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14 Aug 2023: http://haseloff.plantsci.cam.ac.uk (note: these pages are suitable for direct printing) Jim Haseloff, University of Cambridge.

14 Aug 2023: Biology will never be the same. The remarkable scientific success of biology in describing, explaining, and manipulating natural systems is so well recognized as to be a cliche—but the engineering application of that scientific knowledge is just

14 Aug 2023: Genomics, Epigenetics & Synthetic Biology. Part II Plant Sciences Module L1. Synthetic Biology: Prof. Jim Haseloff. Supplementary material:. Click here to find relevant papers as downloadable PDFs. Click here to find sample essay questions. Click

14 Aug 2023: Microscopy of GFP-expressing plant tissues. The major advantage of GFP is that it is intrinsically fluorescent, and the protein is be directly visible in living tissues where it is being expressed. There are many benefits to working with live samples

14 Aug 2023: Cambridge team for the iGEM2012 Synthetic Biology competition. Cambridge iGEM2012 team project. iGEM teams have characterised an array of biosensor components, but outputs are often inconsistent and, despite common assembly standards, it remains

14 Aug 2023: Macrophotography in the field. The documentation of liverworts in the field, growth room or laboratory can be a challenge. The small size of the plants means that plant features may need to be captured at sub-millimetre, almost cellular scales.

14 Aug 2023: The student team. Each year we recruit 6-12 undergraduate students to participate in the iGEM competition. The students work as an interdisciplinary team to construct an engineered biological system from DNA components, including design and

14 Aug 2023: Synthetic Biology Explained: Bridge8. Synthetic Biology: Overview - Victor de Lorenzo. Early Synthetic Biology talk: Tom Knight. E.O. Wilson: Synthetic Biology Will Radically Change the World. Jim Haseloff, University of Cambridge.

14 Aug 2023: Cambridge iGEM2011 Bactiridescence project. Inventive constructions using BioBricks. Bioluminescence and the science of Christmas. Glowing trees could light up city streets. How 'bioluminescent' trees that glow like fireflies could one day replace

14 Aug 2023: Plant and microbial microscopy and visualisation. The last twenty years have seen a revolution in the application of optical techniques to the study of biological systems. This largely due to the development of highly specific fluorescent labelling

14 Aug 2023: Plant Synthetic Biology. University of Cambridge, Plant Sciences Part II. Discuss the relative benefits and difficulties of genetically reprogramming metabolism in algae versus plants. Describe recent advances in the assembly of synthetic DNAs. What

14 Aug 2023: Plant Visions 1904-2004. An exhibition of images to celebrate the centenary of the opening of the Plant Sciences laboratories in 1904. Curated by Beverley Glover (website) and Jim Haseloff. Click here to view the exhibit. Download a copy of the

14 Aug 2023: 3D visualisation. Individual cells can be segmented using confocal datasets and rendered in three dimensions. Whole tissues can be reconstructed from the component cells - as shown here for a root meristem within an Arabidopsis embryo. Outer cells

14 Aug 2023: Abstract: Malaria is a global health problem that threatens 300-500 million people and kills more than one million people annually.

14 Aug 2023: Crystals, microbes and plants. Fernán Federici & Jim Haseloff. “What are the roots that clutch, what branches grow. Out of this stony rubbish?”. TS Eliot, The Waste Land. Part of a pioneering new exhibition, ‘April is the Cruellest Month.’

