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  2. Changes in Postural Syntax Characterize Sensory Modulation and…

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-5.pdf
    14 Aug 2015: A rank sum test comparingposture probabilities during and outside of stimulation periods shows that dorsal bends areover-represented (Fig 3C) during stimulation, as previously reported for this strain [21]. ... Hits are trigrams with significantly
  3. Identifying proteins bound to native mitotic ESC chromosomes reveals…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf
    11 Aug 2020: P-values of statistically significant increases, measured by unpaired two tailed Student’s t-tests, are indicated. ... P-values of statisticallysignificant decreases, measured by unpaired two tailed Student’s t-tests, are indicated.
  4. 73687_Trans

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099s2.pdf
    28 Apr 2010: Therefore, if F17 does not insert into the membrane bilayer (see above) the authors should test possible effects of the F17V mutation on DivIVA higher-order oligomerization by analytical ultra centrifugation. ... It should be straightforward to test this
  5. 1 Supplementary data to accompany paper: Mechanism of endophilin ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupMethods.pdf
    8 Jun 2006: simple gaussian functions to dc/dt versus s curves to test for the number of components and. ... Rfree test set size (%) 5.1 % outliers 0.5. <B> (Å2) 69.
  6. Tutorial 5: Hypothesis TestingRob Nicholls –…

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/hypothesis_testing.pdf
    26 Mar 2014: The F-test tests the null hypothesis that the variancesof two samples are equal using the test statistic:. ... 3 One-sample tests. One-sample t-test. The one-sample t-test tests the null hypothesis that the population mean is equalto some value µ0.
  7. The ER membrane protein complex promotes biogenesis of sterol-related …

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/JCS_2019.pdf
    18 Jan 2019: are shown (n=4). P0.01, P0.001 (Student’s t-test). 3. RESEARCH ARTICLE Journal of Cell Science (2019) 132, jcs223453. ... are shown (n=3).P0.001, P0.0001 (Student’s t-test). Reconstitution of Δ5 (D) and Δ6 cells (E) with empty vector control (EV),
  8. © 2005 Nature Publishing Group Endophilin and CtBP/BARS are ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndopohilinNature2005.pdf
    4 Jan 2006: Thus, a differentapproach is needed to test whether endophilin or CtBP/BARS doindeed have LPAAT activity.In a previous study1, the LPAAT activity of endophilin was. ... To test the possibility that LPAAT activity is not intrinsic toendophilin, we used a
  9. Elementary hypothesis testing •  Introduction •  Some distributions…

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/lectures/Lecture5_hypotheses.pdf
    7 Apr 2014: multiple hypothesis var.test - test for equality of variances power.t.test - to calculate power of t-test Some other tests. ... of two random variables are same Grubbs testtests for outliers (more in R package – outliers).
  10. Tutorial 5: Hypothesis TestingRob Nicholls –…

    https://www2.mrc-lmb.cam.ac.uk/download/tutorial-5-hypothesis-testing/?wpdmdl=18817&refresh=66816662556e91719756386
    26 Mar 2014: The F-test tests the null hypothesis that the variancesof two samples are equal using the test statistic:. ... 3 One-sample tests. One-sample t-test. The one-sample t-test tests the null hypothesis that the population mean is equalto some value µ0.
  11. The structure of human thyroglobulin

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf
    31 Jan 2020: Nature, doi:10.1038/s41586-020-1995-4
  12. se060101051p

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/ford01.pdf
    3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared
  13. Thesis Chapter 0

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Hardaker.pdf
    10 Jun 2008: To test the possible involvement of FLP-1-encoded peptides in the modulation of egg-.
  14. MD1-91, MD1-92 and MD1-92-FX Morpheus II 10 mL, HT-96 and FX-96…

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB20.pdf
    9 Jul 2018: Figure 1. Examples of crystals obtained during initial tests with the final formulation of Morpheus II.
  15. MD1-46, MD1-47 and MD1-47-FX Morpheus 10 mL, HT-96 and FX-96…

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB17.pdf
    9 Jul 2018: Preliminary tests with Morpheus made within the Laboratory of Molecular Biology (LMB)1 at Cambridge, UK, have shown encouraging results with various targets.
  16. httDownloaded from rsif.royalsocietypublishing.org ResearchCite this…

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-1.pdf
    4 Feb 2015: 2.12. Statistical analysisStandard statistical tests were performed using MATLAB func-tions. Correlation coefficients were calculated using theMATLAB function corrcoef. ... A value of þ1 indicates perfect positivecorrelation. A value of 21 indicates
  17. M410_8e 231..235

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynamin.pdf
    6 Mar 2001: In an effort to distinguish betweenthese two hypotheses, we designed mutants of dynamin that wouldenable us to test the relationship between dynamin's GTPase activityand its ability to support endocytosis. ... We then embedded cells asdescribed23. To
  18. se060101051p

