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18 Apr 2006: through an adaptor protein. To test whether these two. complexes can directly interact in vitro, we used analyt-. ... Avanti or Echelon). Control vesicles contained an additional 10% PC. instead of the test lipid.

11 Apr 2024: signalling pathway in the Drosophila midgut. Development 122, 849-858. 47. Hart K & Bienz M (1996) A test for cell autonomy, based on di-cistronic messenger.

17 Mar 2003: amplitudes. In contrast, EPSC amplitudes of synchronous release (Geppert et al., 1994).in double mutant cells increased during the first few To test the possibility that CPX I/II deficiency causesstimuli ... Third, maximal values of synchronoussynaptic

11 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,.

30 Apr 2010: To test the influence of EGTA and BAPTA on spontaneous release, spontaneous events were recorded first in naïve cells during 1 min, followed by a EPSC recording after stimulation. ... The spontaneous release frequencies before and after loading of a Ca2+

4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely.

3 Sep 2014: DLPs. We attempted to test this hypothesis directly but were. unable to obtain either individually, in combination, or expressed.

1 Nov 2012: Acid-washed glassware was used in experiments designed to test sensitivity o f gap j u n c t i o n structure to divalent cations.

19 Aug 2023: To test whether internal loop translocation occurs at the secretory translo-con, we analysed biogenesis intermediates of EAAT1, an eight-TMD protein28 with a glycosylated luminal loop after TMD3 (Fig. ... Statistical analyses in e,g were performed by

16 Aug 2008: loading of a FtsK hexamer upon interaction with KOPS. This assay provides a good test for efficiency of loading of. ... sequently activate recombination. In order to test whether the dimeric forms of the protein had.

27 Feb 2022: To test this, we compared the affinity ofNAC for RNCs displaying either an ER signalsequence (RNCSS) or a mutated signal sequencethat inhibits ER targeting (RNCSSmt) (fig. ... S17, B and C). Thus, thecontact between NAC UBA and SRP54 NG do-mains

2 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour

23 Dec 2013: To test whether the translocase is responsiblefor promoting and maintaining the hexameric state of FtsK invivo, we determined the stoichiometry of an FtsK derivative lack-ing the C-terminal domain (FtsKC;

29 Aug 2012: The crystal structure of the complex between BETA and BLIP has been a test case for molecular replacement because of the difficulty encountered in the original structure solution.

17 Jan 2012: Bars and error bars correspond to mean and SEM calculated from three independent experiments (n > 250; α = 0.05; two-tailed Fisher’s exact test, , p < 0.001). ... Bars and error bars correspond to mean and SEM (n = 12–15; α = 0.05; Student’s t

22 Aug 2007: We would suggest that a good test for proteins that function ashubs only when clustered in space and time is that overexpression ofsuch a protein should not have any phenotype on

22 Jun 2001: To test this idea, we directly comparedthe translocation efficiencies of pPrl containing the pPrl, pbL, orpIgG signal sequences. ... translocation efficiency of bL-Prl and IgG-Prl. To test thishypothesis, we added either the first 5, 54, or 117 aa of the

4 Mar 2008: Expression tests were done inBl21AI cells (Invitrogen) and C41 cells (Miroux and Walker,1996) with a 10 ml culture and purified over a Ni2 NTA spincolumn as described previously (van den

1 May 2004: At least theparC/ParR/M system is simple enoughto test this possibility in vitro.

5 Dec 2012: To test the biological relevance of Gbg-mediated activation in p110bsignaling, we compared the ability of wild-type and mutant p110bconstructs to mediate cellular transformation and motility. ... To test the role of p110b-Gbginteractions in PTEN-null

19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.

9 Nov 2001: For complementation tests, amph26 homozygoteswere crossed to Sin3A/CyO mutant stocks, and non-curlyprogeny were assessed for amph26 mutant phenotypes.

19 Dec 2016: crenactin filaments. To test this, we performed. the same experiment using a peptide spanning.

11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... To test this, we treated cultured cells with either the transla-tion elongation inhibitor cycloheximide (CHX)

30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig.