14 Aug 2023: Synthetic Genomics. A series of papers has been published in Science (10th March 2017), which provide a picture of current progress to resynthesise the entire genome of Saccharomyces cerevisiae - the SC2.0 project (). Four of these papers have been

14 Aug 2023: Vanguard Alta Pro 2+ 263AT with GH-100 pistol grip ball & socket head. For high resolution and/or time-lapse work with small plants like liverworts, a camera support is very useful…even essential. The camera support of course needs to be stable,

14 Aug 2023: Mk I Handheld Macroscope (Spring 2017). Original setup for high resolution handheld macrophotography. 1. Panasonic Lumix GX80 camera with 16 megapixel 4/3" sensor, image stabilisation, 4K video, focus bracketing and image stacking. 2. Neewer 10mm

14 Aug 2023: Weatherproof cameras for field documentation. Comparison of Olympus TG-4 and TG-5 cameras for handheld collection of extended focus macrographs. The TG-4 is an extremely robust point-and-shoot camera that can be equipped with an LED illuminator (LG-1

14 Aug 2023: The wild type Aequorea victoria green fluorescent protein mRNA is mis-spliced in Arabidopsis thaliana. GFP sequences were amplified from DNA or mRNA isolated from transgenic Arabidopsis thaliana C24 plantlets. Radiolabelled products were then mapped

14 Aug 2023: GFP background. Aequorea victoria are brightly luminescent jellyfish, with glowing points around the margin of the umbrella. Light arises from yellow tissue masses that each consist of about 6000-7000 photogenic cells. The cytoplasm of these cells

14 Aug 2023: Maturation of green fluorescent protein. The post-translational maturation of GFP to the fluorescent form involves a number of steps. In the first step, the GFP apoprotein presumably must fold into a catalytically active conformation that

14 Aug 2023: Jim Haseloff. email: jh295(at)cam.ac.uk. Haseloff Lab. Department of Plant Sciences. University of Cambridge. Downing Street. Cambridge CB2 3EA. United Kingdom. tel: 44-1223-766546. swb: 44-1223-333900. web: http://haseloff.plantsci.cam.ac.uk.

14 Aug 2023: Manuel Waller, Eftychios Frangedakis, Alan O Marron, Susanna Sauret-Gueto, Jenna Rever, Cyrus Raja Rubenstein Sabbagh, Julian M Hibberd, Jim Haseloff, Karen Renzaglia, Péter Szövényi. Plant Journal, doi: 10.1111/tpj.16161. 2023. Land plants

14 Aug 2023: Research projects in the Haseloff Laboratory. Synthetic Biology is an emerging field that employs engineering principles to construct new genetic systems. The approach is based on the use of well characterised and reusable DNA components, and

14 Aug 2023: Part 1B Cellular & Developmental Biology. Plant Development. Prof. Jim Haseloff, University of Cambridge. Summary:. These four lectures cover some striking features of biological self-organisation and morphogenesis using examples from the model plant

14 Aug 2023: Cambridge-JIC teams in the international Genetically Engineered Machine (iGEM) competition. iGEM is an international undergraduate synthetic biology competition where student teams are given access to DNA parts from the Registry of Standard

14 Aug 2023: iGEM2010 project: E. glowli. The Cambridge iGEM2010 team worked on regeneration systems for bioluminescence and better, brighter light output, different coloured luciferases, instrumentation for quantitation and modelling the feasibility of using

14 Aug 2023: iGEM2009 project: E. chromi. The Cambridge iGEM2009 team created two kits of parts to facilitate the design and construction of biosensors in the the future. These complement genetically engineered bacterial biosensors that enable bacteria to

14 Aug 2023: iGEM2006 project: Self-organisation. Multicellular organisms undergo self-organisation during development. Our aim was to engineer self-organised pattern formation in free swimming bacteria cells by providing them with an artificial system allowing

14 Aug 2023: iGEM2007 project: Bacteria-On-Line. In order to engineer interesting and useful functions in biology, a robust and extensive range of intra- and inter-cellular signalling pathways must be available. By analogy with the Internet, where adoption of

14 Aug 2023: iGEM2005 project. The Cambridge iGEM2005 team explored a method to control chemotaxis using maltose regulation. The Cambridge iGEM2005 wiki is at:. Background information is at:. iGEM 2005 Students. James Brown: (Engineering, Cambridge) & PhD in

