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4 Oct 2010: remove endogenous AcCoA) and used in acetyltransferaseassays.Size-exclusion Chromatography of HeLa Cytosol—The re-.

7 Oct 2005: he same for both Crt and Prl-Crt and were comparableo the levels of endogenous Crt (Figure 5A). ... A) Crt/ cells stably expressing either Crt-GFP or Prl-Crt-GFPwere analyzed (in duplicate) by immunoblotting with anti-Crt andanti-Sec61β (an endogenous

3 Sep 2008: Similar endogenous fila-. ments (presumably of ESCRT-III components) were observed. when an ATP-locked mutant of Vps4 (VPS4BE235Q) was overex-.

11 Nov 2011: This indicated that BirA has no detectable targetsendogenous to mammalian cells, and that the BioTag is notmodified by endogenous enzymes. ... Note that lowlevels of endogenous PrP are visualized at 35kDa in the SS-PrP samples probed with-PrP.

9 Dec 1999: Trans-genic (Tg) mice expressing each of these mutant PrPs in the FVB/Pmp0/0 background (null for the endogenous PrP gene) weregenerated and characterized (see Table 1 and Fig.

30 Apr 2010: Fraction 5 of the flow through stained endogenous Doc2a and -b with no remaining signal of each protein in single and double-knockout mice.

9 Sep 2015: Upon induction with tetracycline, both wild-type and mutant Sec61 transgenes (untagged) were expressed at similar levels as the endogenous protein (Figure 6—figure supplement 1C). ... Because we could not easily distinguish endogenous Sec61 from the

11 Aug 2015: Because stable association of NEMF with 60S requires a peptidyl-tRNA (Shao et al., 2015), these complexes are likely to contain endogenous nascent chains. ... We examined this idea by analysis of endogenous RNCs on ER microsomes isolated from native

21 Sep 2006: We therefore stably expressed Crt-GFP in crt/ fibroblasts at alevel corresponding to endogenous Crt levels of matched crt/ cells(Fig.

31 Aug 2006: Role of the AP2 b-Appendage Hubin Recruiting Partners for Clathrin-CoatedVesicle AssemblyEva M. Schmid. 1[, Marijn G. J. Ford. 1[, Anne Burtey. 2, Gerrit J. K. Praefcke. 3, Sew-Yeu Peak-Chew. 1, Ian G. Mills. 4,. Alexandre Benmerah2. , Harvey T.

27 Feb 2022: resistantgenes in the endogenous RNAi background.

30 May 1998: m. EDTA (pH 7.4) for 30 min at0. 8. C, then with micrococcal nuclease to reduce background trans-lation products due to endogenous mRNA (29).

14 Jul 2020: Approximately 24h endogenous biological oscillation that persists under constant conditions, whose period is temperature compensated. ... Approximately 24h endogenous biological oscillation that persists under constant conditions, whose period is

7 Mar 2012: To clarify, we obtained two FtsA crystal structures: a) the first one contained an FtsA dimer (each FtsA molecule had the FtsZ peptide bound and had an endogenous ATP molecule at

21 Dec 2004: A digitonin extract containing cytosolic proteins in-cluded the majority of endogenous NF-B (Figure 3B) aswell as several other cytosolic markers such as heat shockprotein (HSP)70 and HSP90 (our ... ng of plasmid (lane 1) was compared with endogenous

29 Dec 2004: currently assessing the role of endogenous duck erythrocyte phosphatases in regulating the cotransporter.

15 Sep 2020: Cells were arrested in G1 with a-factor prior. to release into auxin and nocodazole containing media at 25˚C to deplete the endogenous Scc3.

21 Sep 2006: As predicted, these mice are resis-tant to prion infection and propogation41,42, consistent with a require-ment for endogenous PrPC in the replication of PrPSc.

6 Feb 2003: The. Jour. nal o. f Cel. l Bio. logy. JCB. Article. The Journal of Cell Biology, Volume 160, Number 4, February 17, 2003 529–539http://www.jcb.org/cgi/doi/10.1083/jcb.200210095 529. Substrate-specific function of the translocon-associated protein

29 Aug 2021: Iba1) or 60 min at 37 C (anti-CD3, anti-CD4). This wasfollowed by blocking of endogenous peroxidase (peroxidase.

11 Nov 2011: stable cell lines expressing either wild-type PrP or osteopontin (Opn)-PrP at levels comparable to endogenous PrP in brain (Kang et al., 2006).

23 Nov 2004: In the supplementary material of the previous paper we showed that these puncta co-localisedwith endogenous endocytic components such as eps15 and dynamin.