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/ford.pdf
    3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared
  19. Tutorial 6: Linear RegressionRob Nicholls –…

    https://www2.mrc-lmb.cam.ac.uk/download/tutorial-6-linear-regression/?wpdmdl=18818&refresh=6681666292ffa1719756386
    31 Mar 2014: This essentially tests whether the. Tutorial 6: Linear Regression 4. model has any predictive power. ... b) Create a model to test this hypothesis, assuming a linear relationshipbetween Height and Girth.
  20. pgen.1004082 1..13

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/84_Chen_C_PlosGen_2014.pdf
    24 Sep 2015: indicatesp,0.01 by ANOVA, Bonferroni’s multiple comparisons test.(TIF). Figure S6 ODR-10-GFP localization in mutants expressingdefective rab proteins. ... p,0.01 (ANOVA, Bonferroni’s multiple comparisons test).(TIF). Figure S7 Deleting ufc-1 or uba-5
  21. Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf
    4 Jan 2006: This software was also used for the direct fitting of simple gaussian functions to dc/dt versus s curves to test for the number of components and estimate their molecular mass.
  22. 61 Ultramicroscopy 25 (1988) 279-292 North-HoUand, Amsterdam 279…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram88.pdf
    1 Apr 2016: In this paper, we examine the behaviour of the CTF for thin ice-embedded specimens within the framework of the hnear theory of image forma-tion, using as a test object ... Test calcula-tions showed that the accuracy of this method is not sensitive to
  23. pnas201014665 19609..19610

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Nogales-19609-10.pdf
    11 Nov 2010: 3). Just test your knowledge ofcytoskeleton structure by looking at theirfigure 4.
  24. pnas201313978 4601..4610

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas2013duman.pdf
    23 Dec 2013: characteristic of membrane-binding amphipathichelices (30, 31).To test whether this amphipathic helix is able to recruit pro-. ... To test functionalities of the SepF mutants, the sepF mutationswere then introduced into a B.
  25. DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf
    6 Apr 2007: Fur-ther work will be required to test this prelim-inary interpretation. Existing data indicates that ATP binding hasprofound effects on the kinesin head, recon-figuring the active site and shifting
  26. se060101047p

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Itoh%20et%20al.pdf
    23 Nov 2004: MBP asa test substrate in a 50-ml reaction. ... Statistical comparisonswere made with the two-way analysis of variance(ANOVA) and two-tailed t test with Bonferroni cor-rection; P , 0.05 indicated statistical significance.
  27. Features critical for membrane binding revealed by DivIVA crystal…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf
    20 May 2010: membrane interaction. To test this, we purified the F17A. mutant and investigated membrane binding in vitro. ... could conveniently test suppressors for restored localization. The motivation for such screen was that suppressors of the.
  28. Membrane Localization is Critical for Activation of the PICK1 BAR…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf
    18 Oct 2008: internalized to the observed clusters. To test this, we. performed an antibody feeding experiment using FLAG–. ... These values were significantlydifferent (p < 0.0001, unpaired t-test). B) COS-7 cells expressing.
  29. The structure of bactofilin filaments reveals their mode of membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf
    26 Aug 2019: Nature Microbiology, doi:10.1038/s41564-019-0544-0
  30. doi:10.1016/j.pbiomolbio.2004.07.009

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf
    22 Sep 2017: B) Aspiration of reagents from 15 ml test tubes held in a cooled rack. ... the desired chemical/buffer from 15ml test tubes held in arack on the robot platform.
  31. 53091 361..366

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf
    3 Dec 2004: Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of the test lipid (in most casesphosphatidylinositol(4,5)bisphosphate).
  32. pnas201206839 17424..17429

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/23045692.pdf
    21 Nov 2012: 2C and Fig. S2A, median abscission time of 70 min versus80 min for control treated cells; P < 0.0001, Mann–Whitneyu test). ... The differences in abscission times are significant (P < 0.0001) as determined by a Mann–Whitney u test.
  33. Twinning and other pathologies Andrey Lebedev CCP4 OD-­‐structures…

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/pathologies_and_twinning.pdf
    11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.
  34. doi:10.1016/j.cell.2006.03.035

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16647110.pdf
    14 Jun 2006: pounds and also test the prediction that distinct regions. of the ATP binding pocket contribute to inhibitor selectivity. ... cell culture are recapitulated in vivo by performing insulin. tolerance tests in mice.
  35. doi:10.1016/j.str.2007.05.002

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf
    2 Jun 2007: protein saturation on liposomes. To test more directly whether the dimer seen in the crys-. ... tutive helix. To test for a role for helix 1 in membrane bind-.
  36. A Conserved Archaeal Pathway for TailAnchored Membrane Protein…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/70_Sherrill_J_Traffic_2011.pdf
    11 Nov 2011: To test this, we first determinedthe X-ray crystal structure of ADP•AlF4-bound TRC40from the methanogen Methanothermobacter thermau-totrophicus at 2.1 Å resolution (Table S1, Figure S1 andMethods). ... To test this, we used a protease-protection
  37. Twinning and other pathologies Andrey Lebedev CCP4 OD-­‐structures…

    https://www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&refresh=6681665f5eb751719756383
    11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.
  38. doi:10.1016/j.cell.2008.11.004

