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13 Jun 2009: 2.4. Northern blotting. RNA was extracted from cells using RNA STAT-60 (Tel-Test Inc.,Friendswood, TX). ... 2A). To test the functionality of the R383A and R383G mutants, flowcytometry was performed after incubating cells with the ABCG2-substrates

3 Sep 2008: some uncertainty of the molecular interpretation of the filaments,. so we carried out site-specific mutagenesis to test the contacts.

23 Jan 2012: Three-dimensional Structure of the Acetylcholine Receptor by Cryoelectron Microscopy and Helical Image Reconstruction C h i k a s h i T o y o s h i m a a n d N i g e l U n w i n. Medical Research Council Laboratory of Molecular Biology, Cambridge

9 Nov 2001: For complementation tests, amph26 homozygoteswere crossed to Sin3A/CyO mutant stocks, and non-curlyprogeny were assessed for amph26 mutant phenotypes.

15 May 2007: To directly test the. role of each residue of the IVF motif, in the context of. ... Methods. Errors bars were obtained by calculating the standard error and relevance of the difference assessed with a Student t-test.

23 May 2012: Wewere unable to test Trc35 in this assaybecause we could not purify Trc35 dueto poor expression and solubility. ... To test the involvementof the Bag6 complex in this process, we treated permeabilizedA9 cells with high salt, which removed most

30 Jul 2002: To test this hypothesis, HeLa cellswere fixed and stained for Eps15 (Figures 4A and 4B)and both AP1 and AP2 (Figures 4C and 4D) using anantibody against 1- and 2-adaptins.

1 May 2004: At least theparC/ParR/M system is simple enoughto test this possibility in vitro.

16 Jul 2008: To test the involvement of thispathway in PrP degradation by A4 cells, we analyzed PrPmetabolism upon inhibition of ERAD at two distinct stepswith either a proteasome inhibitor (MG132) or mannosidaseI inhibitor ... To test this idea, we generated a

19 Dec 2016: crenactin filaments. To test this, we performed. the same experiment using a peptide spanning.

7 Mar 2012: The reason for this is probably that the C-terminal amphipathic helix needs to be in contact with the membrane for polymerisation to occur at test-tube concentrations and in the ... Obviously, there are limitations as to what is possible, determined by

30 Apr 2010: 2. Layer this onto 100 µL of the sucrose cushion in an ultra-centrifuge tube (either a tube for the TLA120.1 rotor or amicro-test tube for the TL100.3 ... 1. Using glass measuring tools (e.g., glass pipettes), transfer20 mg PC and 5 mg PE to a glass

17 Mar 2003: In order to test the validity of this hypoth-by six residues to complete the b strand, resulted in a.

6 Feb 2003: To test whether the tetrameric TRAP complex alone wasthe component that stimulates. ... To test this, we analyzed theTRAP dependence of two point mutations in the PrP signalsequence (termed N7 and N9 [Fig.

21 Feb 2011: Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test.

12 Jan 2012: V3000 has not beentested yet. If somebody wants to test please let me know.

16 Oct 2003: To test this, we fused GFP or YFP to thecytoplasmic domains of three resident ER membrane proteinswith minimal cytoplasmic domains: two rough ER com-ponents of the translocon, Sec61. ... chimera. To test if OSER-inducing, GFP-tagged ER proteinswere

20 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly).

30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig.

30 May 2008: Experiments must now be designed to test this hypothesis. Translocational pausing has been viewed as a mecha- nism by which particular regions of the nascent chain can be isolated from the

21 Sep 2006: Meanwhile, the most definitive test for prions, a bioassay, was be-ing performed in parallel on comparable samples3639. ... These toolscan then be used in vivo to test specific hypotheses regarding the patho-genesis of prion diseases.

2 Feb 2004: 4) have been used asassembly tests for other members of the dynamin family(see Oligomerization web site in Online links). ... This clathrin-centricmodel for endocytosis does not, however, take intoaccount the role of dynamins in

29 Dec 2004: In order to test whether stimulated [3H]bumetanide binding would be re- tained in membranes after activation of transport by agents other than catecholamines, we examined specific binding in membranes from

22 Jul 2007: In addition,. using mouse models as a preclinical test, as we have done in.

24 Jan 2005: The model-derivedphases provided a more sensitive test of the signalretained in the images at high resolution than theprevious reference, which had been derived solelyfrom the images.

