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  2. Fluorescence Anisotropy Theory Method and Data Analysis

    https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/fluorescence_anisotropy_theory_method_and_data_analysis.pdf
    17 Nov 2023: Anisotropy measurements are based on the principle of selective excitation of fluorophores by polarised light. ... For this reason, free fluorophores in aquas solutions display anisotropy near zero (free fluorescein r = 0.0236).
  3. Fluorescence Anisotropy Theory Method and Data Analysis

    https://facilities.bioc.cam.ac.uk/files/media/fluorescence_anisotropy_theory_method_and_data_analysis.pdf
    17 Nov 2023: Anisotropy measurements are based on the principle of selective excitation of fluorophores by polarised light. ... For this reason, free fluorophores in aquas solutions display anisotropy near zero (free fluorescein r = 0.0236).

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