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12 Oct 2002: To test this notion, wemeasured the binding of mutants to vesicles contain-ing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidic acid(POPA) or

13 Apr 2015: To test this idea, we per-formed crosslinking analyses before and immediatelyafter incubation with membrane vesicles. ... To test this idea directly, we examined TRC40 interac-tions with Sec61b constructs appended with cyan fluores-cent protein (CFP) at

14 Jun 2007: Structural insight into the ESCRT-I/-II link and itsrole in MVB trafficking. David J Gill1,, Hsiangling Teo1, Ji Sun2,Olga Perisic1, Dmitry B Veprintsev3,Scott D Emr2 and Roger L Williams1. 1MRC Laboratory of Molecular Biology, Medical Research

27 Jan 2018: MEMBRANE TARGETING. The ER membrane protein complex isa transmembrane domain insertaseAlina Guna,1 Norbert Volkmar,2 John C. Christianson,2 Ramanujan S. Hegde1†. Insertion of proteins into membranes is an essential cellular process. The

28 Feb 1998: nents may enhance or otherwise modulate pausing insome-membrane junction.To test this hypothesis directly, we replenished the subtle ways not detected by the assays employed.

6 Apr 2005: sub-units are close to each other.23 To test this possibility,we chose our best map from RCCs made withpurified Sec61 and a nascent chain6 (Figure 2(d)), inwhich a

23 Nov 2004: Refinement statisticsR factor (%) 0.177RFree (%) 0.224RFree test set size (%) 0.051<B> Å2 (mean, overall) 27.98Nreflections (Nfree) 27464 (1467)Natoms (Nwater) 2467 (297)Rmsd bond length (Å) 0.03Rmsd

28 Sep 2007: To test if a polybasic stretch close to the tip of the helical domainwas involved in lipid binding, we monitored lipid binding of K327D,K328D and also F322A (Fig.

24 Jan 2002: To test this idea more directly, we compared the bio-(A) The Ang, Ifn-, or Prl signals fused to the PrP reporter (from genesis of Ang, Prl, PrP, and GH when

19 Nov 2019: Not significant (n.s.) and p < 0.001 () in paired Student’s t tests are indicated.

3 Aug 2009: To test this, 24 Get3 variants with single aspartate substitutions forhydrophobic residues on the surface of the groove were analysed forsubstrate interaction in vitro and functional complementation of aDget3 yeast strain

20 Nov 2010: Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test.

31 Aug 2006: To further narrow the interaction sequence wetested a number of peptides (previously made to test theinteraction with the a-appendage) and found peptides thatcan interact with the b-appendage (Eps15

31 Jul 2019: acid, 0.75% gallic acidmonohydrate, and 0.75% N-vanillylnonanamide] (plate LMB22, an in-house test formulation of the MORPHEUS IIIcrystallization screen); crystals of Collect 2/PDB entry 6G6J,20% PEG

18 Feb 2016: Thus, the idea emerged that mRNAs are ‘tested’ for their integrity by translation, and failure of this test initiates their degradation to prevent production of faulty proteins1,31.

5 May 2019: To test this further, we investigated the bindingof the periplasmic domain of FtsN to immobilized FtsQ as a control (Fig. ... S5]). Because of the very low level of FtsB incells and the low protein levels needed to complement in our assays, we have

15 Sep 2020: We did not test. the effect of mutating the equivalent residue in Smc1 as this has previously been shown to abolish. ... supplement 1C). To test whether these residues participate in binding DNA, we used EMSAs to.

15 Aug 2007: Molecular Biology of the CellVol. 18, 3681–3691, September 2007. The Role of p58IPK in Protecting the Stressed EndoplasmicReticulumD. D. Thomas Rutkowski,† Sang-Wook Kang,†‡ Alan G. Goodman,Jennifer L. Garrison, Jack Taunton, Michael G. Katze

15 Sep 2011: MOLECULAR BASIS FOR MEMBRANE REMODELLING  AND ORGANIZATION . Analyses moléculaires de l'organisation et du remodelage des membranes. President: Harvey McMAHON MRC Laboratory of Molecular Biology, Neurobiology Division,.

20 Jun 2009: Horizontal black barsindicate the mean values for each data set. Each mutant dataset was compared to wtPrP by the Student’s t-test and found to be statisticallysignificant in all cases

24 Jul 2020: To test this idea, we monitored translation output from a reporter mRNA containing a collision-. ... Hegde, 2017). This precluded a clear test of EDF1’s role using this stalling sequence.

20 Jul 2005: test this hypothesis, we analysed b5-Nglyc translocation. into protein-free liposomes of differing composition.

15 Dec 2021: To test the sensitivity of these results toour choice of queries (6fti N and 6itc C), selected abovefor maximum symmetry, we repeated them with the op-posite half of each structure

28 Sep 2012: If you set it, Coot will test each ’:’-separatedstring that it points to a directory, and if it does, Coot will load all the. ... This variable is not set by default. If you set it, Coot will test each ’:’-separatedstring that it points to a

12 Jun 2020: To test. whether substrate binds in this cavity, we prepared the EMC2.EMC9 complex containing the photo-. ... mate binding site that spans both EMC2 and EMC9. To test the functional relevance of the vestibule, we perturbed its physicochemical properties

14 Jul 2021: decreasing it with Pds5. To test this, we measured the effect of scc4-4 in the presence or absence of.

18 Sep 2008: In this study, we have. performed experiments to test these predictions.

11 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,.

22 Jul 2008: We. analyzed subsets of the final data set to test this idea.

11 May 2007: UNIT 17.9Rational Design and Evaluation of FRETExperiments to Measure ProteinProximities in Cells. Defining the repertoire of interactions thata particular protein can undergo is crucialfor understanding its function and regulation.Characterizing

6 Dec 2004: Data Analysis—Data are given as means S.E. Comparisons be-tween means were performed using one-tailed Student’s t test forpaired data with GraphPad PrismTM software.

21 Dec 2004: Differences among eachconstruct were statistically significant (p 0.01 by Student’s t test).(B) Ten nanograms per well of constructs encoding the indicatedsignal sequences fused to TF were analyzed for

3 Feb 1997: To test thesepredictions, we blocked assembly in a variety of ways,both pharmacologically and through the use of mutations,and examined the Gag-containing complexes that wereformed.

17 Aug 2020: To test this, we determined whether PAT complex interaction is selec-tive for immature rather than folded membrane proteins.

11 Aug 2015: To test this idea, scanning and engagedHA-tagged RNCs were assembled in the pres-ence of FLAG-tagged Hbs1-DN, affinity purifiedvia the FLAG tag, and tested for SRP recovery(Figure

11 Nov 2011: Molecular Biology of the CellVol. 21, 4325– 4337, December 15, 2010. Compartment-Restricted Biotinylation Reveals NovelFeatures of Prion Protein Metabolism in VivoAmy B. Emerman, Zai-Rong Zhang, Oishee Chakrabarti,†and Ramanujan S. Hegde. Cell

9 Sep 2015: Comparison of sensitive and resistant TMDs at multiple points along the integration pathway (i.e., at multiple nascent chain lengths) should allow us to: (1) test whether they pass through a

25 Sep 2013: kDa DM. el. fly. brai. n. test. is. tota. l. SyndTub.

24 Aug 2011: 6 F). To test the requirement for CLIC endocytosis during mi-gration, this inactivation assay was applied to migrating fibro-blasts.