4 Oct 2010: Thus, our first goalwas to test whether the overall structure of the endophilin BARdomain is retained upon membrane interaction and, if so, howits concave surface interacts with the membrane. ... To test for potential movement between thecentral region

13 Dec 2008: 5). To test the sensitivity of the model. predictions to this assumption, we repeated the calculations.

20 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly).

13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.

15 Jul 2005: 2e). To test whether this difference underlies cotransin’ssubstrate selectivity, we prepared chimaeric constructs in which thesignals of VCAM1 and pPrl were fused to an otherwise non-translocated reporter. ... 3a), these results suggestthat cotransin

30 Jul 2002: To test this hypothesis, HeLa cellswere fixed and stained for Eps15 (Figures 4A and 4B)and both AP1 and AP2 (Figures 4C and 4D) using anantibody against 1- and 2-adaptins.

3 Sep 2008: some uncertainty of the molecular interpretation of the filaments,. so we carried out site-specific mutagenesis to test the contacts.

24 Jan 2005: The model-derivedphases provided a more sensitive test of the signalretained in the images at high resolution than theprevious reference, which had been derived solelyfrom the images.

13 Jun 2009: It will then be possible to test in transgenicmice whether the effects of the HD mutant stem solely fromCtmPrP production or through other mechanisms (e.g. ... PrP gener-ation, trafficking and degradation so that they can bemanipulated selectively to

12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... To test the impact of PSTPIP2-deficiency disease in mice onosteolytic responses in vivo, we performed

17 Mar 2003: In order to test the validity of this hypoth-by six residues to complete the b strand, resulted in a.

13 Jun 2009: 2.4. Northern blotting. RNA was extracted from cells using RNA STAT-60 (Tel-Test Inc.,Friendswood, TX). ... 2A). To test the functionality of the R383A and R383G mutants, flowcytometry was performed after incubating cells with the ABCG2-substrates

15 May 2007: To directly test the. role of each residue of the IVF motif, in the context of. ... Methods. Errors bars were obtained by calculating the standard error and relevance of the difference assessed with a Student t-test.

16 Oct 2003: To test this, we fused GFP or YFP to thecytoplasmic domains of three resident ER membrane proteinswith minimal cytoplasmic domains: two rough ER com-ponents of the translocon, Sec61. ... chimera. To test if OSER-inducing, GFP-tagged ER proteinswere

20 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly).

7 Jul 1999: The residuals betweenthe tted absorbance and the experimental values wereplotted, to test for the validity of the model used.

4 Aug 2017: Fig. 2. Reconstitution ofUBE2O-mediated clientubiquitination with purifiedfactors. (A) Experimentalstrategy to test UBE2O inter-action and ubiquitination in adefined system.

21 Sep 2006: Meanwhile, the most definitive test for prions, a bioassay, was be-ing performed in parallel on comparable samples3639. ... These toolscan then be used in vivo to test specific hypotheses regarding the patho-genesis of prion diseases.

1 Nov 2004: An analogous salt link is formed betweenattractive proposal. To test this, we made an N-terminalArg83 of Vps25 and Asp548 of Vps36.

29 Dec 2004: In order to test whether stimulated [3H]bumetanide binding would be re- tained in membranes after activation of transport by agents other than catecholamines, we examined specific binding in membranes from

27 Apr 2015: To test for seeding, we followed filament formation onimmobilized TubRC complexes (Fig.

30 May 2008: Experiments must now be designed to test this hypothesis. Translocational pausing has been viewed as a mecha- nism by which particular regions of the nascent chain can be isolated from the

20 Nov 1997: Although the signifi-. cance of these findings remains to be determined, the Indeed, the contradicting conclusions resulting frommany of the studies which each used different test sub-rearrangements of the

21 Nov 2012: Oncogene. Correlation of lipid kinase activity with lipid bindingTo test whether lipid binding forms the basis of p110activation, we compared lipid kinase with lipid-bindingactivities for three sets of p110a/p85a

7 Apr 2010: FEBS Letters 584 (2010) 1830–1839. j o u r n a l h o m e p a g e : w w w. F E B S L e t t e r s. o r g. Review. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains. Felix Campelo a,b, Gur Fabrikant a, Harvey T. McMahon c,