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pnas201210899 16522..16527
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2012-Aylett-16522-7.pdf12 Oct 2012: electronmicroscopy. We produced full-length, untagged Bt TubR andpurified it to homogeneity (Fig. ... Significantly thissuperstructure displays the positively charged DNA bindingsurface on the exterior of these helices (Fig. -
Current Developments in Coot for Macromolecular Model Building…
https://www2.mrc-lmb.cam.ac.uk/coot/protein-science/Casanal_Lohkamp_Emsley_11112019-pe-2.pdf11 Nov 2019: first try to solve a structure with heavy atom derivatives if molecular replacement. ... or are more flexible than the majority of the structure). Interactive display of the. -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: First, prepare the condition with initial concentrations of reagents increased by 10% (min. ... Table 4: Programs available on the microsyringe-based nanoliter dispenser for additive screening. -
201010176 1..6
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Aylett-TubZ.pdf21 Oct 2010: Results and DiscussionCrystal Structures of the TubZ Protofilament. We cloned and over-produced TubZ from B. ... 1B. TubZ possesses an addi-tional helical C-terminal tail, which is conformationally flexible. -
Mechanism of ribosome stalling during translation of a poly(A) tail
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/NSMB_2019.pdf25 Nov 2019: Fig. 2c and Extended Data Figs. 2 and 3). Based on biochemical analysis of stalling (Fig. ... When poly(A) first enters the A-site, it likely samples the helical state very rapidly. -
A Large Particle Associated with the Perimeter of the ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb82.pdf5 Jul 2010: The rings are composed ofglobularsubunits and their power spectra display weak eightfold com-ponents (results not shown). ... of the membrane borders produced by high salt or EDTA isassociated with the detachment of particles from around the. -
PII: S1097-2765(02)00515-4
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf19 Apr 2002: The hinge domains of these bacterialphase. SMC proteins are sufficiently flexible that the two head. ... In the first case, the N- and5 Present address: Center for Cancer Research, Massachusetts Insti-. -
Structure of a cholinergic cell membraneNigel Unwina,1 Edited by ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS22.pdf4 Nov 2022: 183. Distinct Lipid Organization in the Two Leaflets. To display thefeatures responsible for the profile in Fig. ... Cholesterol monolayer films were produced at the air-water interface, using60 μL (4 mm diameter) water-filled Teflon wells. -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: LeoABCshows splitting of the first gene into two as shown in Figure 1B and C. ... HHL JMJ JL. Wrote the paper: KAM HHL JL. First recognised LeoB/C. -
Bacterial chromosome segregation: structure andDNA binding of the Soj …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/soj_embo2005.pdf30 Jan 2005: displays no detectable ATPase activity, a consequence of its. failure to bind nucleotide. ... MinE: first 35 amino acids (N-terminal domain). FtsA:last 20 amino acids. -
Articleshttps://doi.org/10.1038/s41594-019-0196-z 1MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%202019%20NSMB.pdf4 Mar 2019: P. & Hirano, T. Condensin and cohesin display different arm conformations with characteristic hinge angles. ... N., Briscoe, G. & Erickson, H. P. The symmetrical structure of structural maintenance of chromosomes (SMC) and MukB proteins: long, -
tra_578 1..18
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Burtey%20IVF%20Traffic%2007.pdf15 May 2007: nitrocellulose membrane was first stained with ponceau red for quantification of GST fusion proteins. ... This hypothesis was. first verified in vitro by testing the AP-2-binding properties. -
doi:10.1016/j.molcel.2004.08.030
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf20 Sep 2004: Burley, 2000) that binds to the Smc1 head. The first 32the sort of dimeric structures seen in our crystals. ... type or noncleavable Scc1 was expressed under control onset of the first anaphase (Figure 5C). -
ELIFE07975 1..21
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/94_Voorhees_RM_eLife_2015.pdf11 Aug 2015: The cryo-EMreconstruction of this native complex provided the first structure of SRP bound to the ribosome ina ‘scanning’ mode. ... Integration of SRP recruitment with the translational elongation cycle. (A) Stalled RNCs were produced. -
2168 | K. von der Malsburg et al. Molecular ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf11 Aug 2015: First, the region around the ribosome exit tunnel is highly crowded when bound to the translocon (Pfeffer et al., 2014). ... A) Short RNCs containing the pPL or N7a signal peptides were produced in reactions containing or lacking RMs. -
15211199233993 1..45
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreBHussain2018.pdf15 Mar 2018: with tagO under inducible control display a teichoic acid-dependent decrease in width. ... cell contours (Figure 1—figure supplement 1B), suggesting the wall was becoming more flexible. -
doi:10.1016/j.cell.2006.03.035
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16647110.pdf14 Jun 2006: Based on these cocrystal structures and a conserved. arylmorpholine pharmacophore model, structural models. ... These pioneering. studies were among the first to explore signaling by PI3-K. -
Structure of a Bacterial Dynamin-like Protein Lipid Tube Provides a…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cell%202009%20dynamin.pdf19 Dec 2009: We fittedthe BDLP crystal structure and produced a molecularmodel for the entire filament. ... First, BDLP, which probably exists as dimer. in solution, is loaded with GTP (Figure 7A). -
A Polymerization-Associated Structural Switch in FtsZ That Enables…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf3 May 2017: First, FtsZ exhibits cooperative assembly, with a critical concentrationand a lag phase in assembly. ... situation of net growth at one end and netshrinkage at the other can be produced at certain monomer concentrations (additionreaction is first order -
Protein targeting and degradation are coupled for elimination of…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/73_Hessa_T_Nature_2011.pdf14 Jul 2011: We produced a translation-competent fractionated RRL (Fr-RRL) (Supplementary Fig. 7) that selectively decreased the ubiquitina-tion of non-translocated PrP (Fig. ... To determine when the Bag6 complex first captures MLPs, we ana-lysed ribosome-nascent -
doi:10.1016/j.str.2008.06.010
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/18786397.pdf3 Sep 2008: First, the B molecules. from opposite strands contact each other at the center of the. ... The insets display schematic representations of. these interactions. (B) The intrastrand contacts between the a3/a4. -
ANRV378-BI78-31.tex
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DohertyMcMahonAnnRevBiochem09.pdf27 Apr 2009: Clathrin-independent endocy-tosis itself has been further dissected into seem-ingly distinct pathways, based on the reliance ofthese pathways on certain proteins and lipids,their differential drug sensitivities and theirabilities ... Eps15 appears to act
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