23 Sep 2011: 5C), indicating that the competitive interactionwith endogenous endophilin occurred at the membrane.

31 Jul 2019: 2011) Endogenous Myc maintains the tumormicroenvironment. Genes Dev. 25, 907916.(14) Gabay, M., Li, Y., and Felsher, D.

15 Jan 2004: Prnp0/0, except for Tg(MoPrP)4053/Prnp/, in which the endogenous Prnp genewas maintained by breeding with FVB mice (Charles River Laboratories, Hol-lister, Calif.).

24 Jul 2020: Juszkiewicz et al., 2018; Mills et al., 2016). Here, collisions were incurred on endogenous haemo-.

25 Sep 2013: c) D.Mel-2 cells immunostained to reveal endogenous Syndapin (red), Tubulin ( green) and counterstained with DAPI toreveal DNA (blue). ... cleavagefurrow and their effect on cytokinesis upon overexpression inthe presence of endogenous Syndapin.

HIV is a retrovirus, meaning it has to copy its RNA genome into DNA in order to infect cells.

19 Jul 2011: Nature Reviews Molecular Cell Biology 12, 517 (2011). doi:10.1038/nrm3151

28 Sep 2007: 1e, endogenous EHD2 in various cell lines shows asimilar peripheral distribution5,14).

15 Apr 2004: These conclu-sions suggested that, in principle, the organization of multi-protein complexes could be assessed with antibody probes.Thus, if endogenous translocon components could be labeledwith dye-conjugated antibodies while

1 Aug 2010: LETTERS. A ribosome-associating factor chaperonestail-anchored membrane proteinsMalaiyalam Mariappan1, Xingzhe Li1,2, Sandra Stefanovic1, Ajay Sharma1, Agnieszka Mateja3, Robert J. Keenan3. & Ramanujan S. Hegde1. Hundreds of proteins are inserted

30 Nov 2006: Substrate-Specific TranslocationalAttenuation during ER Stress Definesa Pre-Emptive Quality Control PathwaySang-Wook Kang,1,4 Neena S. Rane,1,4 Soo Jung Kim,2 Jennifer L. Garrison,3 Jack Taunton,3. and Ramanujan S. Hegde1,1 Cell Biology and

5 Jan 2024: tag endogenous genes in human cells.

We develop biochemical methods to obtain protein-nucleic acid complexes involved in telomere maintenance pathways both from endogenous sources and by overexpression methods.

17 Oct 2007: EpsinR D342R D422R overexpression in COS cells. Mutation of these two residues affects the interaction of EpsinR with clathrin. The phenotype is less severe than the lipid binding mutant (D34G R67L). pointing again to the importance of the ENTH

n. The research team included collaborators from the MRC Centre for Medical Molecular Virology and the Retrovirus Research Laboratory, MRC University of Glasgow Centre for Virus Research.

25 Jun 2007: EpsinR D34G R67L D342R D422R overexpression in COS cells. Blue Channel -. Transferrin. Red Channel -. AP1. Green Channel -. D34G  R67L D342R D422R. Merged. Colours as before. Colocalisation of EpsinR D34G R67L D342R D422R (green) with markers (red),

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Published on. 11 August, 2016. HIV is a retrovirus, meaning it has to copy its RNA genome into DNA in order to infect cells.

25 Aug 2023: A Method for the Acute and Rapid Degradation of Endogenous Proteins..

23 Nov 2004: Endogenous SNX1 was enriched on tubularmembranes in close vicinity to endosomal vacuoles (Fig-ure 3). ... These data establish a role for endogenous SNX1in the retrieval of the CI-MPR from early endosomes tothe TGN.

27 Nov 2003: Merge (from epsin1 sup data). Endogenous EpsinR. Clathrin. Merge. Comparison of epsin1 and epsinR.

biological-timekeeping/","title":{"rendered":"Discovering the origins of biological timekeeping"},"content":{"rendered":". The existence of an endogenous daily clock in humans is well known: it is what drives the 24-hour

20 Jan 2022: Published on. 20 January, 2022. A schematic showing the effect of CRY1 on the relationships between localisation and intracellular mobility of endogenous fluorescently tagged PER2 as well as circadian rhythmicity of ... Real-time imaging of endogenous

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23 Apr 2024: His application of forward-genetic screens discovered the Human Silencing Hub ‘HUSH’, the critical transcriptional epigenetic repressor complex for silencing both newly integrated retroviruses and mobile endogenous retrotransposons.

22 Nov 2002: EpsinR: an AP1/clathrin interacting protein. involved in vesicle trafficking. Myc-tagged and endogenous epsinR versus epsinR-GFP. ... EpsinR-GFP versus AP1 and clathrin. There is good overlap between the perinuclear epsinR-GFP and endogenous AP1 and

This method is the first widely applicable protein degradation method that acts directly on the level of the endogenous protein.

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