mutated, have also been identified as a characteristic of cancerous cells.

12 Jul 2024: https://www2.mrc-lmb.cam.ac.uk/news-events/public-engagement/cryo-electron-microscopy/ One of the world's leading research institutes, our scientists are working to advance understanding of biological processes at the molecular level - providing the

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25 Jun 2007: Endogenous epsinR in COS cells shows a TGN localisation and also puncta in the cytoplasm.

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8 Aug 2014: microscopy (Fig 4A). (B) Cells with endogenous Scc1 or Scc3 (K20783) C-terminal. ... at the indicated time points and compared to levels made by endogenous tagged.

30 Mar 2012: To analyze endocytic dynamics in mammalian cells in which endogenous protein stoichiometry is preserved, we previously targeted zinc finger nucleases (ZFNs) to the clathrin light chain A and dynamin-2 genomic

22 Jul 2007: cells infected with retrovirus only. Expression of intrabody. causes a morphological change in HT-1080 with reorganised. ... mice. All mice subcutaneously injected with EJ cells infected. with retrovirus only or retrovirus expressing the mutant.

17 Aug 2017: They further showed that when the protection against this molecule is removed in mice then the levels of endogenous formaldehyde rise leading to overwhelming DNA damage and rapid death.

9 Jan 2003: Figure 6. Subcellular localization of epsinR. As a convention throughout the figure, myc-epsinR and mutants are labeled green and the endogenous proteins are labeled red. ... 3), and homologues are found in bothC. elegans and Drosophila. Thus, we used

17 Jan 2006: 5A,B). Furthermore,there was a clear enrichment of endogenous SNX2 on SNX1. ... Cells were then fixed and stained againstendogenous SNX1, endogenous SNX2 and endogenous mVps26.

28 Nov 2006: 1d). Under our experimental conditions, endogenous levelsof MKK3 protein could not be detected by using the anti-MKK3antibody. ... This effect of expressed YopJ on endogenous MEK1/2was seen as early as 6 h after transfection of cells (data notshown).

19 Jun 2006: J Cell Biol 129: 1509–1522. Morita E, Sundquist WI (2004) Retrovirus budding. ... Keywords: prions; retrovirus budding; exosome; endosomal transport;lysosome; multivesicular bodySubmitted 24 May 2006; accepted 31 May 2006.

20 Oct 2015: elegans neurons using cell-specificpromoters. Since connexins should not. interact with endogenous innexins fromother neighboring neurons, we expected anew gap junction to form exclusivelybetween connexin-expressing neurons Aand B (Fig. ... elegans

8 Nov 2021: Yara Sanchez-Corrales, a postdoc in Katja’s lab, tagged endogenous proteins to label cell shapes in Drosophila embryos.

22 Mar 2005: Bar 10 m. endogenous MPR300 and TGN46 were relocalized to these conditions was not addressed directly. ... In agreement with these observations, we foundthat the endogenous EGF receptor was not relocalizedmembranes.

31 Oct 2019: to exogenous chemicals, such as chemotherapeutic drugs and alcohol, or endogenous by-products of normal cellular metabolism.

27 Jan 2018: This system contains endogenous mRNAs (primarily encoding the 14 kD globins and a prominent 70 kD protein) that contribute to background, but has the advantage of retaining native cytosolic conditions, most ... aureus is used to digest endogenous mRNA.

14 Nov 2014: Scc1’s endogenous M1 has been deleted, an N-terminal myc9 epitope was fused in frame and endogenous M30 was changed to I30. ... Endogenous Scc1M102, Smc3M139 and M186 generate a 30 kDa Scc1 and a 5 kDa Smc3 fragment.

26 Feb 2019: c, Growth of strains containing point mutations at the elbow in the endogenous mukB gene.

1 Dec 2010: together with endogenous Smc3. Western blotting revealed. that immunoprecipitates of Smc3-HA3 or Smc3AAA-HA3. ... As expected, similar amounts of Smc1-myc9 and endogenous. Smc1 associate with Smc3-HA3.

21 Mar 2003: Mol Biol Cell 1998, 9:195a. 22. Tuma PL, Stachniak MC, Collins CA: Activation of dynamin GTPaseby acidic phospholipids and endogenous rat brain vesicles. ... such adynaminPH construct into COS cells will be even betterat sequestering endogenous dynamin

3 May 2002: This might be caused bycompetition for clathrin binding between the injectedpeptide and the endogenous protein, therebypreventing endogenous AP180 and AP2 frompromoting clathrin assembly at the plasmamembrane.

3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong.

20 Nov 2003: S6. Colocalization of wild type, mutant, and endogenous arfaptin with TGN46 Table S1.

20 May 2010: tionally complements an endogenous divIVA replacement. This was also seen when sporulation of these strains was. ... endogenous DivIVA through dimerization or oligomerization. When localization of wild-type DivIVA-GFP was analysed in.