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/55_Kang_SW_Cell_2008.pdf
    21 Nov 2008: Exami-nation of differences between related species offers a glimpse at the history of evolutionary processes, but monitor-ing evolution in a test tube allows deeper exploration of these processes as
  39. 15591370691369 1..30

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf
    29 May 2019: To test whether the role of TMED10 in GFP-PrP degradation depends on complex formation, we.
  40. Asgard archaea shed light on the evolutionary origins of the…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hatano%20Nat%20Comms%202022.pdf
    13 Jun 2022: To test for physical interactions between these putativeESCRT-I proteins, we recombinantly expressed in E. ... To test this idea, weused size-exclusion chromatography (Fig. 3A) and SEC-MALS(Fig.
  41. The EMBO Journal Vol.17 No.18 pp.5273–5285, 1998 Crystal structure ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1998.pdf
    9 Sep 1998: We sought to test whether this specialproperty could be explained by a change in the multimeriz-ation state of dynamin upon amphiphysin binding. ... To test the effect of the amphiphysins ondynamin multimerization, various recombinant SH3domains (5µg
  42. Molecular Biology of the CellVol. 8, 2003–2015, October 1997 ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/WiggeKohler_McMahon1997.pdf
    10 Oct 1997: To test this directly, Amph2 immunoprecipi-tates were Coomassie-stained, revealing that Amph1. ... This provided us with a useful tool to test theimportance of the heterodimer in vivo.
  43. Systematic and Statistical Analysis to Aid Improvement and Validation …

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/2014_Oct_LIGANDS.pdf
    10 Oct 2014: optimise functionality; streamline feedback cycle. • Test suitability of merging up the hierarchy (when appropriate). • ... bias. Concerns Raised: • Potential for cross-validation:. – Is retaining a “test set” of molecules appropriate? –
  44. Molecular Biology of the CellVol. 21, 3054 –3069, September ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/RVS161_MBC2010.pdf
    21 Feb 2011: To test these activities, wecoexpressed full-length Rvs161 and Rvs167 using an E. ... To-gether, our biochemical tests show that Rvs161-Rvs167 bindsto membranes and induces three-dimensional curvature.
  45. The patterned assembly and stepwise Vps4-mediated disassembly of…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf
    16 Jul 2023: S1E). As a further test of thisfinding, we expressed the Vps4E209Q plasmid in a cell line carryinga thymidine kinase homolog (hereafter called STK). ... 3A).To test the functional significance of this patterned assembly ofESCRT-III polymers, we used
  46. Mechanism of endophilin N-BAR domain-mediated membrane curvature…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/GallopEndophilin2006.pdf
    8 Jun 2006: implying the formation of additional helical structures. To test. how the predicted N-terminal amphipathic helix folds,. ... seeResults). Student’s t-test Po0.001, Po0.2. Endophilin and vesicle buddingJL Gallop et al. &
  47. JCB: Article The Rockefeller University Press $30.00J. Cell…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/63_Rane_NS_JCB_2010.pdf
    16 Feb 2010: Fortuitously, certain disease-causing prion protein (PrP) mutants meet these requirements and afford a unique opportunity to test whether in vivo signal sequences display either appreciable inef"ciency or functional differences. ... To test this idea
  48. No Job Name

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreC%20MM%202006.pdf
    7 Dec 2006: To test for dimerization invivo we turned to the bacterial two-hybrid system of Kari-mova et al. ... We thereforedecided to test whether B. subtilis MreC interacteddirectly with various PBPs.
  49. Biochem. J. (2011) 440, 185–193 (Printed in Great Britain) ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/ChernomordikBJ2011.pdf
    15 Nov 2011: kDa dextran were significantly different (paired Student’s t test, P < 0.001,n = 6). ... We used two experimental approaches, transfection andmicroinjection, to test the effects of this domain on HA-initiatedsyncytium formation.
  50. MinCD cell division proteins form alternating copolymeric cytomotive…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ncomms6341.pdf
    6 Dec 2014: 1a,d). MinC and MinD copolymerize to form an alternatingcopolymer. To experimentally test filament formation, we usedfull-length, untagged E. ... To test the effect of MinE on MinCD filaments,. we added MinE to the same experiment as described in Fig.
  51. Monitoring chaperone engagement of substratesin the endoplasmic…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/39_Snapp_EL_PNAS_2006.pdf
    21 Sep 2006: Statistical comparisons of Deff values were performedby using two-tailed Student t tests.Statistically significant change (P 0.005) relative to the steady-state. ... Fluorescencerecovery curves were plotted by using KALEIDAGRAPH 3.5 (SynergySoftware). We

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