14 Aug 2023: The liverworts (or Marchantiophyta) are descendants of the earliest terrestrial plants. The group is characterised by morphological simplicity, and this is matched by simple underlying genome structures. Many lower plants, including liverworts,

14 Aug 2023: iGEM2011 project. Reflectins - iridescent proteins. The Cambridge iGEM2011 team worked with bionanophotonic systems - experimenting with the use of self-assembling protein structures from squid that produce striking iridescence in vivo. They

14 Aug 2023: NST PMS 1B: Supporting materials. Prof. Jim Haseloff (jh295@cam.ac.uk). Links to supplementary lecture materials below:. Click here to download a copy of the lecture notes and extended material as a PDF document.. (126MB, 38 pages). Lecture 1. Plant

14 Aug 2023: Marchantia polymorpha - a new model system for Synthetic Biology. The liverworts (or Marchantiophyta) are descendants of the earliest terrestrial plants. The group is characterised by morphological simplicity, and this seems to be matched by simple

14 Aug 2023: iGEM2008 project: iBrain. The Cambridge iGEM2008 team project aimed to set a foundation for future research in engineered multi-cellularity by pursuing electrical and peptide signalling, and cellular self-differentiation through spontaneous spatial

14 Aug 2023: The ß-glucuronidase (GUS) gene is used extensively as a reporter for gene expression in plants. Transformed tissues or patterns of gene expression can be identified histochemically, but this is generally a destructive test and is not suitable for

14 Aug 2023: Wellcome Image Awards 2011. Special Award. (http://images.wellcome.ac.uk/). Arabidopsis thaliana seedling. Fernan Federici and Lionel Dupuy. Wellcome Image Awards 2011. Award. (http://images.wellcome.ac.uk/). Ergot infected wheat flower. Fernan

14 Aug 2023: http://haseloff.plantsci.cam.ac.uk (note: these pages are suitable for direct printing) Jim Haseloff, University of Cambridge.

14 Aug 2023: Agrobacterium tumefaciens and Plant Cell Interactions and Activities Required for Interkingdom Macromolecular Transfer. Colleen A. McCullen and Andrew N. Binns. Agrobacterium-mediated genetic transformation of plants: biology and biotechnology.

14 Aug 2023: Microscopy weblinks. Molecular Expressions website featuring photo galleries that explore the fascinating world of optical microscopy, with large collection of photomicrographs, and tutorials including diagrams and interactive Java applets:. Laser

14 Aug 2023: Reporter genes in plants. Self-assessment worksheet. Gene fusions, GUS, GFP and microscopy. Library/Internet exercise:. Answer the following questions:. 1. Draw a diagram of a typical protein-coding plant gene. The annotation should include the

14 Aug 2023: http://haseloff.plantsci.cam.ac.uk (note: these pages are suitable for direct printing) Jim Haseloff, University of Cambridge.

14 Aug 2023: 10. Invites Parties, other Governments, relevant organizations and indigenous peoples and local communities to submit to the Executive Secretary information and supporting documentation on:. ... indigenous peoples and local communities through paragraph

14 Aug 2023: chromi after atense online vote. Three days and 112 presentations of synthetic biological machines later, we found ourselves on stage,electrified, in front of 1,500 people in MIT’s largest

14 Aug 2023: People are trying things; kettles are exploding. Everyone’s attempting. magic right and left.”. ... A television. network named him one of Slovenia’s seven most influential people.

14 Aug 2023: the trait that causes fireflies to glow -bioluminescence - to be implanted into a variety of different organisms.As well as replacing traditional streetlights, bioluminescent plants would be useful for people who

14 Aug 2023: colias overtly harmless, or (if you've spent some time studying their metabolism and control thereof) particularly simple ;-). Otherwise, the iGEM and all the people taking part are made of WIN!

14 Aug 2023: Bioluminescent plants could appeal especially to people whose homes are not wired up to theelectricity grid.