7 Jul 1999: The residuals betweenthe tted absorbance and the experimental values wereplotted, to test for the validity of the model used.

20 Nov 1997: Although the signifi-. cance of these findings remains to be determined, the Indeed, the contradicting conclusions resulting frommany of the studies which each used different test sub-rearrangements of the

3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared

20 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly).

27 Apr 2015: To test for seeding, we followed filament formation onimmobilized TubRC complexes (Fig.

31 Mar 2014: This essentially tests whether the. Tutorial 6: Linear Regression 4. model has any predictive power. ... b) Create a model to test this hypothesis, assuming a linear relationshipbetween Height and Girth.

10 Jan 2013: Test set size (%) 5.4. Rwork (%) 19.6(22.9). Rfree (%) 25.2(23.8). ... Significance was calculated using the Student’s t test( = p,0.05). D: Confocal micrographs of RAM cells over-expressing Bin2-EGFP (Bin2, green, left) or control (EGFP, green, right).

30 Jan 2004: T-tubule net-work (7 ). To test if this tubulating ability is dueto the Drosophila amphiphysin BAR domain,we mutated pairs of basic residues to gluta-mates, Lys161 Lys163 on the ... S3B). As a functional test of the amphiphysin BARdomain, we tested the

26 Jun 2001: The present work has generated a wide arrayof tools to test this and other hypotheses in future studies.

3 Dec 2005: previously used for the egg detection test and 20 new normal wild type videos. ... type. To test whether unc-29(x29) was clustering in the correct region, data obtained.

23 Oct 2012: parenthesis. † Rmerge = ΣΣ | Ihl - 〈Ih〉 | / ΣΣ 〈Ih〉 h l h l ‡ 5 % of the reflections were included in the test set.

28 Nov 2008: The error bars show 95% confidence intervals (calculated by t tests) for each condition.

4 Aug 2017: Fig. 2. Reconstitution ofUBE2O-mediated clientubiquitination with purifiedfactors. (A) Experimentalstrategy to test UBE2O inter-action and ubiquitination in adefined system.

23 Jul 2007: To further test this notion, we performed two independent. measurements. First, we monitored by SPR analysis the.

17 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. •

7 Apr 2010: FEBS Letters 584 (2010) 1830–1839. j o u r n a l h o m e p a g e : w w w. F E B S L e t t e r s. o r g. Review. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains. Felix Campelo a,b, Gur Fabrikant a, Harvey T. McMahon c,

20 Apr 2002: of theδ1 enzyme show similar structural features, it was of interestto test the mammalianδ1 enzyme for activation by organicsolvent.

24 Jan 2009: significantly (p 0.002)from wild-type and Syt-1RK by a general linear model analysis of variance using the Tukey post hoc test of values.

30 Jan 2004: T-tubule net-work (7 ). To test if this tubulating ability is dueto the Drosophila amphiphysin BAR domain,we mutated pairs of basic residues to gluta-mates, Lys161 Lys163 on the ... S3B). As a functional test of the amphiphysin BARdomain, we tested the

24 Sep 2015: Statistical analysisThe Student t test was carried out for each experiment.

8 Nov 2004: To test this possibility, we analyzed the behavior of PrP. constructs containing mutations in the transmembrane do-.

24 Sep 2015: previous report (15). We wanted to test whether a model withtwo ER compartments that are functionally distinct but slowlyequilibrate with each through diffusion of Ca2!

1 Dec 2010: generate sister chromatid cohesion. To test the effect of charge neutralization on cohesin complex.

27 Mar 2012: be the most reliable test for FtsA polymerization, as it shows.

20 Apr 2002: Data statistics are listed in Table1. Test data for the calculation of freeR factors of all thesedata sets were chosen to be consistent with the selection oftest data for the native