Search

17 Jan 2012: Bars and error bars correspond to mean and SEM calculated from three independent experiments (n > 250; α = 0.05; two-tailed Fisher’s exact test, , p < 0.001). ... Bars and error bars correspond to mean and SEM (n = 12–15; α = 0.05; Student’s t

23 Sep 2011: AnANOVA test confirmed that the amplitude of the fast phase wassignificantly reduced by endophilinSH3 (p 0.01). ... jneurosci.org as supplemental material), soit was important to test the specificity of its effect.

22 Jun 2001: To test this idea, we directly comparedthe translocation efficiencies of pPrl containing the pPrl, pbL, orpIgG signal sequences. ... translocation efficiency of bL-Prl and IgG-Prl. To test thishypothesis, we added either the first 5, 54, or 117 aa of the

1 Nov 2012: Acid-washed glassware was used in experiments designed to test sensitivity o f gap j u n c t i o n structure to divalent cations.

11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated.

3 Sep 2014: DLPs. We attempted to test this hypothesis directly but were. unable to obtain either individually, in combination, or expressed.

11 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,.

29 Aug 2021: outside of the sphere. We aimed to test whether Dps could efficiently dis-. ... Spike-S-Dps sera gave very little neutralisation. Whilst. pseudoviruses are widely used to test the neutralisation.

23 Dec 2013: To test whether the translocase is responsiblefor promoting and maintaining the hexameric state of FtsK invivo, we determined the stoichiometry of an FtsK derivative lack-ing the C-terminal domain (FtsKC;

22 Aug 2007: We would suggest that a good test for proteins that function ashubs only when clustered in space and time is that overexpression ofsuch a protein should not have any phenotype on

19 Apr 2002: To test whether Smc3 s head alone is sufficient. to bind Scc1, we created an artificial head in which25 min, whereas the on rate of Smc3 was about twicethat of its ... To test this directly, we repeated the aboveexperiment using a yeast strain expressing

15 Apr 2004: Each bar represents the mean SD (n 10), with statistically significant differences (P 0.01 using t test) being observed between the two conditions. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test.

11 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,.

21 Nov 2014: S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin ring entraps sister DNAs in a co-valent topological embrace, we isolated dimersof a

17 Jun 2005: To test if a peptide de-rived from rat amphiphysin would inhibit the amphi-physin-AP2 interaction in goldfish, we performed abinding experiment using a GST-tagged construct of ratamphiphysin 1 ... ease of vesicles that were not primed at the time

5 Dec 2012: To test the biological relevance of Gbg-mediated activation in p110bsignaling, we compared the ability of wild-type and mutant p110bconstructs to mediate cellular transformation and motility. ... To test the role of p110b-Gbginteractions in PTEN-null

17 Mar 2003: test set of reflections, consisting of a randomly selected 5% of the data and not used during refinement.

26 Jan 2006: 1E)(Ford et al., 2002). As many other proteins are predicted to containN‐terminal amphipathic helices, the liposome tubulation assay will provea valuable test for the folding and insertion of these

6 Apr 2017: The model was improved manually using MAIN(ref. 38) and refined with PHENIX and REFMAC (refs 39, 40), keeping the 5%randomly selected test set of reflections separate at all times.

8 Sep 2006: In tests for MT. binding, the Kar3 protein showed a Kd of 0.37 6 0.10 mM with no.

22 Aug 2007: sufficient overexpression to test if the network can be distorted and overexpressed.

22 Mar 2005: In contrast,EE/RE, was inhibited in CHC RNAi-treated cells. Welarge amounts of STxB/HRP were found in clathrin-con-used quantitative biochemical techniques to test thistaining early endosomal vacuoles ... To test whether AP1 is required for the retro-

17 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. •

2 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour

19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.

11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... To test this, we treated cultured cells with either the transla-tion elongation inhibitor cycloheximide (CHX)

8 Dec 2007: To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.

29 Aug 2012: The crystal structure of the complex between BETA and BLIP has been a test case for molecular replacement because of the difficulty encountered in the original structure solution.

17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding.

16 Oct 2009: Future geneticstudies will allow us to test this possibility. References and Notes1.

4 Apr 2003: The best estimate was taken to be the defocus yielding the highest image Quality, Qi, de- fined as the number of test phases in agreement with (Iphase difference1 < 90’) the centrosymmetric ... 13). To test how sensitively PQ measures the signal-to-

13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.

30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel

15 Jul 2005: 2e). To test whether this difference underlies cotransin’ssubstrate selectivity, we prepared chimaeric constructs in which thesignals of VCAM1 and pPrl were fused to an otherwise non-translocated reporter. ... 3a), these results suggestthat cotransin

18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig.

13 Jun 2009: It will then be possible to test in transgenicmice whether the effects of the HD mutant stem solely fromCtmPrP production or through other mechanisms (e.g. ... PrP gener-ation, trafficking and degradation so that they can bemanipulated selectively to

12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... To test the impact of PSTPIP2-deficiency disease in mice onosteolytic responses in vivo, we performed

4 Oct 2010: Thus, our first goalwas to test whether the overall structure of the endophilin BARdomain is retained upon membrane interaction and, if so, howits concave surface interacts with the membrane. ... To test for potential movement between thecentral region

4 Mar 2008: Expression tests were done inBl21AI cells (Invitrogen) and C41 cells (Miroux and Walker,1996) with a 10 ml culture and purified over a Ni2 NTA spincolumn as described previously (van den

13 Jun 2009: 2.4. Northern blotting. RNA was extracted from cells using RNA STAT-60 (Tel-Test Inc.,Friendswood, TX). ... 2A). To test the functionality of the R383A and R383G mutants, flowcytometry was performed after incubating cells with the ABCG2-substrates

3 Sep 2008: some uncertainty of the molecular interpretation of the filaments,. so we carried out site-specific mutagenesis to test the contacts.

9 Nov 2001: For complementation tests, amph26 homozygoteswere crossed to Sin3A/CyO mutant stocks, and non-curlyprogeny were assessed for amph26 mutant phenotypes.

23 Jan 2012: Three-dimensional Structure of the Acetylcholine Receptor by Cryoelectron Microscopy and Helical Image Reconstruction C h i k a s h i T o y o s h i m a a n d N i g e l U n w i n. Medical Research Council Laboratory of Molecular Biology, Cambridge

15 May 2007: To directly test the. role of each residue of the IVF motif, in the context of. ... Methods. Errors bars were obtained by calculating the standard error and relevance of the difference assessed with a Student t-test.

23 May 2012: Wewere unable to test Trc35 in this assaybecause we could not purify Trc35 dueto poor expression and solubility. ... To test the involvementof the Bag6 complex in this process, we treated permeabilizedA9 cells with high salt, which removed most

30 Jul 2002: To test this hypothesis, HeLa cellswere fixed and stained for Eps15 (Figures 4A and 4B)and both AP1 and AP2 (Figures 4C and 4D) using anantibody against 1- and 2-adaptins.

16 Jul 2008: To test the involvement of thispathway in PrP degradation by A4 cells, we analyzed PrPmetabolism upon inhibition of ERAD at two distinct stepswith either a proteasome inhibitor (MG132) or mannosidaseI inhibitor ... To test this idea, we generated a

1 May 2004: At least theparC/ParR/M system is simple enoughto test this possibility in vitro.

7 Mar 2012: The reason for this is probably that the C-terminal amphipathic helix needs to be in contact with the membrane for polymerisation to occur at test-tube concentrations and in the ... Obviously, there are limitations as to what is possible, determined by

19 Dec 2016: crenactin filaments. To test this, we performed. the same experiment using a peptide spanning.